Figure 5.

mnm loss-of-function affects eye development. (A–C) Scanning electron micrographs of female adult compound eyes containing ey:Flp-induced mosaic clones marked by white: dorsal, up; anterior, right; scale indicated in A. Genotypes: (A) wild type, (B) mnm^P, and (C) mnm^PX1. Note that the weak allele mnm^P clones are as large as wild type (arrows in A and B) but that the null allele mnm^PX1 leaves scars (arrow in C). (D–F) Sections of adult compound eyes containing eyeless:Flp-induced mosaic clones marked by white: dorsal, up; anterior, right; scale indicated in D. Genotypes: (D) wild type, (E) mnm^P, and (F) mnm^PX1. Note that the wild-type and mnm clones contain mutant (white⁻) photoreceptor cells (arrows in D–F). (G–L) Third instar eye-imaginal discs containing eyeless:Flp-induced mnm^PX1 mosaic clones (outlined), negatively marked by GFP; anterior, right and to the same scale (bar in G). Arrowheads indicate the position of the morphogenetic furrow. (G and J) GFP in white; (H and K) Elav antigen in white; (I and L) GFP in green and Elav in red. Note that mnm^PX1 clones are small, but express the differentiation marker Elav (arrow in G–I). (J–L) mnm rescuing transgene (see text). Note that the size of the mnm^PX1 clones is entirely rescued (arrows).