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. 1995 May;103(Suppl 4):67–72. doi: 10.1289/ehp.95103s467

Decreased lymphocyte responses in free-ranging bottlenose dolphins (Tursiops truncatus) are associated with increased concentrations of PCBs and DDT in peripheral blood.

G P Lahvis 1, R S Wells 1, D W Kuehl 1, J L Stewart 1, H L Rhinehart 1, C S Via 1
PMCID: PMC1519269  PMID: 7556026

Abstract

Since 1987, large-scale mortalities of dolphins have been reported along the Atlantic coast of North America, in the Gulf of Mexico, and in the Mediterranean Sea. Autopsied bottlenose dolphins, Tursiops truncatus, which were collected from the large-scale mortality along the Atlantic coast in 1987 to 1988, exhibited opportunistic infections indicative of immune dysfunction. Further, these animals had high levels of chlorinated hydrocarbons, such as PCBs and DDT, that can suppress immune functions. The purpose of this study was to determine whether there is a relationship between chemical contaminant exposure and immune response in free-ranging dolphins. In June of 1991, peripheral blood was obtained from members of a bottlenose dolphin population that resides along the west coast of Florida. Peripheral blood lymphocyte responses to Concanavalin A (Con A) and phytohemagglutinin (PHA) were determined in vitro and compared by regression analysis with contaminant concentrations in whole blood from a small subset of these animals (n = 5). These data indicate that a reduced immune response in these bottlenose dolphins was correlated with increasing whole blood concentrations of several contaminants. Specifically, inverse correlations were found between Con A-induced lymphocyte proliferation and tetrachlorinated to octachlorinated biphenyls (r2 values ranged from 0.70 to 0.87). Con A-induced lymphocyte responses also correlated inversely with p,p'DDT (r2 values of 0.73 and 0.79); o.p'-DDE (r2 values of 0.93 and 0.96); and p,p'-DDE (r2 values of 0.73 and 0.81).

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Selected References

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  1. Banerjee B. D. Effects of sub-chronic DDT exposure on humoral and cell-mediated immune responses in albino rats. Bull Environ Contam Toxicol. 1987 Nov;39(5):827–834. doi: 10.1007/BF01855862. [DOI] [PubMed] [Google Scholar]
  2. Banerjee B. D., Ramachandran M., Hussain Q. Z. Sub-chronic effect of DDT on humoral immune response in mice. Bull Environ Contam Toxicol. 1986 Sep;37(3):433–440. doi: 10.1007/BF01607785. [DOI] [PubMed] [Google Scholar]
  3. Banerjee B. D. Sub-chronic effect of DDT on humoral immune response to a thymus-independent antigen (bacterial lipopolysaccharide) in mice. Bull Environ Contam Toxicol. 1987 Nov;39(5):822–826. doi: 10.1007/BF01855861. [DOI] [PubMed] [Google Scholar]
  4. Chang K. J., Hsieh K. H., Tang S. Y., Tung T. C., Lee T. P. Immunologic evaluation of patients with polychlorinated biphenyl poisoning: evaluation of delayed-type skin hypersensitive response and its relation to clinical studies. J Toxicol Environ Health. 1982 Feb;9(2):217–223. doi: 10.1080/15287398209530156. [DOI] [PubMed] [Google Scholar]
  5. Colgrove G. S. Stimulation of lymphocytes from a dolphin (Tursiops truncatus) by phytomitogens. Am J Vet Res. 1978 Jan;39(1):141–144. [PubMed] [Google Scholar]
  6. Domingo M., Visa J., Pumarola M., Marco A. J., Ferrer L., Rabanal R., Kennedy S. Pathologic and immunocytochemical studies of morbillivirus infection in striped dolphins (Stenella coeruleoalba). Vet Pathol. 1992 Jan;29(1):1–10. doi: 10.1177/030098589202900101. [DOI] [PubMed] [Google Scholar]
  7. Friend M., Trainer D. O. Polychlorinated biphenyl: interaction with duck hepatitis virus. Science. 1970 Dec 18;170(3964):1314–1316. doi: 10.1126/science.170.3964.1314. [DOI] [PubMed] [Google Scholar]
  8. Imanishi J., Nomura H., Matsubara M., Kita M., Won S. J., Mizutani T., Kishida T. Effect of polychlorinated biphenyl on viral infections in mice. Infect Immun. 1980 Jul;29(1):275–277. doi: 10.1128/iai.29.1.275-277.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kannan K., Tanabe S., Borrell A., Aguilar A., Focardi S., Tatsukawa R. Isomer-specific analysis and toxic evaluation of polychlorinated biphenyls in striped dolphins affected by an epizootic in the western Mediterranean sea. Arch Environ Contam Toxicol. 1993 Aug;25(2):227–233. doi: 10.1007/BF00212134. [DOI] [PubMed] [Google Scholar]
  10. Kennedy S., Smyth J. A., Cush P. F., McCullough S. J., Allan G. M., McQuaid S. Viral distemper now found in porpoises. Nature. 1988 Nov 3;336(6194):21–21. doi: 10.1038/336021a0. [DOI] [PubMed] [Google Scholar]
  11. Kerkvliet N. I., Baecher-Steppan L., Smith B. B., Youngberg J. A., Henderson M. C., Buhler D. R. Role of the Ah locus in suppression of cytotoxic T lymphocyte activity by halogenated aromatic hydrocarbons (PCBs and TCDD): structure-activity relationships and effects in C57Bl/6 mice congenic at the Ah locus. Fundam Appl Toxicol. 1990 Apr;14(3):532–541. doi: 10.1016/0272-0590(90)90257-k. [DOI] [PubMed] [Google Scholar]
  12. Lipscomb T. P., Kennedy S., Moffett D., Ford B. K. Morbilliviral disease in an Atlantic bottlenose dolphin (Tursiops truncatus) from the Gulf of Mexico. J Wildl Dis. 1994 Oct;30(4):572–576. doi: 10.7589/0090-3558-30.4.572. [DOI] [PubMed] [Google Scholar]
  13. Lipscomb T. P., Schulman F. Y., Moffett D., Kennedy S. Morbilliviral disease in Atlantic bottlenose dolphins (Tursiops truncatus) from the 1987-1988 epizootic. J Wildl Dis. 1994 Oct;30(4):567–571. doi: 10.7589/0090-3558-30.4.567. [DOI] [PubMed] [Google Scholar]
  14. Loose L. D., Silkworth J. B., Pittman K. A., Benitz K. F., Mueller W. Impaired host resistance to endotoxin and malaria in polychlorinated biphenyl- and hexachlorobenzene-treated mice. Infect Immun. 1978 Apr;20(1):30–35. doi: 10.1128/iai.20.1.30-35.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Martineau D., Lagacé A., Béland P., Higgins R., Armstrong D., Shugart L. R. Pathology of stranded beluga whales (Delphinapterus leucas) from the St. Lawrence Estuary, Québec, Canada. J Comp Pathol. 1988 Apr;98(3):287–311. doi: 10.1016/0021-9975(88)90038-2. [DOI] [PubMed] [Google Scholar]
  16. Massé R., Martineau D., Tremblay L., Béland P. Concentrations and chromatographic profile of DDT metabolites and polychlorobiphenyl (PCB) residues in stranded beluga whales (Delphinapterus leucas) from the St. Lawrence Estuary, Canada. Arch Environ Contam Toxicol. 1986 Sep;15(5):567–579. doi: 10.1007/BF01056571. [DOI] [PubMed] [Google Scholar]
  17. Muir D. C., Wagemann R., Grift N. P., Norstrom R. J., Simon M., Lien J. Organochlorine chemical and heavy metal contaminants in white-beaked dolphins (Lagenorhynchus albirostris) and pilot whales (Globicephala melaena) from the coast of Newfoundland, Canada. Arch Environ Contam Toxicol. 1988 Sep;17(5):613–629. doi: 10.1007/BF01055830. [DOI] [PubMed] [Google Scholar]
  18. Mumford D. M., Stockman G. D., Barsales P. B., Whitman T., Wilbur J. R. Lymphocyte transformation studies of sea mammal blood. Experientia. 1975 Apr 15;31(4):498–500. doi: 10.1007/BF02026402. [DOI] [PubMed] [Google Scholar]
  19. Silkworth J. B., Antrim L., Kaminsky L. S. Correlations between polychlorinated biphenyl immunotoxicity, the aromatic hydrocarbon locus, and liver microsomal enzyme induction in C57BL/6 and DBA/2 mice. Toxicol Appl Pharmacol. 1984 Aug;75(1):156–165. doi: 10.1016/0041-008x(84)90086-3. [DOI] [PubMed] [Google Scholar]
  20. Silkworth J. B., Grabstein E. M. Polychlorinated biphenyl immunotoxicity: dependence on isomer planarity and the Ah gene complex. Toxicol Appl Pharmacol. 1982 Aug;65(1):109–115. doi: 10.1016/0041-008x(82)90368-4. [DOI] [PubMed] [Google Scholar]
  21. Smialowicz R. J., Andrews J. E., Riddle M. M., Rogers R. R., Luebke R. W., Copeland C. B. Evaluation of the immunotoxicity of low level PCB exposure in the rat. Toxicology. 1989 Jun 1;56(2):197–211. doi: 10.1016/0300-483x(89)90133-9. [DOI] [PubMed] [Google Scholar]
  22. Thomas P. T., Hinsdill R. D. Effect of polychlorinated biphenyls on the immune responses of rhesus monkeys and mice. Toxicol Appl Pharmacol. 1978 Apr;44(1):41–51. doi: 10.1016/0041-008x(78)90282-x. [DOI] [PubMed] [Google Scholar]
  23. Tryphonas H., Luster M. I., Schiffman G., Dawson L. L., Hodgen M., Germolec D., Hayward S., Bryce F., Loo J. C., Mandy F. Effect of chronic exposure of PCB (Aroclor 1254) on specific and nonspecific immune parameters in the rhesus (Macaca mulatta) monkey. Fundam Appl Toxicol. 1991 May;16(4):773–786. doi: 10.1016/0272-0590(91)90163-x. [DOI] [PubMed] [Google Scholar]
  24. Tryphonas H., Luster M. I., White K. L., Jr, Naylor P. H., Erdos M. R., Burleson G. R., Germolec D., Hodgen M., Hayward S., Arnold D. L. Effects of PCB (Aroclor 1254) on non-specific immune parameters in rhesus (Macaca mulatta) monkeys. Int J Immunopharmacol. 1991;13(6):639–648. doi: 10.1016/0192-0561(91)90176-8. [DOI] [PubMed] [Google Scholar]
  25. Vos J. G., de Roij T. Immunosuppressive activity of a polychlorinated diphenyl preparation on the humoral immune response in guinea pigs. Toxicol Appl Pharmacol. 1972 Apr;21(4):549–555. doi: 10.1016/0041-008x(72)90011-7. [DOI] [PubMed] [Google Scholar]
  26. Wassermann M., Wassermann D., Gershon A., Zellermayer L. Effects of organochlorine insecti-c cides on body defense systems. Ann N Y Acad Sci. 1969;160(1):393–401. doi: 10.1111/j.1749-6632.1969.tb15861.x. [DOI] [PubMed] [Google Scholar]

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