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. 1993 Oct;101(Suppl 3):11–13. doi: 10.1289/ehp.101-1521140

Using the micronucleus assay to detect genotoxic effects of metal ions.

J Bérces 1, M Otos 1, S Szirmai 1, C Crane-Uruena 1, G J Köteles 1
PMCID: PMC1521140  PMID: 8143600

Abstract

The lymphocyte micronucleus assay was used to measure the average frequency of micronuclei in a population and thus assess genotoxic effects. Data from 174 persons give an average value of 16.4 +/- 7.3, and a slight age-dependence was observed. To detect combined environmental mutagen injuries the micronucleus assay was used to study the effects of metal compounds. Cadmium ions increased the micronucleus frequency linearly after incubation with whole blood in vitro with 10(6)-10(-3) M concentrations for 30 min. Similarly, a linear increase in micronucleus frequency was detected with 10(-3)-10(-1) M mercury ions. Concerning the biological effect of selenium, it was found that neither sodium selenite nor selenium dioxide induced increases at concentrations of 10(-7)-10(-6) M; 10(-5) M caused a slight increase; 10(-4) M, however, destroyed the cells. These results suggest that the human lymphocyte micronucleus test can be used to assess genotoxic injuries due to environmental effects in human lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almássy Z., Krepinsky A. B., Bianco A., Köteles G. J. The present state and perspectives of micronucleus assay in radiation protection. A review. Int J Rad Appl Instrum A. 1987;38(4):241–249. doi: 10.1016/0883-2889(87)90033-5. [DOI] [PubMed] [Google Scholar]
  2. Fenech M., Morley A. A. Cytokinesis-block micronucleus method in human lymphocytes: effect of in vivo ageing and low dose X-irradiation. Mutat Res. 1986 Jul;161(2):193–198. doi: 10.1016/0027-5107(86)90010-2. [DOI] [PubMed] [Google Scholar]
  3. Khalil A. M. The induction of chromosome aberrations in human purified peripheral blood lymphocytes following in vitro exposure to selenium. Mutat Res. 1989 Dec;224(4):503–506. doi: 10.1016/0165-1218(89)90076-1. [DOI] [PubMed] [Google Scholar]
  4. Kormos C., Köteles G. J. Micronuclei in X-irradiated human lymphocytes. Mutat Res. 1988 May;199(1):31–35. doi: 10.1016/0027-5107(88)90227-8. [DOI] [PubMed] [Google Scholar]
  5. Littlefield L. G., Sayer A. M., Frome E. L. Comparisons of dose-response parameters for radiation-induced acentric fragments and micronuclei observed in cytokinesis-arrested lymphocytes. Mutagenesis. 1989 Jul;4(4):265–270. doi: 10.1093/mutage/4.4.265. [DOI] [PubMed] [Google Scholar]
  6. Migliore L., Guidotti P., Favre C., Nardi M., Sessa M. R., Brunori E. Micronuclei in lymphocytes of young patients under antileukemic therapy. Mutat Res. 1991 Aug;263(4):243–248. doi: 10.1016/0165-7992(91)90008-r. [DOI] [PubMed] [Google Scholar]
  7. Migliore L., Parrini M., Sbrana I., Biagini C., Battaglia A., Loprieno N. Micronucleated lymphocytes in people occupationally exposed to potential environmental contaminants: the age effect. Mutat Res. 1991 Jan;256(1):13–20. doi: 10.1016/0921-8734(91)90028-a. [DOI] [PubMed] [Google Scholar]
  8. Prosser J. S., Moquet J. E., Lloyd D. C., Edwards A. A. Radiation induction of micronuclei in human lymphocytes. Mutat Res. 1988 May;199(1):37–45. doi: 10.1016/0027-5107(88)90228-x. [DOI] [PubMed] [Google Scholar]
  9. Zherbin E. A., Chukhlovin A. B., Köteles G. J., Kubasova T. A., Vashchenko V. I., Hanson K. P. Effects in vitro of cadmium ions on some membrane and nuclear parameters of normal and irradiated thymic lymphoid cells. Arch Toxicol. 1986 May;59(1):21–25. doi: 10.1007/BF00263952. [DOI] [PubMed] [Google Scholar]

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