Abstract
The rubella hemagglutination inhibition (hi) test is the most sensitive serologic method available for diagnosis of infection and for determination of immunity status. At present, however, it is not a well-standardized procedure and several modifications are in use. Comparison of results obtained on the same specimens by different laboratories has shown wide variations. The sensitivity and reliability of the technique is decidedly influenced by each of the variables of the test system; these include erythrocytes, antigen, methods employed for removal of non-specific inhibitors and natural agglutinins from test sera, the pH of the diluents and the temperature of incubation. The presence or absence of hi antibody cannot be determined reliably by screening a single low dilution of serum. Commercially available reagents and kits for rubella hi tests vary widely in their reliability. The test should be performed only by experienced persons who understand the principles of the technique and who are aware of the pitfalls and of the importance of using adequate controls in each run.
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Selected References
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- Cooper L. Z., Matters B., Rosenblum J. K., Krugman S. Experience with a modified rubella hemagglutination inhibition antibody test. JAMA. 1969 Jan 6;207(1):89–93. [PubMed] [Google Scholar]
- Feldman H. A. Removal by heparin-MnCl2 of nonspecific rubella hemagglutinin serum inhibitor. Proc Soc Exp Biol Med. 1968 Feb;127(2):570–573. doi: 10.3181/00379727-127-32743. [DOI] [PubMed] [Google Scholar]
- Halonen P. E., Ryan J. M., Stewart J. A. Rubella hemagglutinin prepared with alkaline extraction of virus grown in suspension culture of BHK-21 cells. Proc Soc Exp Biol Med. 1967 May;125(1):162–167. doi: 10.3181/00379727-125-32038. [DOI] [PubMed] [Google Scholar]
- Lennette E. H., Schmidt N. J., Magoffin R. L. The hemagglutination inhibition test for rubella: a comparison of its sensitivity to that of neutralization, complement fixation and fluorescent antibody tests for diagnosis of infection and determination of immunity status. J Immunol. 1967 Oct;99(4):785–793. [PubMed] [Google Scholar]
- Plotkin S. A., Bechtel D. J., Sedwick W. D. A simple method for removal of rubella hemagglutination inhibitors from serum adaptable to finger-tip blood. Am J Epidemiol. 1968 Nov;88(3):301–304. doi: 10.1093/oxfordjournals.aje.a120888. [DOI] [PubMed] [Google Scholar]
- Stewart G. L., Parkman P. D., Hopps H. E., Douglas R. D., Hamilton J. P., Meyer H. M., Jr Rubella-virus hemagglutination-inhibition test. N Engl J Med. 1967 Mar 9;276(10):554–557. doi: 10.1056/NEJM196703092761006. [DOI] [PubMed] [Google Scholar]