Figure 6.

Analysis of the site of integration of the pNCT-tel plasmid in clone B3 using fluorescence in situ hybridization. (A) A single integration site of the pNCT-tel plasmid is observed in clone B3 at the end of the short arm of a chromosome. Hybridization was performed with the pNCT-Δ plasmid and chromosomes were counterstained with propidium iodide. The chromosome was identified by chromosome-specific probes to be chromosome 16 (data not shown). (B) Hybridization with both a telomere-specific PNA probe (red) and a chromosome-16-specific probe (green) demonstrated that telomeric repeat sequences are located on the ends of both homologs of chromosome 16 in clone B3. Chromosomes were counterstained with DAPI. (C) The hybridization signal observed with the telomere-specific probe alone showing detectable telomeres on both ends of all the chromosomes.