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. 1981 Jan 31;282(6261):351–354. doi: 10.1136/bmj.282.6261.351

Clinical usefulness of urinary 3-methylhistidine excretion in indicating muscle protein breakdown.

M Elia, A Carter, S Bacon, C G Winearls, R Smith
PMCID: PMC1504145  PMID: 6780020

Abstract

Urinary excretion of the post-translationally modified amino-acid 3-methylhistidine, derived from the contractile proteins actin and myosin, was measured in patients with conditions associated with nitrogen loss. The ratio of 3-methylhistidine:creatinine excretion, a measure of the fractional catabolic rate of myofibrillar protein was increased in severe injury, thyrotoxicosis, neoplastic disease, prednisolone administration, and sometimes Duchenne muscular dystrophy. In myxoedema, osteomalacia, and hypothermia the ratio was decreased; and starvation, elective operations, and rheumatoid arthritis had little effect. Provided that the diet is meat free, measurement of urinary 3-methylhistidine may provide useful information on the cause of protein loss.

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Selected References

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  1. Baker S. P., O'Neill B., Haddon W., Jr, Long W. B. The injury severity score: a method for describing patients with multiple injuries and evaluating emergency care. J Trauma. 1974 Mar;14(3):187–196. [PubMed] [Google Scholar]
  2. Ballard F. J., Tomas F. M., Stern L. M. Increased turnover of muscle contractile proteins in Duchenne muscular dystrophy as assessed by 3-methylhistidine and creatinine excretion. Clin Sci (Lond) 1979 Apr;56(4):347–352. doi: 10.1042/cs0560347. [DOI] [PubMed] [Google Scholar]
  3. Burman K. D., Wartofsky L., Dinterman R. E., Kesler P., Wannemacher R. W., Jr The effect of T3 and reverse T3 administration on muscle protein catabolism during fasting as measured by 3-methylhistidine excretion. Metabolism. 1979 Aug;28(8):805–813. doi: 10.1016/0026-0495(79)90206-3. [DOI] [PubMed] [Google Scholar]
  4. Crane C. W., Picou D., Smith R., Waterlow J. C. Protein turnover in patients before and after elective orthopaedic operations. Br J Surg. 1977 Feb;64(2):129–133. doi: 10.1002/bjs.1800640212. [DOI] [PubMed] [Google Scholar]
  5. Edwards R. H. Studies of muscular performance in normal and dystrophic subjects. Br Med Bull. 1980 May;36(2):159–164. doi: 10.1093/oxfordjournals.bmb.a071632. [DOI] [PubMed] [Google Scholar]
  6. Elia M., Carter A., Bacon S., Smith R. The effect of 3-methylhistidine in food on its urinary excretion in man. Clin Sci (Lond) 1980 Dec;59(6):509–511. doi: 10.1042/cs0590509. [DOI] [PubMed] [Google Scholar]
  7. Elia M., Carter A., Smith R. The 3-methylhistidine content of human tissues. Br J Nutr. 1979 Nov;42(3):567–570. doi: 10.1079/bjn19790149. [DOI] [PubMed] [Google Scholar]
  8. Funabiki R., Watanabe Y., Nishizawa N., Hareyama S. Quantitative aspect of the myofibrillar protein turnover in transient state on dietary protein depletion and repletion revealed by urinary excretion of N7-methylhistid;ne. Biochim Biophys Acta. 1976 Nov 18;451(1):143–150. doi: 10.1016/0304-4165(76)90266-x. [DOI] [PubMed] [Google Scholar]
  9. Goldberg A. L. Protein turnover in skeletal muscle. II. Effects of denervation and cortisone on protein catabolism in skeletal muscle. J Biol Chem. 1969 Jun 25;244(12):3223–3229. [PubMed] [Google Scholar]
  10. Haverberg L. N., Deckelbaum L., Bilmazes C., Munro H. N., Young V. R. Myofibrillar protein turnover and urinary N-tau-methylhistidine output. Response to dietary supply of protein and energy. Biochem J. 1975 Dec;152(3):503–510. doi: 10.1042/bj1520503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Inoue R., Miyake M., Kanazawa A., Sato M., Kakimoto Y. Decrease of 3-methylhistidine and increase of NG,NG-dimethylarginine in the urine of patients with muscular dystrophy. Metabolism. 1979 Aug;28(8):801–804. doi: 10.1016/0026-0495(79)90205-1. [DOI] [PubMed] [Google Scholar]
  12. Jefferson L. S., Rannels D. E., Munger B. L., Morgan H. E. Insulin in the regulation of protein turnover in heart and skeletal muscle. Fed Proc. 1974 Apr;33(4):1098–1104. [PubMed] [Google Scholar]
  13. Long C. L., Haverberg L. N., Young V. R., Kinney J. M., Munro H. N., Geiger J. W. Metabolism of 3-methylhistidine in man. Metabolism. 1975 Aug;24(8):929–935. doi: 10.1016/0026-0495(75)90084-0. [DOI] [PubMed] [Google Scholar]
  14. Long C. L., Schiller W. R., Blakemore W. S., Geiger J. W., O'Dell M., Henderson K. Muscle protein catabolism in the septic patient as measured by 3-methylhistidine excretion. Am J Clin Nutr. 1977 Aug;30(8):1349–1352. doi: 10.1093/ajcn/30.8.1349. [DOI] [PubMed] [Google Scholar]
  15. Marliss E. B., Murray F. T., Nakhooda A. F. The metabolic response to hypocaloric protein diets in obese man. J Clin Invest. 1978 Aug;62(2):468–479. doi: 10.1172/JCI109148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McKeran R. O., Halliday D., Purkiss P. Increased myofibrillar protein catabolism in Duchenne muscular dystrophy measured by 3-methylhistidine excretion in the urine. J Neurol Neurosurg Psychiatry. 1977 Oct;40(10):979–981. doi: 10.1136/jnnp.40.10.979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McKeran R. O., Halliday D., Purkiss P., Royston P. 3-Methylhistidine excretion as an index of myofibrillar protein catabolism in neuromuscular disease. J Neurol Neurosurg Psychiatry. 1979 Jun;42(6):536–541. doi: 10.1136/jnnp.42.6.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Millward D. J., Bates P. C., Grimble G. K., Brown J. G., Nathan M., Rennie M. J. Quantitative importance of non-skeletal-muscle sources of N tau-methylhistidine in urine. Biochem J. 1980 Jul 15;190(1):225–228. doi: 10.1042/bj1900225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Munro H. N. Nutrition and muscle protein metabolism: Introduction. Fed Proc. 1978 Jul;37(9):2281–2282. [PubMed] [Google Scholar]
  20. Nishizawa N., Noguchi T., Hareyama S., Funabiki R. Fractional flux rates of Nt-methylhistidine in skin and gastrointestine: the contribution of these tissues to urinary excretion of Nt-methylhistidine in the rat. Br J Nutr. 1977 Jul;38(1):149–151. doi: 10.1079/bjn19770072. [DOI] [PubMed] [Google Scholar]
  21. Rao B. S., Nagabhushan V. S. Urinary excretion of 3-methyl histidine in children suffering from protein-calorie malnutrition. Life Sci II. 1973 Mar 8;12(5):205–210. doi: 10.1016/0024-3205(73)90348-2. [DOI] [PubMed] [Google Scholar]
  22. Tomas F. M., Ballard F. J., Pope L. M. Age-dependent changes in the rate of myofibrillar protein degradation in humans as assessed by 3-methylhistidine and creatinine excretion. Clin Sci (Lond) 1979 Apr;56(4):341–346. doi: 10.1042/cs0560341. [DOI] [PubMed] [Google Scholar]
  23. Wannemacher R. W., Jr, Dinterman R. E., Pekarek R. S., Bartelloni P. J., Beisel W. R. Urinary amino acid excretion during experimentally induced sandfly fever in man. Am J Clin Nutr. 1975 Feb;28(2):110–118. doi: 10.1093/ajcn/28.2.110. [DOI] [PubMed] [Google Scholar]
  24. Whitehouse S., Katz N., Schaeffer G., Kluthe R. Histidines and renal function. Clin Nephrol. 1975 Jan;3(1):24–27. [PubMed] [Google Scholar]
  25. Williamson D. H., Farrell R., Kerr A., Smith R. Muscle-protein catabolism after injury in man, as measured by urinary excretion of 3-methylhistidine. Clin Sci Mol Med. 1977 May;52(5):527–533. doi: 10.1042/cs0520527. [DOI] [PubMed] [Google Scholar]
  26. Young V. R., Havenberg L. N., Bilmazes C., Munro H. N. Potential use of 3-methylhistidine excretion as an index of progressive reduction in muscle protein catabolism during starvation. Metabolism. 1973 Nov;23(2):1429–1436. doi: 10.1016/0026-0495(73)90257-6. [DOI] [PubMed] [Google Scholar]
  27. Young V. R., Munro H. N. Ntau-methylhistidine (3-methylhistidine) and muscle protein turnover: an overview. Fed Proc. 1978 Jul;37(9):2291–2300. [PubMed] [Google Scholar]

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