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. 2004 May;6(3):195–206. doi: 10.1593/neo.3370

Figure 3.

Figure 3

GSH depletion achieved by BSO pretreatment commits control cells to alkylating agent-induced apoptosis, without affecting the response to ADR and CPT. (A) Intracellular GSH content evaluated in the untreated M14 (●) and MN2 control (■) cells and BSO-treated M14 (○) and MN2 (□) cells before (-6 hours) and from 0 to 72 hours following exposure to BSO (10 mM; 6 hours). Statistical analysis: P < .01 at 24 to 72 hours after drug exposure for BSO-exposed control cells when compared to unexposed ones. (B) Cytofluorimetric evaluation of apoptotic (annexin V+/PI-) M14 and MN2 control cells preincubated (white bars) or not (gray bars) with BSO and treated with the IC50 doses of CDDP, l-PAM, ADR, or CPT. Analysis was performed from 24 to 72 hours following the end of the treatments. Control cells, both unexposed and exposed to BSO, did not show any apoptosis; thus, their values have not been included in the histograms. Statistical analysis: P < .05 at 24 hours, < .01 at 48 to 72 hours after exposure to CDDP and l-PAM calculated for BSO-exposed control cells when compared to unexposed ones.