Figure 5.
Effect of pure antiestrogen ICI 182,780 on 2-ME2-induced upregulation of VEGF-A mRNA expression in GH3 cells. (A) GH3 cells were exposed to 1 µM 2-ME2 alone or in combination with 1 µM ICI 182,780 for 3 days. Total RNA was isolated and analyzed by Northern blotting using nonradioactive DIG-labeled PCRgenerated probes for VEGF-A and GAPDH. (B) The arbitrary values indicate VEGF-A mRNA concentrations. Data displayed as mean ± SD from three separate experiments. P value was determined by Student's t-test. *P < .01 vs control.