Abstract
Pesticide contamination of drinking water supplies has increased over the past decade. A major concern is how exposure to combinations of low levels of pesticides, especially herbicides, could affect public health. Flow cytometric analysis was performed to determine the clastogenic potential of herbicide interaction on Chinese hamster ovary (CHO) cells. The cells were exposed to atrazine, simazine, cyanazine, and all possible combinations of these chemicals for 48 hr. Two concentrations were used for each sample: the U.S. EPA maximum contamination level (MCL) and the highest contamination level found in Illinois water supplies. Nuclei were isolated from the cells and analyzed by flow cytometry. The effects of clastogenicity were measured by the coefficient of variation (CV) of the G1 peak of whole cells and the change in CV of the largest chromosome in the flow karyotype. At both levels tested, atrazine caused chromosomal damage to the CHO cells. Simazine was observed to induce whole-cell clastogenicity but not flow karyotype damage. Cyanazine did not induce any measurable chromosomal damage in either analysis. Each of the herbicides, although all three were triazines, had different effects with respect to chromosome damage as measured by flow cytometry. CHO cells treated with a combination of atrazine and simazine, or atrazine and cyanazine, were observed to have whole-cell and flow karyotype damage. This damage was, however, equal to or less severe than the damage caused by either atrazine or simazine alone. No synergy was observed. When all three herbicides were combined, three of the four possible combinations gave no observable clastogenic response.
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- Bickham J. W., Sawin V. L., Burton D. W., McBee K. Flow-cytometric analysis of the effects of triethylenemelamine on somatic and testicular tissues of the rat. Cytometry. 1992;13(4):368–373. doi: 10.1002/cyto.990130406. [DOI] [PubMed] [Google Scholar]
- Bickham J. W., Sawin V. L., McBee K., Smolen M. J., Derr J. N. Further flow cytometric studies of the effects of triethylenemelamine on somatic and testicular tissues of the rat. Cytometry. 1994 Mar 1;15(3):222–229. doi: 10.1002/cyto.990150307. [DOI] [PubMed] [Google Scholar]
- Biradar D. P., Rayburn A. L. Flow cytogenetic analysis of whole cell clastogenicity of herbicides found in groundwater. Arch Environ Contam Toxicol. 1995 Jan;28(1):13–17. doi: 10.1007/BF00213963. [DOI] [PubMed] [Google Scholar]
- Custer T. W., Bickham J. W., Lyne T. B., Lewis T., Ruedas L. A., Custer C. M., Melancon M. J. Flow cytometry for monitoring contaminant exposure in black-crowned night-herons. Arch Environ Contam Toxicol. 1994 Aug;27(2):176–179. doi: 10.1007/BF00214260. [DOI] [PubMed] [Google Scholar]
- Lamb T., Bickham J. W., Gibbons J. W., Smolen M. J., McDowell S. Genetic damage in a population of slider turtles (Trachemys scripta) inhabiting a radioactive reservoir. Arch Environ Contam Toxicol. 1991 Jan;20(1):138–142. doi: 10.1007/BF01065340. [DOI] [PubMed] [Google Scholar]
- McBee K., Bickham J. W. Petrochemical-related DNA damage in wild rodents detected by flow cytometry. Bull Environ Contam Toxicol. 1988 Mar;40(3):343–349. doi: 10.1007/BF01689090. [DOI] [PubMed] [Google Scholar]
- Meisner L. F., Belluck D. A., Roloff B. D. Cytogenetic effects of alachlor and/or atrazine in vivo and in vitro. Environ Mol Mutagen. 1992;19(1):77–82. doi: 10.1002/em.2850190110. [DOI] [PubMed] [Google Scholar]
- Munger R., Isacson P., Hu S., Burns T., Hanson J., Lynch C. F., Cherryholmes K., Van Dorpe P., Hausler W. J., Jr Intrauterine growth retardation in Iowa communities with herbicide-contaminated drinking water supplies. Environ Health Perspect. 1997 Mar;105(3):308–314. doi: 10.1289/ehp.97105308. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Otto F. J., Oldiges H. Flow cytogenetic studies in chromosomes and whole cells for the detection of clastogenic effects. Cytometry. 1980 Jul;1(1):13–17. doi: 10.1002/cyto.990010104. [DOI] [PubMed] [Google Scholar]
- Steen H. B., Lindmo T. Flow cytometry: a high-resolution instrument for everyone. Science. 1979 Apr 27;204(4391):403–404. doi: 10.1126/science.441727. [DOI] [PubMed] [Google Scholar]
- Yoder J., Watson M., Benson W. W. Lymphocyte chromosome analysis of agricultural workers during extensive occupational exposure to pesticides. Mutat Res. 1973 Dec;21(6):335–340. [PubMed] [Google Scholar]