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. 1995 Oct;103(Suppl 7):21–22. doi: 10.1289/ehp.95103s721

Effects of estrogens on microtubule polymerization in vitro: correlation with estrogenicity.

M Metzler 1, E Pfeiffer 1
PMCID: PMC1518868  PMID: 8593868

Abstract

Several estrogens of diverse classes, namely stilbene estrogens, steroidal estrogens, phytoestrogens, mycoestrogens, lignans, and bisphenol A, were studied for their ability to interfere with the assembly of microtubules under cell-free conditions. Inhibition of microtubules in intact cells may lead to the induction of micronuclei and aneuploidy and thereby contribute to estrogen-mediated carcinogenesis. Diethylstilbestrol and various other stilbene estrogens, as well as bisphenol A, were good inhibitors of microtubule polymerization whereas the other tested compounds were devoid of this activity. Therefore, the ability of estrogenic substances to interact with microtubules does not correlate with the hormonal activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrett J. C., Wong A., McLachlan J. A. Diethylstilbestrol induces neoplastic transformation without measurable gene mutation at two loci. Science. 1981 Jun 19;212(4501):1402–1404. doi: 10.1126/science.6262919. [DOI] [PubMed] [Google Scholar]
  2. Degen G. H., Metzler M. Sex hormones and neoplasia: genotoxic effects in short term assays. Arch Toxicol Suppl. 1987;10:264–278. doi: 10.1007/978-3-642-71617-1_24. [DOI] [PubMed] [Google Scholar]
  3. Epe B., Harttig U., Stopper H., Metzler M. Covalent binding of reactive estrogen metabolites to microtubular protein as a possible mechanism of aneuploidy induction and neoplastic cell transformation. Environ Health Perspect. 1990 Aug;88:123–127. doi: 10.1289/ehp.9088123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hartley-Asp B., Deinum J., Wallin M. Diethylstilbestrol induces metaphase arrest and inhibits microtubule assembly. Mutat Res. 1985 Aug;143(4):231–235. doi: 10.1016/0165-7992(85)90086-7. [DOI] [PubMed] [Google Scholar]
  5. Korach K. S., Metzler M., McLachlan J. A. Estrogenic activity in vivo and in vitro of some diethylstilbestrol metabolites and analogs. Proc Natl Acad Sci U S A. 1978 Jan;75(1):468–471. doi: 10.1073/pnas.75.1.468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Li J. J., Li S. A., Klicka J. K., Parsons J. A., Lam L. K. Relative carcinogenic activity of various synthetic and natural estrogens in the Syrian hamster kidney. Cancer Res. 1983 Nov;43(11):5200–5204. [PubMed] [Google Scholar]
  7. McLachlan J. A., Wong A., Degen G. H., Barrett J. C. Morphologic and neoplastic transformation of Syrian hamster embryo fibroblasts by diethylstilbestrol and its analogs. Cancer Res. 1982 Aug;42(8):3040–3045. [PubMed] [Google Scholar]
  8. Ozawa N., Oshimura M., McLachlan J. A., Barrett J. C. Nonrandom karyotypic changes in immortal and tumorigenic Syrian hamster cells induced by diethylstilbestrol. Cancer Genet Cytogenet. 1989 Apr;38(2):271–282. doi: 10.1016/0165-4608(89)90669-9. [DOI] [PubMed] [Google Scholar]
  9. Parry E. M., Danford N., Parry J. M. Differential staining of chromosomes and spindle and its use as an assay for determining the effect of diethylstilboestrol on cultured mammalian cells. Mutat Res. 1982 Oct;105(4):243–252. doi: 10.1016/0165-7992(82)90037-9. [DOI] [PubMed] [Google Scholar]
  10. Sakakibara Y., Saito I., Ichinoseki K., Oda T., Kaneko M., Saitô H., Kodama M., Sato Y. Effects of diethylstilbestrol and its methyl ethers on aneuploidy induction and microtubule distribution in Chinese hamster V79 cells. Mutat Res. 1991 Aug;263(4):269–276. doi: 10.1016/0165-7992(91)90012-s. [DOI] [PubMed] [Google Scholar]
  11. Sato Y., Murai T., Tsumuraya M., Saitô H., Kodama M. Disruptive effect of diethylstilbestrol on microtubules. Gan. 1984 Dec;75(12):1046–1048. [PubMed] [Google Scholar]
  12. Sawada M., Ishidate M., Jr Colchicine-like effect of diethylstilbestrol (DES) on mammalian cells in vitro. Mutat Res. 1978 May;57(2):175–182. doi: 10.1016/0027-5107(78)90265-8. [DOI] [PubMed] [Google Scholar]
  13. Sharp D. C., Parry J. M. Diethylstilboestrol: the binding and effects of diethylstilboestrol upon the polymerisation and depolymerisation of purified microtubule protein in vitro. Carcinogenesis. 1985 Jun;6(6):865–871. doi: 10.1093/carcin/6.6.865. [DOI] [PubMed] [Google Scholar]
  14. Tsutsui T., Maizumi H., McLachlan J. A., Barrett J. C. Aneuploidy induction and cell transformation by diethylstilbestrol: a possible chromosomal mechanism in carcinogenesis. Cancer Res. 1983 Aug;43(8):3814–3821. [PubMed] [Google Scholar]
  15. Tsutsui T., Suzuki N., Fukuda S., Sato M., Maizumi H., McLachlan J. A., Barrett J. C. 17beta-Estradiol-induced cell transformation and aneuploidy of Syrian hamster embryo cells in culture. Carcinogenesis. 1987 Nov;8(11):1715–1719. doi: 10.1093/carcin/8.11.1715. [DOI] [PubMed] [Google Scholar]
  16. Tucker R. W., Barrett J. C. Deceased numbers of spindle and cytoplasmic microtubules in hamster embryo cells treated with a carcinogen, diethylstilbestrol. Cancer Res. 1986 Apr;46(4 Pt 2):2088–2095. [PubMed] [Google Scholar]
  17. Wheeler W. J., Cherry L. M., Downs T., Hsu T. C. Mitotic inhibition and aneuploidy induction by naturally occurring and synthetic estrogens in Chinese hamster cells in vitro. Mutat Res. 1986 Jul;171(1):31–41. doi: 10.1016/0165-1218(86)90006-6. [DOI] [PubMed] [Google Scholar]

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