Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1995 Feb;114(4):888–894. doi: 10.1111/j.1476-5381.1995.tb13287.x

Acute effects of the beta 3-adrenoceptor agonist, BRL 35135, on tissue glucose utilisation.

Y L Liu 1, M J Stock 1
PMCID: PMC1510208  PMID: 7773551

Abstract

1. The acute effects of BRL 35135 (BRL) on tissue glucose utilisation index (GUI) in vivo were investigated in anaesthetized rats by use of 2-deoxy-[3H]-glucose. 2. Intravenous injection of BRL caused a dose-dependent increase in GUI in skeletal muscle, and white and brown adipose tissue; plasma insulin and fatty acid concentrations were also increased. Chronic treatment with BRL added to the diet caused a 34 fold increase in basal GUI of brown adipose tissue (BAT), but had no effect on GUI in other tissues. After chronic treatment, the acute tissue response to an intravenous maximal dose of BRL had disappeared completely in all tissues apart from the soleus muscle. 3. A high dose (20 mg kg-1) of the non-selective beta-antagonist, propranolol, inhibited the acute effect of BRL on GUI in BAT, but failed to affect GUI in muscle. A lower dose (1 mg kg-1) of the antagonist also inhibited the BAT response, but had little or no effect on the response in Type I (working) muscles such as soleus and adductor longus (ADL), and potentiated the response in Type II (non-working) muscles such as tibialis and extensor digitorium longus (EDL). 4. A low dose (1 mg kg-1) of the selective beta 1-antagonist, atenolol, had no effect on the BRL response but the same dose of the selective beta 2-antagonist, ICI 118551, potentiated significantly the effect of BRL on GUI in most muscles without altering plasma insulin levels.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
888

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe H., Minokoshi Y., Shimazu T. Effect of a beta 3-adrenergic agonist, BRL35135A, on glucose uptake in rat skeletal muscle in vivo and in vitro. J Endocrinol. 1993 Dec;139(3):479–486. doi: 10.1677/joe.0.1390479. [DOI] [PubMed] [Google Scholar]
  2. Arch J. R., Ainsworth A. T., Cawthorne M. A., Piercy V., Sennitt M. V., Thody V. E., Wilson C., Wilson S. Atypical beta-adrenoceptor on brown adipocytes as target for anti-obesity drugs. Nature. 1984 May 10;309(5964):163–165. doi: 10.1038/309163a0. [DOI] [PubMed] [Google Scholar]
  3. Arch J. R., Kaumann A. J. Beta 3 and atypical beta-adrenoceptors. Med Res Rev. 1993 Nov;13(6):663–729. doi: 10.1002/med.2610130604. [DOI] [PubMed] [Google Scholar]
  4. Baron A. D., Steinberg H., Brechtel G., Johnson A. Skeletal muscle blood flow independently modulates insulin-mediated glucose uptake. Am J Physiol. 1994 Feb;266(2 Pt 1):E248–E253. doi: 10.1152/ajpendo.1994.266.2.E248. [DOI] [PubMed] [Google Scholar]
  5. Bonen A., Tan M. H., Watson-Wright W. M. Insulin binding and glucose uptake differences in rodent skeletal muscles. Diabetes. 1981 Aug;30(8):702–704. doi: 10.2337/diab.30.8.702. [DOI] [PubMed] [Google Scholar]
  6. Carlisle H. J., Stock M. J. Potentiation of thermoregulatory responses to isoproterenol by beta-adrenergic antagonists. Am J Physiol. 1992 Oct;263(4 Pt 2):R915–R923. doi: 10.1152/ajpregu.1992.263.4.R915. [DOI] [PubMed] [Google Scholar]
  7. Carlisle H. J., Stock M. J. Thermoregulatory effects of beta adrenoceptors: effects of selective agonists and the interaction of antagonists with isoproterenol and BRL-35135 in the cold. J Pharmacol Exp Ther. 1993 Sep;266(3):1446–1453. [PubMed] [Google Scholar]
  8. Carlisle H. J., Stock M. J. Thermoregulatory responses to beta-adrenergic agonists at low ambient temperatures in the rat. Exp Physiol. 1993 Nov;78(6):775–786. doi: 10.1113/expphysiol.1993.sp003725. [DOI] [PubMed] [Google Scholar]
  9. Challiss R. A., Leighton B., Wilson S., Thurlby P. L., Arch J. R. An investigation of the beta-adrenoceptor that mediates metabolic responses to the novel agonist BRL28410 in rat soleus muscle. Biochem Pharmacol. 1988 Mar 1;37(5):947–950. doi: 10.1016/0006-2952(88)90186-4. [DOI] [PubMed] [Google Scholar]
  10. DeFronzo R. A., Ferrannini E., Hendler R., Felig P., Wahren J. Regulation of splanchnic and peripheral glucose uptake by insulin and hyperglycemia in man. Diabetes. 1983 Jan;32(1):35–45. doi: 10.2337/diab.32.1.35. [DOI] [PubMed] [Google Scholar]
  11. Emorine L. J., Marullo S., Briend-Sutren M. M., Patey G., Tate K., Delavier-Klutchko C., Strosberg A. D. Molecular characterization of the human beta 3-adrenergic receptor. Science. 1989 Sep 8;245(4922):1118–1121. doi: 10.1126/science.2570461. [DOI] [PubMed] [Google Scholar]
  12. Ferré P., Leturque A., Burnol A. F., Penicaud L., Girard J. A method to quantify glucose utilization in vivo in skeletal muscle and white adipose tissue of the anaesthetized rat. Biochem J. 1985 May 15;228(1):103–110. doi: 10.1042/bj2280103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Granneman J. G., Lahners K. N., Chaudhry A. Molecular cloning and expression of the rat beta 3-adrenergic receptor. Mol Pharmacol. 1991 Dec;40(6):895–899. [PubMed] [Google Scholar]
  14. Holness M. J., Sugden M. C. Glucose utilization in heart, diaphragm and skeletal muscle during the fed-to-starved transition. Biochem J. 1990 Aug 15;270(1):245–249. doi: 10.1042/bj2700245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Isler D., Hill H. P., Meier M. K. Glucose metabolism in isolated brown adipocytes under beta-adrenergic stimulation. Quantitative contribution of glucose to total thermogenesis. Biochem J. 1987 Aug 1;245(3):789–793. doi: 10.1042/bj2450789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Issad T., Pénicaud L., Ferré P., Kandé J., Baudon M. A., Girard J. Effects of fasting on tissue glucose utilization in conscious resting rats. Major glucose-sparing effect in working muscles. Biochem J. 1987 Aug 15;246(1):241–244. doi: 10.1042/bj2460241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. James D. E., Jenkins A. B., Kraegen E. W. Heterogeneity of insulin action in individual muscles in vivo: euglycemic clamp studies in rats. Am J Physiol. 1985 May;248(5 Pt 1):E567–E574. doi: 10.1152/ajpendo.1985.248.5.E567. [DOI] [PubMed] [Google Scholar]
  18. Krief S., Lönnqvist F., Raimbault S., Baude B., Van Spronsen A., Arner P., Strosberg A. D., Ricquier D., Emorine L. J. Tissue distribution of beta 3-adrenergic receptor mRNA in man. J Clin Invest. 1993 Jan;91(1):344–349. doi: 10.1172/JCI116191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Marette A., Bukowiecki L. J. Stimulation of glucose transport by insulin and norepinephrine in isolated rat brown adipocytes. Am J Physiol. 1989 Oct;257(4 Pt 1):C714–C721. doi: 10.1152/ajpcell.1989.257.4.C714. [DOI] [PubMed] [Google Scholar]
  20. Milligan G. Mechanisms of multifunctional signalling by G protein-linked receptors. Trends Pharmacol Sci. 1993 Jun;14(6):239–244. doi: 10.1016/0165-6147(93)90019-g. [DOI] [PubMed] [Google Scholar]
  21. Nahmias C., Blin N., Elalouf J. M., Mattei M. G., Strosberg A. D., Emorine L. J. Molecular characterization of the mouse beta 3-adrenergic receptor: relationship with the atypical receptor of adipocytes. EMBO J. 1991 Dec;10(12):3721–3727. doi: 10.1002/j.1460-2075.1991.tb04940.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Omatsu-Kanbe M., Kitasato H. Insulin and noradrenaline independently stimulate the translocation of glucose transporters from intracellular stores to the plasma membrane in mouse brown adipocytes. FEBS Lett. 1992 Dec 21;314(3):246–250. doi: 10.1016/0014-5793(92)81481-z. [DOI] [PubMed] [Google Scholar]
  23. Ploug T., Galbo H., Vinten J., Jørgensen M., Richter E. A. Kinetics of glucose transport in rat muscle: effects of insulin and contractions. Am J Physiol. 1987 Jul;253(1 Pt 1):E12–E20. doi: 10.1152/ajpendo.1987.253.1.E12. [DOI] [PubMed] [Google Scholar]
  24. Richter E. A., Garetto L. P., Goodman M. N., Ruderman N. B. Enhanced muscle glucose metabolism after exercise: modulation by local factors. Am J Physiol. 1984 Jun;246(6 Pt 1):E476–E482. doi: 10.1152/ajpendo.1984.246.6.E476. [DOI] [PubMed] [Google Scholar]
  25. Roberts S. J., Molenaar P., Summers R. J. Characterization of propranolol-resistant (-)-[125I]-cyanopindolol binding sites in rat soleus muscle. Br J Pharmacol. 1993 Jun;109(2):344–352. doi: 10.1111/j.1476-5381.1993.tb13576.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rochet N., Tanti J. F., Grémeaux T., Van Obberghen E., Le Marchand-Brustel Y. Effect of a thermogenic agent, BRL 26830A, on insulin receptors in obese mice. Am J Physiol. 1988 Aug;255(2 Pt 1):E101–E109. doi: 10.1152/ajpendo.1988.255.2.E101. [DOI] [PubMed] [Google Scholar]
  27. Sillence M. N., Moore N. G., Pegg G. G., Lindsay D. B. Ligand binding properties of putative beta 3-adrenoceptors compared in brown adipose tissue and in skeletal muscle membranes. Br J Pharmacol. 1993 Aug;109(4):1157–1163. doi: 10.1111/j.1476-5381.1993.tb13743.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Thurlby P. L., Ellis R. D. Differences between the effects of noradrenaline and the beta-adrenoceptor agonist BRL 28410 in brown adipose tissue and hind limb of the anaesthetized rat. Can J Physiol Pharmacol. 1986 Aug;64(8):1111–1114. doi: 10.1139/y86-189. [DOI] [PubMed] [Google Scholar]
  29. Wisneski J. A., Gertz E. W., Neese R. A., Gruenke L. D., Morris D. L., Craig J. C. Metabolic fate of extracted glucose in normal human myocardium. J Clin Invest. 1985 Nov;76(5):1819–1827. doi: 10.1172/JCI112174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Zaagsma J., Nahorski S. R. Is the adipocyte beta-adrenoceptor a prototype for the recently cloned atypical 'beta 3-adrenoceptor'? Trends Pharmacol Sci. 1990 Jan;11(1):3–7. doi: 10.1016/0165-6147(90)90032-4. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES