Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1995 Apr;100(1):173–179. doi: 10.1111/j.1365-2249.1995.tb03620.x

Human neutrophil Fc receptor-mediated adhesion under flow: a hollow fibre model of intravascular arrest.

C D'Arrigo 1, J J Candal-Couto 1, M Greer 1, D J Veale 1, J M Woof 1
PMCID: PMC1534271  PMID: 7535210

Abstract

Human polymorphonuclear cells (PMN) were found to adhere to a novel model of blood vessel wall-associated IgG. The internal surfaces of cellulose acetate hollow fibres, of comparable internal diameter to small blood vessels, were coated with normal serum human IgG, heat-aggregated IgG (HAIgG), laminin or fibrinogen. Under conditions of flow mimicking those in a small vessel, PMN were found to adhere markedly only to immunoglobulin-coated fibres. Arrest on HAIgG was inhibited by excess soluble IgG but not by bovine serum albumin (BSA), demonstrating that the adhesion was IgG-specific and presumably mediated by Fc gamma R on the PMN surface. Pre-adsorption of serum components onto HAIgG-coated fibres enhanced PMN arrest, due most probably to fixation of complement components by immobilized HAIgG, resulting in additional potential to entrap PMN via complement receptors such as CR3. Treatment of PMN with the regulatory neuropeptide substance P also enhanced adhesion to HAIgG-coated fibres and caused increased surface expression of Fc gamma RI, Fc gamma RII and Fc gamma RIII. A mouse cell line derived from L cells, hR4C6, stably transfected with human Fc gamma RII, was found to adhere under flow to HAIgG-coated fibres, whilst untransfected parent L cells did not. This adhesion was similarly inhibited by excess soluble IgG, confirming the capability of Fc gamma R to mediate cell arrest. The study strongly suggests that Fc gamma R may play an important role in intravascular PMN arrest and we speculate that in inflammatory diseases PMN may adhere via Fc gamma R to immobilized immunoglobulin on the vascular endothelium, with subsequent degranulation and tissue damage.

Full text

PDF
173

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atherton A., Born G. V. Relationship between the velocity of rolling granulocytes and that of the blood flow in venules. J Physiol. 1973 Aug;233(1):157–165. doi: 10.1113/jphysiol.1973.sp010303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Atkinson J. P. Complement deficiency: predisposing factor to autoimmune syndromes. Clin Exp Rheumatol. 1989 Sep-Oct;7 (Suppl 3):S95–101. [PubMed] [Google Scholar]
  3. Brown K. A., McCarthy D., Bull M., Chasty R. C., Dumonde D. C. Heat-induced aggregated human IgG modifies the adherence of human polymorphonuclear cells to cultured endothelium. Clin Exp Immunol. 1989 Sep;77(3):356–360. [PMC free article] [PubMed] [Google Scholar]
  4. Burton D. R., Woof J. M. Human antibody effector function. Adv Immunol. 1992;51:1–84. doi: 10.1016/s0065-2776(08)60486-1. [DOI] [PubMed] [Google Scholar]
  5. Cines D. B., Lyss A. P., Bina M., Corkey R., Kefalides N. A., Friedman H. M. Fc and C3 receptors induced by herpes simplex virus on cultured human endothelial cells. J Clin Invest. 1982 Jan;69(1):123–128. doi: 10.1172/JCI110422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Crockett-Torabi E., Fantone J. C. Soluble and insoluble immune complexes activate human neutrophil NADPH oxidase by distinct Fc gamma receptor-specific mechanisms. J Immunol. 1990 Nov 1;145(9):3026–3032. [PubMed] [Google Scholar]
  7. Davies K. A., Peters A. M., Beynon H. L., Walport M. J. Immune complex processing in patients with systemic lupus erythematosus. In vivo imaging and clearance studies. J Clin Invest. 1992 Nov;90(5):2075–2083. doi: 10.1172/JCI116090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fanger M. W., Shen L., Graziano R. F., Guyre P. M. Cytotoxicity mediated by human Fc receptors for IgG. Immunol Today. 1989 Mar;10(3):92–99. doi: 10.1016/0167-5699(89)90234-X. [DOI] [PubMed] [Google Scholar]
  9. Hashimoto Y., Hurd E. R. Human neutrophil aggregation and increased adherence to human endothelial cells induced by heat-aggregated IgG and immune complexes. Clin Exp Immunol. 1981 Jun;44(3):538–547. [PMC free article] [PubMed] [Google Scholar]
  10. Hay F. C., Nineham L. J., Perumal R., Roitt I. M. Intra-articular and circulating immune complexes and antiglobulins (IgG and IgM) in rheumatoid arthritis; correlation with clinical features. Ann Rheum Dis. 1979 Feb;38(1):1–7. doi: 10.1136/ard.38.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hibbs M. L., Bonadonna L., Scott B. M., McKenzie I. F., Hogarth P. M. Molecular cloning of a human immunoglobulin G Fc receptor. Proc Natl Acad Sci U S A. 1988 Apr;85(7):2240–2244. doi: 10.1073/pnas.85.7.2240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kimberly R. P., Ahlstrom J. W., Click M. E., Edberg J. C. The glycosyl phosphatidylinositol-linked Fc gamma RIIIPMN mediates transmembrane signaling events distinct from Fc gamma RII. J Exp Med. 1990 Apr 1;171(4):1239–1255. doi: 10.1084/jem.171.4.1239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Leeuwenberg J. F., Jeunhomme G. M., Buurman W. A. Adhesion of polymorphonuclear cells to human endothelial cells. Adhesion-molecule-dependent, and Fc receptor-mediated adhesion-molecule-independent mechanisms. Clin Exp Immunol. 1990 Sep;81(3):496–500. doi: 10.1111/j.1365-2249.1990.tb05362.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lloyds D., Hallett M. B. Activation and priming of the human neutrophil oxidase response by substance P: distinct signal transduction pathways. Biochim Biophys Acta. 1993 Jan 17;1175(2):207–213. doi: 10.1016/0167-4889(93)90024-j. [DOI] [PubMed] [Google Scholar]
  15. Marshall K. W., Chiu B., Inman R. D. Substance P and arthritis: analysis of plasma and synovial fluid levels. Arthritis Rheum. 1990 Jan;33(1):87–90. doi: 10.1002/art.1780330111. [DOI] [PubMed] [Google Scholar]
  16. Ryan U. S., Schultz D. R., Ruan J. W. Fc and C3b receptors on pulmonary endothelial cells: induction by injury. Science. 1981 Oct 30;214(4520):557–558. doi: 10.1126/science.6270789. [DOI] [PubMed] [Google Scholar]
  17. Schifferli J. A., Ng Y. C., Paccaud J. P., Walport M. J. The role of hypocomplementaemia and low erythrocyte complement receptor type 1 numbers in determining abnormal immune complex clearance in humans. Clin Exp Immunol. 1989 Mar;75(3):329–335. [PMC free article] [PubMed] [Google Scholar]
  18. Smiley J. D., Moore S. E., Jr Immune-complex vasculitis: role of complement and IgG-Fc receptor functions. Am J Med Sci. 1989 Oct;298(4):267–277. doi: 10.1097/00000441-198910000-00012. [DOI] [PubMed] [Google Scholar]
  19. Smith C. W., Marlin S. D., Rothlein R., Toman C., Anderson D. C. Cooperative interactions of LFA-1 and Mac-1 with intercellular adhesion molecule-1 in facilitating adherence and transendothelial migration of human neutrophils in vitro. J Clin Invest. 1989 Jun;83(6):2008–2017. doi: 10.1172/JCI114111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. THOMPSON M., BYWATERS E. G. Unilateral rheumatoid arthritis following hemiplegia. Ann Rheum Dis. 1962 Dec;21:370–377. doi: 10.1136/ard.21.4.370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Veale D., Farrell M., Fitzgerald O. Mechanism of joint sparing in a patient with unilateral psoriatic arthritis and a longstanding hemiplegia. Br J Rheumatol. 1993 May;32(5):413–416. doi: 10.1093/rheumatology/32.5.413. [DOI] [PubMed] [Google Scholar]
  22. Walsh D. A., Wharton J., Blake D. R., Polak J. M. Neural and endothelial regulatory peptides, their possible involvement in inflammation. Int J Tissue React. 1992;14(3):101–111. [PubMed] [Google Scholar]
  23. Zhou M., Todd R. F., 3rd, van de Winkel J. G., Petty H. R. Cocapping of the leukoadhesin molecules complement receptor type 3 and lymphocyte function-associated antigen-1 with Fc gamma receptor III on human neutrophils. Possible role of lectin-like interactions. J Immunol. 1993 Apr 1;150(7):3030–3041. [PubMed] [Google Scholar]
  24. van de Winkel J. G., Anderson C. L. Biology of human immunoglobulin G Fc receptors. J Leukoc Biol. 1991 May;49(5):511–524. doi: 10.1002/jlb.49.5.511. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES