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. 1994 Dec;113(4):1328–1332. doi: 10.1111/j.1476-5381.1994.tb17143.x

The interaction of alpha-human atrial natriuretic peptide (ANP) with salbutamol, sodium nitroprusside and isosorbide dinitrate in human bronchial smooth muscle.

J E Nally 1, R A Clayton 1, N C Thomson 1, J C McGrath 1
PMCID: PMC1510526  PMID: 7889288

Abstract

1. Contractions in human bronchial rings evoked by methacholine (10(-6) M) were reversed by single contractions of alpha-human atrial natriuretic peptide (10(-6) M), salbutamol (10(-6) M), sodium nitroprusside (10(-6) M) or isosorbide dinitrate (4.2 x 10(-5) M) and the extent of the relaxations compared. The activity of combinations of ANP with salbutamol, sodium nitroprusside and isosorbide dinitrate were compared with those for each agonist alone. 2. ANP and salbutamol were equipotent in reversing methacholine-evoked contraction and, in combination these agonists evoked an additive response. ANP and sodium nitroprusside also evoked similar degrees of relaxation and were additive, as were ANP and isosorbide dinitrate; however, with isosorbide dinitrate a higher concentration was required to evoke the same degree of relaxation as ANP, sodium nitroprusside or salbutamol. 3. Cumulative concentration-response curves to methacholine (10(-9)-3 x 10(-4) M) were examined in the presence and absence of the above bronchodilator substances, alone and in combination allowing their abilities to protect against contraction to be compared. ANP (10(-6) M) and salbutamol (10(-6) M) each attenuated subsequent contractions evoked by methacholine, an ability not shared with sodium nitroprusside (10(-6) M) or isosorbide dinitrate (4.2 x 10(-5) M). Indeed at lower concentrations of methacholine (< 3 x 10(-7) M), sodium nitroprusside evoked a paradoxical enhancement of methacholine-evoked contractions. 4. In combination, ANP and salbutamol attenuated contractions evoked by methacholine to a significantly greater degree than that seen with either agonist alone, whilst a combination of ANP and sodium nitroprusside evoked no greater effect than that seen with ANP alone.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Advenier C., Candenas M. L., Naline E., De Vos C. The effect of cetirizine on the human isolated bronchus: interaction with salbutamol. J Allergy Clin Immunol. 1991 Jul;88(1):104–113. doi: 10.1016/0091-6749(91)90307-a. [DOI] [PubMed] [Google Scholar]
  2. Advenier C., Naline E., Matran R., Toty L., Bakdach H. Interaction between fenoterol, ipratropium, and acetylcholine on human isolated bronchus. J Allergy Clin Immunol. 1988 Jul;82(1):40–46. doi: 10.1016/0091-6749(88)90049-8. [DOI] [PubMed] [Google Scholar]
  3. Anderson J., Struthers A., Christofides N., Bloom S. Atrial natriuretic peptide: an endogenous factor enhancing sodium excretion in man. Clin Sci (Lond) 1986 Apr;70(4):327–331. doi: 10.1042/cs0700327. [DOI] [PubMed] [Google Scholar]
  4. Angus R. M., McCallum M. J., Hulks G., Thomson N. C. Bronchodilator, cardiovascular, and cyclic guanylyl monophosphate response to high-dose infused atrial natriuretic peptide in asthma. Am Rev Respir Dis. 1993 May;147(5):1122–1125. doi: 10.1164/ajrccm/147.5.1122. [DOI] [PubMed] [Google Scholar]
  5. Angus R. M., Nally J. E., McCall R., Young L. C., McGrath J. C., Thomson N. C. Modulation of the effect of atrial natriuretic peptide in human and bovine bronchi by phosphoramidon. Clin Sci (Lond) 1994 Mar;86(3):291–295. doi: 10.1042/cs0860291. [DOI] [PubMed] [Google Scholar]
  6. Brink C., Grimaud C., Guillot C., Orehek J. The interaction between indomethacin and contractile agents on human isolated airway muscle. Br J Pharmacol. 1980 Jul;69(3):383–388. doi: 10.1111/j.1476-5381.1980.tb07026.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Britton J., Hanley S. P., Garrett H. V., Hadfield J. W., Tattersfield A. E. Dose related effects of salbutamol and ipratropium bromide on airway calibre and reactivity in subjects with asthma. Thorax. 1988 Apr;43(4):300–305. doi: 10.1136/thx.43.4.300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Feelisch M., Noack E. A. Correlation between nitric oxide formation during degradation of organic nitrates and activation of guanylate cyclase. Eur J Pharmacol. 1987 Jul 2;139(1):19–30. doi: 10.1016/0014-2999(87)90493-6. [DOI] [PubMed] [Google Scholar]
  9. Gruetter C. A., Childers C. E., Bosserman M. K., Lemke S. M., Ball J. G., Valentovic M. A. Comparison of relaxation induced by glyceryl trinitrate, isosorbide dinitrate, and sodium nitroprusside in bovine airways. Am Rev Respir Dis. 1989 May;139(5):1192–1197. doi: 10.1164/ajrccm/139.5.1192. [DOI] [PubMed] [Google Scholar]
  10. Gustafsson B., Persson C. G. Effect of different bronchodilators on airway smooth muscle responsiveness to contractile agents. Thorax. 1991 May;46(5):360–365. doi: 10.1136/thx.46.5.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gutkowska J., Nemer M. Structure, expression, and function of atrial natriuretic factor in extraatrial tissues. Endocr Rev. 1989 Nov;10(4):519–536. doi: 10.1210/edrv-10-4-519. [DOI] [PubMed] [Google Scholar]
  12. Hulks G., Jardine A. G., Connell J. M., Thomson N. C. Influence of elevated plasma levels of atrial natriuretic factor on bronchial reactivity in asthma. Am Rev Respir Dis. 1991 Apr;143(4 Pt 1):778–782. doi: 10.1164/ajrccm/143.4_Pt_1.778. [DOI] [PubMed] [Google Scholar]
  13. Hulks G., Jardine A., Connell J. M., Thomson N. C. Bronchodilator effect of atrial natriuretic peptide in asthma. BMJ. 1989 Oct 28;299(6707):1081–1082. doi: 10.1136/bmj.299.6707.1081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hulks G., Thomson N. C. Inhaled atrial natriuretic peptide and asthmatic airways. BMJ. 1992 May 2;304(6835):1156–1156. doi: 10.1136/bmj.304.6835.1156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ignarro L. J., Lippton H., Edwards J. C., Baricos W. H., Hyman A. L., Kadowitz P. J., Gruetter C. A. Mechanism of vascular smooth muscle relaxation by organic nitrates, nitrites, nitroprusside and nitric oxide: evidence for the involvement of S-nitrosothiols as active intermediates. J Pharmacol Exp Ther. 1981 Sep;218(3):739–749. [PubMed] [Google Scholar]
  16. Ishii K., Murad F. ANP relaxes bovine tracheal smooth muscle and increases cGMP. Am J Physiol. 1989 Mar;256(3 Pt 1):C495–C500. doi: 10.1152/ajpcell.1989.256.3.C495. [DOI] [PubMed] [Google Scholar]
  17. Jang E. K., Davidson M. M., Crankshaw D., Haslam R. J. Synergistic inhibitory effects of atriopeptin II and isoproterenol on contraction of rat aortic smooth muscle: roles of cGMP and cAMP. Eur J Pharmacol. 1993 Dec 21;250(3):477–481. doi: 10.1016/0014-2999(93)90038-j. [DOI] [PubMed] [Google Scholar]
  18. Karlsson J. A., Persson C. G. Influence of tracheal contraction on relaxant effects in vitro of theophylline and isoprenaline. Br J Pharmacol. 1981 Sep;74(1):73–79. doi: 10.1111/j.1476-5381.1981.tb09956.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Maurice D. H., Crankshaw D., Haslam R. J. Synergistic actions of nitrovasodilators and isoprenaline on rat aortic smooth muscle. Eur J Pharmacol. 1991 Jan 10;192(2):235–242. doi: 10.1016/0014-2999(91)90048-u. [DOI] [PubMed] [Google Scholar]
  20. Mcalpine L. G., Hulks G., Thomson N. C. Effect of atrial natriuretic peptide given by intravenous infusion on bronchoconstriction induced by ultrasonically nebulized distilled water (fog). Am Rev Respir Dis. 1992 Oct;146(4):912–915. doi: 10.1164/ajrccm/146.4.912. [DOI] [PubMed] [Google Scholar]
  21. Miura M., Belvisi M. G., Stretton C. D., Yacoub M. H., Barnes P. J. Role of potassium channels in bronchodilator responses in human airways. Am Rev Respir Dis. 1992 Jul;146(1):132–136. doi: 10.1164/ajrccm/146.1.132. [DOI] [PubMed] [Google Scholar]
  22. Persson C. G., Erjefält I., Grega G. J., Svensjö E. The role of beta-receptor agonists in the inhibition of pulmonary edema. Ann N Y Acad Sci. 1982;384:544–557. doi: 10.1111/j.1749-6632.1982.tb21399.x. [DOI] [PubMed] [Google Scholar]
  23. Rinard G. A., Jensen A., Puckett A. M. Hydrocortisone and isoproterenol effects on trachealis cAMP and relaxation. J Appl Physiol Respir Environ Exerc Physiol. 1983 Nov;55(5):1609–1613. doi: 10.1152/jappl.1983.55.5.1609. [DOI] [PubMed] [Google Scholar]
  24. Sagnella G. A., MacGregor G. A. Physiology: cardiac peptides and the control of sodium excretion. Nature. 1984 Jun 21;309(5970):666–667. doi: 10.1038/309666a0. [DOI] [PubMed] [Google Scholar]
  25. Tattersfield A. E. Effect of beta-agonists and anticholinergic drugs on bronchial reactivity. Am Rev Respir Dis. 1987 Oct;136(4 Pt 2):S64–S68. doi: 10.1164/ajrccm/136.4_Pt_2.S64. [DOI] [PubMed] [Google Scholar]
  26. Tullett W. M., Patel K. R. Isosorbide dinitrate and isoxsuprine in exercise induced asthma. Br Med J (Clin Res Ed) 1983 Jun 18;286(6382):1934–1935. doi: 10.1136/bmj.286.6382.1934-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. van den Brink F. G. The model of functional interaction. II. Experimental verification of a new model: the antagonism of beta-adrenoceptor stimulants and other agonists. Eur J Pharmacol. 1973 Jun;22(3):279–286. doi: 10.1016/0014-2999(73)90027-7. [DOI] [PubMed] [Google Scholar]

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