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. Author manuscript; available in PMC: 2006 Jul 17.
Published in final edited form as: Oncogene. 2005 Sep 29;24(43):6482–6491. doi: 10.1038/sj.onc.1208810

Figure 2.

Figure 2

RT–PCR detection of mRNA for NF-κB-mediated genes following detachment. Cells were detached and established on poly-HEMA-coated plates for the length of time indicated on the gel and then harvested and total RNA isolated. The primer sequences used for detection of each molecule predicted to increase by microarray are available in Supplementary Table 2. The experiment was conducted twice using cells on poly-HEMA and once using cells in roller culture, and all the molecules increased in all the experiments