Figure 1.

Endogenous levels of FADD, BID, and procaspase 3 are higher in SW480 cells. Procaspase 8 and FLIP processing at the TRAIL-DISC. (A) Western analysis of the endogenous levels of the indicated proteins in NHH-2, NKNT-3, SW480 and HCT116 cells. (B) The cells were treated with His-TRAIL for 15 minutes (upper panel) or 30 minutes (lower panel) in the presence of the cross-linking antibody. The TRAIL-DISC complex was immunoprecipitated and tested for the presence of procaspase 8, FADD, and FLIP. “M” stands for mock, and “TR” or “T” for TRAIL treatment, as described in Materials and Methods.