Abstract
T lymphocytes and eosinophils are probably involved in the pathogenesis of allergic bronchopulmonary aspergillosis (ABPA), a disease characterized by pulmonary eosinophilia and high serum and lavage IgE levels. We recently developed a murine model of ABPA. To investigate the mechanisms of T lymphocyte and eosinophil recruitment to the lung in this disease, we examined the expression of ICAM-1 in the lung tissue of mouse challenged with Aspergillus fumigatus (Af) antigen. C57B1/6 mice were intranasally exposed to Af (Af group) or saline (control group) three times a week for 1, 2 or 3 weeks. On days 4, 7, 14 and 21, mice were killed and lung tissue was fixed in acetone and embedded in glycol methacrylate. Serial 2-microns sections were stained with chromotrope 2R and MoAbs against ICAM-1, CD11a/CD18 (LFA-1) and CD3. Af-challenged mice presented significant increases in eosinophil, T lymphocyte and LFA-1-positive cell count and up-regulated expression of ICAM-1 in the lung tissue at all the time points examined. ICAM-1 expression intensity correlated with the number of T lymphocytes (r = 0.59, P < 0.01), LFA-1-positive cells (r = 0.68, P < 0.001), but not of eosinophils (r = -0.24, P > 0.05). These findings suggest that up-regulation of ICAM-1 expression is involved in the inflammatory process of this murine model of ABPA, and that this up-regulation may be more relevant to the the T lymphocyte accumulation in the lung.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Altman L. C., Ayars G. H., Baker C., Luchtel D. L. Cytokines and eosinophil-derived cationic proteins upregulate intercellular adhesion molecule-1 on human nasal epithelial cells. J Allergy Clin Immunol. 1993 Oct;92(4):527–536. doi: 10.1016/0091-6749(93)90077-s. [DOI] [PubMed] [Google Scholar]
- Bentley A. M., Meng Q., Robinson D. S., Hamid Q., Kay A. B., Durham S. R. Increases in activated T lymphocytes, eosinophils, and cytokine mRNA expression for interleukin-5 and granulocyte/macrophage colony-stimulating factor in bronchial biopsies after allergen inhalation challenge in atopic asthmatics. Am J Respir Cell Mol Biol. 1993 Jan;8(1):35–42. doi: 10.1165/ajrcmb/8.1.35. [DOI] [PubMed] [Google Scholar]
- Bochner B. S., Luscinskas F. W., Gimbrone M. A., Jr, Newman W., Sterbinsky S. A., Derse-Anthony C. P., Klunk D., Schleimer R. P. Adhesion of human basophils, eosinophils, and neutrophils to interleukin 1-activated human vascular endothelial cells: contributions of endothelial cell adhesion molecules. J Exp Med. 1991 Jun 1;173(6):1553–1557. doi: 10.1084/jem.173.6.1553. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dalhoff K., Bohnet S., Braun J., Kreft B., Wiessmann K. J. Intercellular adhesion molecule 1 (ICAM-1) in the pathogenesis of mononuclear cell alveolitis in pulmonary sarcoidosis. Thorax. 1993 Nov;48(11):1140–1144. doi: 10.1136/thx.48.11.1140. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DeKruyff R. H., Turner T., Abrams J. S., Palladino M. A., Jr, Umetsu D. T. Induction of human IgE synthesis by CD4+ T cell clones. Requirement for interleukin 4 and low molecular weight B cell growth factor. J Exp Med. 1989 Nov 1;170(5):1477–1493. doi: 10.1084/jem.170.5.1477. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dustin M. L., Singer K. H., Tuck D. T., Springer T. A. Adhesion of T lymphoblasts to epidermal keratinocytes is regulated by interferon gamma and is mediated by intercellular adhesion molecule 1 (ICAM-1). J Exp Med. 1988 Apr 1;167(4):1323–1340. doi: 10.1084/jem.167.4.1323. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ensari A., Ager A., Marsh M. N., Morgan S., Moriarty K. J. Time-course of adhesion molecule expression in rectal mucosa of gluten-sensitive subjects after gluten challenge. Clin Exp Immunol. 1993 May;92(2):303–307. doi: 10.1111/j.1365-2249.1993.tb03396.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gascan H., Gauchat J. F., Roncarolo M. G., Yssel H., Spits H., de Vries J. E. Human B cell clones can be induced to proliferate and to switch to IgE and IgG4 synthesis by interleukin 4 and a signal provided by activated CD4+ T cell clones. J Exp Med. 1991 Mar 1;173(3):747–750. doi: 10.1084/jem.173.3.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gulbenkian A. R., Egan R. W., Fernandez X., Jones H., Kreutner W., Kung T., Payvandi F., Sullivan L., Zurcher J. A., Watnick A. S. Interleukin-5 modulates eosinophil accumulation in allergic guinea pig lung. Am Rev Respir Dis. 1992 Jul;146(1):263–266. doi: 10.1164/ajrccm/146.1.263. [DOI] [PubMed] [Google Scholar]
- Hamacher J., Schaberg T. Adhesion molecules in lung diseases. Lung. 1994;172(4):189–213. doi: 10.1007/BF00164437. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hansel T. T., Walker C. The migration of eosinophils into the sputum of asthmatics: the role of adhesion molecules. Clin Exp Allergy. 1992 Mar;22(3):345–356. doi: 10.1111/j.1365-2222.1992.tb03096.x. [DOI] [PubMed] [Google Scholar]
- Kurup V. P., Mauze S., Choi H., Seymour B. W., Coffman R. L. A murine model of allergic bronchopulmonary aspergillosis with elevated eosinophils and IgE. J Immunol. 1992 Jun 15;148(12):3783–3788. [PubMed] [Google Scholar]
- Melis M., Gjomarkaj M., Pace E., Malizia G., Spatafora M. Increased expression of leukocyte function associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) by alveolar macrophages of patients with pulmonary sarcoidosis. Chest. 1991 Oct;100(4):910–916. doi: 10.1378/chest.100.4.910. [DOI] [PubMed] [Google Scholar]
- Montefort S., Feather I. H., Wilson S. J., Haskard D. O., Lee T. H., Holgate S. T., Howarth P. H. The expression of leukocyte-endothelial adhesion molecules is increased in perennial allergic rhinitis. Am J Respir Cell Mol Biol. 1992 Oct;7(4):393–398. doi: 10.1165/ajrcmb/7.4.393. [DOI] [PubMed] [Google Scholar]
- Montefort S., Holgate S. T., Howarth P. H. Leucocyte-endothelial adhesion molecules and their role in bronchial asthma and allergic rhinitis. Eur Respir J. 1993 Jul;6(7):1044–1054. [PubMed] [Google Scholar]
- Montefort S., Roche W. R., Howarth P. H., Djukanovic R., Gratziou C., Carroll M., Smith L., Britten K. M., Haskard D., Lee T. H. Intercellular adhesion molecule-1 (ICAM-1) and endothelial leucocyte adhesion molecule-1 (ELAM-1) expression in the bronchial mucosa of normal and asthmatic subjects. Eur Respir J. 1992 Jul;5(7):815–823. [PubMed] [Google Scholar]
- Murali P. S., Kumar A., Choi H., Banasal N. K., Fink J. N., Kurup V. P. Aspergillus fumigatus antigen induced eosinophilia in mice is abrogated by anti-IL-5 antibody. J Leukoc Biol. 1993 Mar;53(3):264–267. doi: 10.1002/jlb.53.3.264. [DOI] [PubMed] [Google Scholar]
- Resnick M. B., Weller P. F. Mechanisms of eosinophil recruitment. Am J Respir Cell Mol Biol. 1993 Apr;8(4):349–355. doi: 10.1165/ajrcmb/8.4.349. [DOI] [PubMed] [Google Scholar]
- Venge P., Håkansson L. Current understanding of the role of the eosinophil granulocyte in asthma. Clin Exp Allergy. 1991 May;21 (Suppl 3):31–37. doi: 10.1111/j.1365-2222.1991.tb01761.x. [DOI] [PubMed] [Google Scholar]
- Wang J. M., Denis M., Fournier M., Laviolette M. Cyclosporin A increases the pulmonary eosinophilia induced by inhaled Aspergillus antigen in mice. Clin Exp Immunol. 1993 Sep;93(3):323–330. doi: 10.1111/j.1365-2249.1993.tb08180.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wegner C. D., Gundel R. H., Reilly P., Haynes N., Letts L. G., Rothlein R. Intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of asthma. Science. 1990 Jan 26;247(4941):456–459. doi: 10.1126/science.1967851. [DOI] [PubMed] [Google Scholar]
- Wegner C. D., Gundel R. H., Rothlein R., Letts L. G. Expression and probable roles of cell adhesion molecules in lung inflammation. Chest. 1992 Mar;101(3 Suppl):34S–39S. doi: 10.1378/chest.101.3_supplement.34s. [DOI] [PubMed] [Google Scholar]
- Wein M., Bochner B. S. Adhesion molecule antagonists: future therapies for allergic diseases? Eur Respir J. 1993 Oct;6(9):1239–1242. [PubMed] [Google Scholar]