Skip to main content
NCBI home page
Archives of Disease in Childhood logoLink to Archives of Disease in Childhood
. 1996 Aug;75(2):133–136. doi: 10.1136/adc.75.2.133

Surfactant abnormalities in infants with severe viral bronchiolitis.

P A Dargaville 1, M South 1, P N McDougall 1
PMCID: PMC1511649  PMID: 8869194

Abstract

To determine whether abnormalities of pulmonary surfactant occur in infants with acute viral bronchiolitis, surfactant indices were measured in lung lavage fluid from 12 infants with severe bronchiolitis and eight infants without lung disease. Compared with controls, the bronchiolitis group showed deficiency of surfactant protein A (1.02 v 14.4 micrograms/ml) and disaturated phosphatidylcholine (35 v 1060 micrograms/ml) which resolved as the disease improved. Surfactant functional activity was also impaired (minimum surface tension 22 v 17 mN/m). These findings indicate that surfactant abnormalities occur in bronchiolitis, and may represent one of the pathophysiological mechanisms causing airway obstruction.

Full text

PDF
133

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auten R. L., Watkins R. H., Shapiro D. L., Horowitz S. Surfactant apoprotein A (SP-A) is synthesized in airway cells. Am J Respir Cell Mol Biol. 1990 Nov;3(5):491–496. doi: 10.1165/ajrcmb/3.5.491. [DOI] [PubMed] [Google Scholar]
  2. Bui K. C., Walther F. J., David-Cu R., Garg M., Warburton D. Phospholipid and surfactant protein A concentrations in tracheal aspirates from infants requiring extracorporeal membrane oxygenation. J Pediatr. 1992 Aug;121(2):271–274. doi: 10.1016/s0022-3476(05)81202-8. [DOI] [PubMed] [Google Scholar]
  3. Cockshutt A. M., Weitz J., Possmayer F. Pulmonary surfactant-associated protein A enhances the surface activity of lipid extract surfactant and reverses inhibition by blood proteins in vitro. Biochemistry. 1990 Sep 11;29(36):8424–8429. doi: 10.1021/bi00488a032. [DOI] [PubMed] [Google Scholar]
  4. Enhorning G., Duffy L. C., Welliver R. C. Pulmonary surfactant maintains patency of conducting airways in the rat. Am J Respir Crit Care Med. 1995 Feb;151(2 Pt 1):554–556. doi: 10.1164/ajrccm.151.2.7842219. [DOI] [PubMed] [Google Scholar]
  5. Enhorning G., Holm B. A. Disruption of pulmonary surfactant's ability to maintain openness of a narrow tube. J Appl Physiol (1985) 1993 Jun;74(6):2922–2927. doi: 10.1152/jappl.1993.74.6.2922. [DOI] [PubMed] [Google Scholar]
  6. Enhorning G. Pulsating bubble technique for evaluating pulmonary surfactant. J Appl Physiol Respir Environ Exerc Physiol. 1977 Aug;43(2):198–203. doi: 10.1152/jappl.1977.43.2.198. [DOI] [PubMed] [Google Scholar]
  7. Gil J., Weibel E. R. Extracellular lining of bronchioles after perfusion-fixation of rat lungs for electron microscopy. Anat Rec. 1971 Feb;169(2):185–199. doi: 10.1002/ar.1091690205. [DOI] [PubMed] [Google Scholar]
  8. Hallman M., Merritt T. A., Akino T., Bry K. Surfactant protein A, phosphatidylcholine, and surfactant inhibitors in epithelial lining fluid. Correlation with surface activity, severity of respiratory distress syndrome, and outcome in small premature infants. Am Rev Respir Dis. 1991 Dec;144(6):1376–1384. doi: 10.1164/ajrccm/144.6.1376. [DOI] [PubMed] [Google Scholar]
  9. Halpern D., Grotberg J. B. Surfactant effects on fluid-elastic instabilities of liquid-lined flexible tubes: a model of airway closure. J Biomech Eng. 1993 Aug;115(3):271–277. doi: 10.1115/1.2895486. [DOI] [PubMed] [Google Scholar]
  10. Kuroki Y., Takahashi H., Fukada Y., Mikawa M., Inagawa A., Fujimoto S., Akino T. Two-site "simultaneous" immunoassay with monoclonal antibodies for the determination of surfactant apoproteins in human amniotic fluid. Pediatr Res. 1985 Oct;19(10):1017–1020. doi: 10.1203/00006450-198510000-00013. [DOI] [PubMed] [Google Scholar]
  11. Mason R. J., Nellenbogen J., Clements J. A. Isolation of disaturated phosphatidylcholine with osmium tetroxide. J Lipid Res. 1976 May;17(3):281–284. [PubMed] [Google Scholar]
  12. REYNOLDS E. O., COOK C. D. THE TREATMENT OF BRONCHIOLITIS. J Pediatr. 1963 Dec;63:1205–1207. doi: 10.1016/s0022-3476(63)80215-2. [DOI] [PubMed] [Google Scholar]
  13. Rakshi K., Couriel J. M. Management of acute bronchiolitis. Arch Dis Child. 1994 Nov;71(5):463–469. doi: 10.1136/adc.71.5.463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rennard S. I., Basset G., Lecossier D., O'Donnell K. M., Pinkston P., Martin P. G., Crystal R. G. Estimation of volume of epithelial lining fluid recovered by lavage using urea as marker of dilution. J Appl Physiol (1985) 1986 Feb;60(2):532–538. doi: 10.1152/jappl.1986.60.2.532. [DOI] [PubMed] [Google Scholar]
  15. Rubin B. K., Ramirez O., King M. Mucus rheology and transport in neonatal respiratory distress syndrome and the effect of surfactant therapy. Chest. 1992 Apr;101(4):1080–1085. doi: 10.1378/chest.101.4.1080. [DOI] [PubMed] [Google Scholar]
  16. Venkitaraman A. R., Baatz J. E., Whitsett J. A., Hall S. B., Notter R. H. Biophysical inhibition of synthetic phospholipid-lung surfactant apoprotein admixtures by plasma proteins. Chem Phys Lipids. 1991 Jan-Feb;57(1):49–57. doi: 10.1016/0009-3084(91)90048-g. [DOI] [PubMed] [Google Scholar]
  17. Walker S. R., Williams M. C., Benson B. Immunocytochemical localization of the major surfactant apoproteins in type II cells, Clara cells, and alveolar macrophages of rat lung. J Histochem Cytochem. 1986 Sep;34(9):1137–1148. doi: 10.1177/34.9.2426341. [DOI] [PubMed] [Google Scholar]

Articles from Archives of Disease in Childhood are provided here courtesy of BMJ Publishing Group

RESOURCES