Table 3.
Expression and Relative Levels of the hTERT and Alternatively Spliced Isoforms.
| Cell lines | hTERT mRNA* | Telomerase† activity | |||||||||
| No deletion | α | β | γ | αβ | αγ | βγ | αβγ | No deletion + β | Total hTERT | ||
| Huh 6 | 2.77 | 2.23 | 2.95 | 0.00 | 1.98 | 0.00 | 1.77 | 0.00 | 3.17 | 3.26 | 44 |
| (32.5%) | (9.4%) | (49.5%) | (0.0%) | (5.3%) | (0.0%) | (3.3%) | (0.0%) | (82.0%) | (100%) | ||
| Huh 7 | 3.28 | 1.93 | 4.03 | 1.79 | 3.08 | 1.57 | 2.06 | 0.00 | 4.10 | 4.15 | 76 |
| (13.4%) | (0.6%) | (75.9%) | (0.4%) | (8.6%) | (0.3%) | (0.8%) | (0.0%) | (89.3%) | (100%) | ||
| HLE | 3.29 | 2.30 | 3.84 | 2.32 | 2.89 | 0.00 | 2.04 | 1.93 | 3.95 | 4.01 | 69 |
| (18.9%) | (1.9%) | (67.7%) | (2.0%) | (7.5%) | (0.0%) | (1.1%) | (0.8%) | (86.6%) | (100%) | ||
| LI 90 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0 |
| (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | ||
| HUV-EC-C | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0 |
| (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | ||
Upper row: Each mRNA was calculate as log copies per microgram of total RNA; lower row: relative level of each mRNA was calculated.
*
The means of triplicate determinations are shown.
†
The means of duplicate determinations are shown (TPG units).