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. 1992 Nov;98:75–80. doi: 10.1289/ehp.929875

Elucidation of catalytic specificities of human cytochrome P450 and glutathione S-transferase enzymes and relevance to molecular epidemiology.

F P Guengerich 1, T Shimada 1, K D Raney 1, C H Yun 1, D J Meyer 1, B Ketterer 1, T M Harris 1, J D Groopman 1, F F Kadlubar 1
PMCID: PMC1519604  PMID: 1486866

Abstract

A number of different approaches have been used to determine the catalytic selectivity of individual human enzymes toward procarcinogens. Studies with cytochrome P450 (P450) enzymes and glutathione S-transferases are summarized here, and recent work with pyrrolizidine alkaloids, aflatoxins, 4,4'-methylenebis(2-chloroaniline), and ethyl carbamate is discussed. In some cases a single enzyme can catalyze both activation and detoxication reactions of a chemical. The purification and characterization of human lung P4501A1 and the development of a noninvasive assay for human P4502E1 are also described.

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Selected References

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