Abstract
The bronchoalveolar leukocytes from quartz-inflamed lung were separated into macrophage-enriched and neutrophil-enriched populations on density gradients. Neutrophil-enriched populations showed the greatest activity in causing injury to epithelial cells and fibronectin in vitro. Inflammatory macrophage-enriched populations from quartz-exposed lung had the ability to cause fibronectin degradation but could not cause detachment injury to epithelial cells over and above that caused by control alveolar macrophages. Fibronectin damage in vivo could be an important factor in disordering the connective tissue scaffold of the lung, thereby favoring fibrosis. In vitro quartz stimulated more production of cytokines by alveolar macrophages than the inert particulate titanium dioxide. Cytokines could be important in upregulating adhesion molecules in the membranes of lung cells in vivo; this process could aid leukocyte/lung cell contact, allowing epithelial injury to be expressed, and could also be a factor leading to pathological change.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Albelda S. M., Buck C. A. Integrins and other cell adhesion molecules. FASEB J. 1990 Aug;4(11):2868–2880. [PubMed] [Google Scholar]
- Brown G. M., Donaldson K., Brown D. M. Bronchoalveolar leukocyte response in experimental silicosis: modulation by a soluble aluminum compound. Toxicol Appl Pharmacol. 1989 Oct;101(1):95–105. doi: 10.1016/0041-008x(89)90215-9. [DOI] [PubMed] [Google Scholar]
- Brown G. M., Donaldson K. Degradation of connective tissue components by lung derived leucocytes in vitro: role of proteases and oxidants. Thorax. 1988 Feb;43(2):132–139. doi: 10.1136/thx.43.2.132. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donaldson K., Bolton R. E., Jones A., Brown G. M., Robertson M. D., Slight J., Cowie H., Davis J. M. Kinetics of the bronchoalveolar leucocyte response in rats during exposure to equal airborne mass concentrations of quartz, chrysotile asbestos, or titanium dioxide. Thorax. 1988 Jul;43(7):525–533. doi: 10.1136/thx.43.7.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donaldson K., Brown G. M., Brown D. M., Robertson M. D., Slight J., Cowie H., Jones A. D., Bolton R. E., Davis J. M. Contrasting bronchoalveolar leukocyte responses in rats inhaling coal mine dust, quartz, or titanium dioxide: effects of coal rank, airborne mass concentration, and cessation of exposure. Environ Res. 1990 Jun;52(1):62–76. doi: 10.1016/s0013-9351(05)80151-3. [DOI] [PubMed] [Google Scholar]
- Donaldson K., Slight J., Brown G. M., Bolton R. E. The ability of inflammatory bronchoalveolar leucocyte populations elicited with microbes or mineral dust to injure alveolar epithelial cells and degrade extracellular matrix in vitro. Br J Exp Pathol. 1988 Jun;69(3):327–338. [PMC free article] [PubMed] [Google Scholar]
- Kusaka Y., Donaldson K. Production of interleukin-1 like activity by neutrophils derived from rat lung. Thorax. 1990 Apr;45(4):281–282. doi: 10.1136/thx.45.4.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Osborn L., Hession C., Tizard R., Vassallo C., Luhowskyj S., Chi-Rosso G., Lobb R. Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytes. Cell. 1989 Dec 22;59(6):1203–1211. doi: 10.1016/0092-8674(89)90775-7. [DOI] [PubMed] [Google Scholar]