Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1994 Sep;113(1):35–42. doi: 10.1111/j.1476-5381.1994.tb16170.x

Investigation of the endogenous chemoattractants involved in 111In-eosinophil accumulation in passive cutaneous anaphylactic reactions in the guinea-pig.

V B Weg 1, M L Watson 1, L H Faccioli 1, T J Williams 1
PMCID: PMC1510071  PMID: 7812629

Abstract

1. Eosinophil accumulation and plasma extravasation are features of type I allergic responses. In an attempt to characterize the mediators of these responses, we have examined the local accumulation of 111In-eosinophils and leakage of 125I-human serum albumin (125I-HSA) during passive cutaneous anaphylaxis (PCA) reactions and in response to defined inflammatory mediators in the guinea-pig. Animals were passively sensitized by intradermal injection of anti-bovine gamma globulin antibody (50 microliters, 1/50 dilution). After 20-24 h, animals were injected intravenously with 111In-eosinophils and 125I-HSA for the measurement of cell accumulation and plasma leakage, respectively. 2. When injected into sensitized sites, antigen caused a dose-related increase in the accumulation of 111In-eosinophils and plasma leakage in guinea-pig skin. Time course experiments over 24 h revealed that the maximal rate of 111In-eosinophil accumulation occurred over the first 90 min, with little accumulation at later time points. Plasma leakage was completed within the first 30 min after challenge. Responses to the mast cell degranulator, compound 48/80, exhibited very similar responses to the PCA reaction. 3. Co-injection of antigen with the PAF antagonist, WEB 2086 (10(-7) mol/site) or the 5-lipoxygenase inhibitor, PF 5901 (10(-7) mol/site) did not significantly alter the accumulation of 111In-eosinophils or plasma leakage, whereas these drug doses abolished responses to exogenous PAF (10(-9) mol/site) and arachidonic acid (AA, 3 x 10(-8) mol/site), respectively. The H1 receptor antagonist chlorpheniramine (2.5 x 10(-8) mol/site) did not reduce antigen-induced 111In-eosinophil accumulation.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
35

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkins P., Green G. R., Zweiman B. Histologic studies of human skin test responses to ragweed, compound 48-80, and histamine. J Allergy Clin Immunol. 1973 May;51(5):263–273. doi: 10.1016/0091-6749(73)90128-0. [DOI] [PubMed] [Google Scholar]
  2. Braquet P., Touqui L., Shen T. Y., Vargaftig B. B. Perspectives in platelet-activating factor research. Pharmacol Rev. 1987 Jun;39(2):97–145. [PubMed] [Google Scholar]
  3. Chan C. C., McKee K., Tagari P., Chee P., Ford-Hutchinson A. Eosinophil-eicosanoid interactions: inhibition of eosinophil chemotaxis in vivo by a LTD4-receptor antagonist. Eur J Pharmacol. 1990 Dec 4;191(3):273–280. doi: 10.1016/0014-2999(90)94159-u. [DOI] [PubMed] [Google Scholar]
  4. Church M. K., el-Lati S., Okayama Y. Biological properties of human skin mast cells. Clin Exp Allergy. 1991 May;21 (Suppl 3):1–9. doi: 10.1111/j.1365-2222.1991.tb01757.x. [DOI] [PubMed] [Google Scholar]
  5. Clark R. A., Gallin J. I., Kaplan A. P. The selective eosinophil chemotactic activity of histamine. J Exp Med. 1975 Dec 1;142(6):1462–1476. doi: 10.1084/jem.142.6.1462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Collins P. D., Weg V. B., Faccioli L. H., Watson M. L., Moqbel R., Williams T. J. Eosinophil accumulation induced by human interleukin-8 in the guinea-pig in vivo. Immunology. 1993 Jun;79(2):312–318. [PMC free article] [PubMed] [Google Scholar]
  7. Czarnetzki B. M., Mertensmeier R. In vitro and in vivo chemotaxis of guinea pig leukocytes toward leukotriene B4 and its w-oxidation products. Prostaglandins. 1985 Jul;30(1):5–11. doi: 10.1016/s0090-6980(85)80006-x. [DOI] [PubMed] [Google Scholar]
  8. De Monchy J. G., Kauffman H. F., Venge P., Koëter G. H., Jansen H. M., Sluiter H. J., De Vries K. Bronchoalveolar eosinophilia during allergen-induced late asthmatic reactions. Am Rev Respir Dis. 1985 Mar;131(3):373–376. doi: 10.1164/arrd.1985.131.3.373. [DOI] [PubMed] [Google Scholar]
  9. Diaz P., Gonzalez M. C., Galleguillos F. R., Ancic P., Cromwell O., Shepherd D., Durham S. R., Gleich G. J., Kay A. B. Leukocytes and mediators in bronchoalveolar lavage during allergen-induced late-phase asthmatic reactions. Am Rev Respir Dis. 1989 Jun;139(6):1383–1389. doi: 10.1164/ajrccm/139.6.1383. [DOI] [PubMed] [Google Scholar]
  10. Dor P. J., Vervloet D., Sapene M., Andrac L., Bonerandi J. J., Charpin J. Induction of late cutaneous reaction by kallikrein injection: comparison with allergic-like late response to compound 48/80. J Allergy Clin Immunol. 1983 Apr;71(4):363–370. doi: 10.1016/0091-6749(83)90063-5. [DOI] [PubMed] [Google Scholar]
  11. Ennis M. Histamine release from human pulmonary mast cells. Agents Actions. 1982 Apr;12(1-2):60–63. doi: 10.1007/BF01965108. [DOI] [PubMed] [Google Scholar]
  12. Ennis M., Pearce F. L. Differential reactivity of isolated mast cells from the rat and guinea pig. Eur J Pharmacol. 1980 Sep 5;66(4):339–345. doi: 10.1016/0014-2999(80)90466-5. [DOI] [PubMed] [Google Scholar]
  13. Faccioli L. H., Nourshargh S., Moqbel R., Williams F. M., Sehmi R., Kay A. B., Williams T. J. The accumulation of 111In-eosinophils induced by inflammatory mediators, in vivo. Immunology. 1991 Jun;73(2):222–227. [PMC free article] [PubMed] [Google Scholar]
  14. Ford-Hutchinson A. W., Bray M. A., Doig M. V., Shipley M. E., Smith M. J. Leukotriene B, a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytes. Nature. 1980 Jul 17;286(5770):264–265. doi: 10.1038/286264a0. [DOI] [PubMed] [Google Scholar]
  15. Gordon J. R., Burd P. R., Galli S. J. Mast cells as a source of multifunctional cytokines. Immunol Today. 1990 Dec;11(12):458–464. doi: 10.1016/0167-5699(90)90176-a. [DOI] [PubMed] [Google Scholar]
  16. Gulbenkian A. R., Fernandez X., Kreutner W., Minnicozzi M., Watnick A. S., Kung T., Egan R. W. Anaphylactic challenge causes eosinophil accumulation in bronchoalveolar lavage fluid of guinea pigs. Modulation by betamethasone, phenidone, indomethacin, WEB 2086, and a novel antiallergy agent, SCH 37224. Am Rev Respir Dis. 1990 Sep;142(3):680–685. doi: 10.1164/ajrccm/142.3.680. [DOI] [PubMed] [Google Scholar]
  17. Henocq E., Vargaftig B. B. Skin eosinophilia in atopic patients. J Allergy Clin Immunol. 1988 Apr;81(4):691–695. doi: 10.1016/0091-6749(88)91040-8. [DOI] [PubMed] [Google Scholar]
  18. Hirashima M., Tashiro K., Hirotsu Y., Hayashi H. The mediation of tissue eosinophilia in hypersensitivity reactions. V. Comparative study of tissue eosinophilia in the skin lesions of local and systemic passive cutaneous anaphylactic reactions. Immunology. 1983 Sep;50(1):85–91. [PMC free article] [PubMed] [Google Scholar]
  19. Kameyoshi Y., Dörschner A., Mallet A. I., Christophers E., Schröder J. M. Cytokine RANTES released by thrombin-stimulated platelets is a potent attractant for human eosinophils. J Exp Med. 1992 Aug 1;176(2):587–592. doi: 10.1084/jem.176.2.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kay A. B., Corrigan C. J. Asthma. Eosinophils and neutrophils. Br Med Bull. 1992 Jan;48(1):51–64. doi: 10.1093/oxfordjournals.bmb.a072541. [DOI] [PubMed] [Google Scholar]
  21. Kay A. B. Studies on eosinophil leucocyte migration. I. Eosinophil and neutrophil accumulation following antigen-antibody reactions in guinea-pig skin. Clin Exp Immunol. 1970 Jan;6(1):75–86. [PMC free article] [PubMed] [Google Scholar]
  22. Leiferman K. M., Fujisawa T., Gray B. H., Gleich G. J. Extracellular deposition of eosinophil and neutrophil granule proteins in the IgE-mediated cutaneous late phase reaction. Lab Invest. 1990 May;62(5):579–589. [PubMed] [Google Scholar]
  23. Lellouch-Tubiana A., Lefort J., Simon M. T., Pfister A., Vargaftig B. B. Eosinophil recruitment into guinea pig lungs after PAF-acether and allergen administration. Modulation by prostacyclin, platelet depletion, and selective antagonists. Am Rev Respir Dis. 1988 Apr;137(4):948–954. doi: 10.1164/ajrccm/137.4.948. [DOI] [PubMed] [Google Scholar]
  24. Lozewicz S., Gomez E., Chalstrey S., Gatland D., Davies R. J. Time course of cellular infiltration in the nasal mucosa during the immediate allergic reaction. Int Arch Allergy Appl Immunol. 1991;95(2-3):273–277. doi: 10.1159/000235441. [DOI] [PubMed] [Google Scholar]
  25. Nourshargh S. Mechanisms of neutrophil and eosinophil accumulation in vivo. Am Rev Respir Dis. 1993 Dec;148(6 Pt 2):S60–S64. doi: 10.1164/ajrccm/148.6_Pt_2.S60. [DOI] [PubMed] [Google Scholar]
  26. Nourshargh S., Rampart M., Hellewell P. G., Jose P. J., Harlan J. M., Edwards A. J., Williams T. J. Accumulation of 111In-neutrophils in rabbit skin in allergic and non-allergic inflammatory reactions in vivo. Inhibition by neutrophil pretreatment in vitro with a monoclonal antibody recognizing the CD18 antigen. J Immunol. 1989 May 1;142(9):3193–3198. [PubMed] [Google Scholar]
  27. Ogawa H., Kunkel S. L., Fantone J. C., Ward P. A. Comparative study of eosinophil and neutrophil chemotaxis and enzyme release. Am J Pathol. 1981 Nov;105(2):149–155. [PMC free article] [PubMed] [Google Scholar]
  28. Ovary Z., Kaplan B., Kojima S. Characteristics of guinea pig IgE. Int Arch Allergy Appl Immunol. 1976;51(4):416–428. doi: 10.1159/000231616. [DOI] [PubMed] [Google Scholar]
  29. Perini A., Mota I. Heterogeneity of guinea-pig homocytotropic antibodies. Immunology. 1972 May;22(5):915–923. [PMC free article] [PubMed] [Google Scholar]
  30. Plaut M., Pierce J. H., Watson C. J., Hanley-Hyde J., Nordan R. P., Paul W. E. Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores. Nature. 1989 May 4;339(6219):64–67. doi: 10.1038/339064a0. [DOI] [PubMed] [Google Scholar]
  31. Richards I. M., Griffin R. L., Oostveen J. A., Morris J., Wishka D. G., Dunn C. J. Effect of the selective leukotriene B4 antagonist U-75302 on antigen-induced bronchopulmonary eosinophilia in sensitized guinea pigs. Am Rev Respir Dis. 1989 Dec;140(6):1712–1716. doi: 10.1164/ajrccm/140.6.1712. [DOI] [PubMed] [Google Scholar]
  32. Rot A., Krieger M., Brunner T., Bischoff S. C., Schall T. J., Dahinden C. A. RANTES and macrophage inflammatory protein 1 alpha induce the migration and activation of normal human eosinophil granulocytes. J Exp Med. 1992 Dec 1;176(6):1489–1495. doi: 10.1084/jem.176.6.1489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sehmi R., Cromwell O., Taylor G. W., Kay A. B. Identification of guinea pig eosinophil chemotactic factor of anaphylaxis as leukotriene B4 and 8(S),15(S)-dihydroxy-5,9,11,13(Z,E,Z,E)-eicosatetraenoic acid. J Immunol. 1991 Oct 1;147(7):2276–2283. [PubMed] [Google Scholar]
  34. Silbaugh S. A., Stengel P. W., Williams G. D., Herron D. K., Gallagher P., Baker S. R. Effects of leukotriene B4 inhalation. Airway sensitization and lung granulocyte infiltration in the guinea pig. Am Rev Respir Dis. 1987 Oct;136(4):930–934. doi: 10.1164/ajrccm/136.4.930. [DOI] [PubMed] [Google Scholar]
  35. Wardlaw A. J., Moqbel R., Cromwell O., Kay A. B. Platelet-activating factor. A potent chemotactic and chemokinetic factor for human eosinophils. J Clin Invest. 1986 Dec;78(6):1701–1706. doi: 10.1172/JCI112765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Watson M. L., Smith D., Bourne A. D., Thompson R. C., Westwick J. Cytokines contribute to airway dysfunction in antigen-challenged guinea pigs: inhibition of airway hyperreactivity, pulmonary eosinophil accumulation, and tumor necrosis factor generation by pretreatment with an interleukin-1 receptor antagonist. Am J Respir Cell Mol Biol. 1993 Apr;8(4):365–369. doi: 10.1165/ajrcmb/8.4.365. [DOI] [PubMed] [Google Scholar]
  37. Weg V. B., Watson M. L., Cordeiro R. S., Williams T. J. Histamine, leukotriene D4 and platelet-activating factor in guinea pig passive cutaneous anaphylaxis. Eur J Pharmacol. 1991 Nov 5;204(2):157–163. doi: 10.1016/0014-2999(91)90700-z. [DOI] [PubMed] [Google Scholar]
  38. Weg V. B., Williams T. J., Lobb R. R., Nourshargh S. A monoclonal antibody recognizing very late activation antigen-4 inhibits eosinophil accumulation in vivo. J Exp Med. 1993 Feb 1;177(2):561–566. doi: 10.1084/jem.177.2.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Wegner C. D., Gundel R. H., Reilly P., Haynes N., Letts L. G., Rothlein R. Intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of asthma. Science. 1990 Jan 26;247(4941):456–459. doi: 10.1126/science.1967851. [DOI] [PubMed] [Google Scholar]
  40. Wershil B. K., Wang Z. S., Gordon J. R., Galli S. J. Recruitment of neutrophils during IgE-dependent cutaneous late phase reactions in the mouse is mast cell-dependent. Partial inhibition of the reaction with antiserum against tumor necrosis factor-alpha. J Clin Invest. 1991 Feb;87(2):446–453. doi: 10.1172/JCI115016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Wodnar-Filipowicz A., Heusser C. H., Moroni C. Production of the haemopoietic growth factors GM-CSF and interleukin-3 by mast cells in response to IgE receptor-mediated activation. Nature. 1989 May 11;339(6220):150–152. doi: 10.1038/339150a0. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES