The legend for Figure 4 in the article “EpsinR: an ENTH Domain-containing Protein that Interacts with AP-1” by J. Hirst, A. Motley, K. Harasaki, S.Y. Peak Chew, and M.S. Robinson (Mol. Biol. Cell [2003], 14, 625–641, 10.1091/mbc.E02-09-0552) was published with an error. The corrected legend is published here:
Figure 4. Localization of epsinR. a, Western blot of homogenized A431 cells, either untreated (total) or centrifuged at high speed (sup and pellet), probed with affinity-purified anti-epsinR. b and c, COS cells were double-labeled for epsinR (b) and the AP-1 γ subunit (c). The two patterns show very good colocalization. d–e, COS cells transiently transfected with myc-tagged p56 were treated with nocodazole for 2 h, then double labeled either for epsinR (d, red in f) and tagged p56 (e, green in f) or for epsinR (g, red in i) and γ-adaptin (h, green in i). To quantify the extent of overlap, six cells double labeled for epsinR and tagged p56 and six cells double labeled for epsinR and γ-adaptin were analyzed, and individual spots were scored. Out of 1,607 spots analyzed from the cells double labeled for epsinR and γ-adaptin, 9% were positive for epsinR only, 7% were positive for γ-adaptin only, and 84% were positive for both proteins. Out of 1,192 spots analyzed from the cells double labeled for epsinR and p56, 48% were positive for epsinR only, 16% were positive for p56 only, and 36% were positive for both proteins. Scale bar, 20 μm.