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OA induces time- and concentration-dependent dephosphorylation of FAK. SH-EP cells were serum-starved for 4 hours and treated with increasing concentrations of OA for 4 hours (left panel), or with 250 nM OA for the indicated times (right panel). Equal amounts of protein from whole cell lysates were separated by SDS-PAGE, transferred to nitrocellulose, and analyzed by immunoblotting with the indicated antibodies (A–H).
