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. 1993 Oct;94(1):75–79. doi: 10.1111/j.1365-2249.1993.tb05980.x

Experimental murine paracoccidioidomycosis: relationship among the dissemination of the infection, humoral and cellular immune responses.

L M Singer-Vermes 1, C B Caldeira 1, E Burger 1, L G Calich 1
PMCID: PMC1534363  PMID: 8403521

Abstract

The dissemination of Paracoccidioides brasiliensis cells to the heart, omentum/pancreas, spleen, liver and lungs, assessed by colony forming unit (CFU) counts, the levels of specific antibodies to this fungal agent (by ELISA), and the specific DTH reaction were studied in susceptible (B10.A) and resistant (A/Sn) mice. The animals were infected intraperitoneally with P. brasiliensis yeast cells and were evaluated 2, 4, 12 and 16 weeks later. The most remarkable differences between the two mouse strains were observed 16 weeks after infection, when B10.A mice displayed high numbers of CFU in all examined organs, except the heart, high antibody titres, and depressed DTH response. At this point, A/Sn mice presented low or absent CFU in all organs, low antibody titres and expressive DTH response. The CFU counts were shown to be a reliable parameter to discriminate susceptible from resistant animals. The fungal load in the most affected organs correlated with the antibody titres and was inversely correlated with the intensity of the DTH reaction. The patterns of immune response in this model mimic human paracoccidioidomycosis, in which high specific antibody levels and depressed DTH reactions are found in multifocal and severe forms of the disease.

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Selected References

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  1. Aebischer T., Moody S. F., Handman E. Persistence of virulent Leishmania major in murine cutaneous leishmaniasis: a possible hazard for the host. Infect Immun. 1993 Jan;61(1):220–226. doi: 10.1128/iai.61.1.220-226.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Angela Restrepo M. Immune response to Paracoccidioides brasiliensis in human and animal hosts. Curr Top Med Mycol. 1988;2:239–277. doi: 10.1007/978-1-4612-3730-3_7. [DOI] [PubMed] [Google Scholar]
  3. Berliner M. D., Reca M. E. Vital staining of Histoplasma capsulatum with Janus Green B. Sabouraudia. 1966 Jun;5(1):26–29. [PubMed] [Google Scholar]
  4. Brown I. N., Glynn A. A. The Ity/Lsh/Bcg gene significantly affects mouse resistance to Mycobacterium lepraemurium. Immunology. 1987 Dec;62(4):587–591. [PMC free article] [PubMed] [Google Scholar]
  5. Brummer E., Restrepo A., Stevens D. A., Azzi R., Gomez A. M., Hoyos G. L., McEwen J. G., Cano L. E., de Bedout C. Murine model of paracoccidioidomycosis. Production of fatal acute pulmonary or chronic pulmonary and disseminated disease: immunological and pathological observations. J Exp Pathol. 1984 Summer;1(3):241–255. [PubMed] [Google Scholar]
  6. Calich V. L., Burger E., Kashino S. S., Fazioli R. A., Singer-Vermes L. M. Resistance to Paracoccidioides brasiliensis in mice is controlled by a single dominant autosomal gene. Infect Immun. 1987 Aug;55(8):1919–1923. doi: 10.1128/iai.55.8.1919-1923.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Calich V. L., Singer-Vermes L. M., Siqueira A. M., Burger E. Susceptibility and resistance of inbred mice to Paracoccidioides brasiliensis. Br J Exp Pathol. 1985 Oct;66(5):585–594. [PMC free article] [PubMed] [Google Scholar]
  8. Castaneda E., Brummer E., Pappagianis D., Stevens D. A. Chronic pulmonary and disseminated paracoccidioidomycosis in mice: quantitation of progression and chronicity. J Med Vet Mycol. 1987 Dec;25(6):377–387. doi: 10.1080/02681218780000461. [DOI] [PubMed] [Google Scholar]
  9. Castaneda E., Brummer E., Perlman A. M., McEwen J. G., Stevens D. A. A culture medium for Paracoccidioides brasiliensis with high plating efficiency, and the effect of siderophores. J Med Vet Mycol. 1988;26(6):351–358. [PubMed] [Google Scholar]
  10. Defaveri J., Rezkallah-Iwasso M. T., de Franco M. F. Experimental pulmonary paracoccidioidomycosis in mice: morphology and correlation of lesions with humoral and cellular immune response. Mycopathologia. 1982 Jan 15;77(1):3–11. doi: 10.1007/BF00588649. [DOI] [PubMed] [Google Scholar]
  11. Franco M. Host-parasite relationships in paracoccidioidomycosis. J Med Vet Mycol. 1987 Feb;25(1):5–18. doi: 10.1080/02681218780000021. [DOI] [PubMed] [Google Scholar]
  12. Heinzel F. P., Sadick M. D., Holaday B. J., Coffman R. L., Locksley R. M. Reciprocal expression of interferon gamma or interleukin 4 during the resolution or progression of murine leishmaniasis. Evidence for expansion of distinct helper T cell subsets. J Exp Med. 1989 Jan 1;169(1):59–72. doi: 10.1084/jem.169.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hormaeche C. E. Natural resistance to Salmonella typhimurium in different inbred mouse strains. Immunology. 1979 Jun;37(2):311–318. [PMC free article] [PubMed] [Google Scholar]
  14. Jimenez-Finkel B. E., Murphy J. W. Induction of antigen-specific T suppressor cells by soluble Paracoccidioides brasiliensis antigen. Infect Immun. 1988 Apr;56(4):734–743. doi: 10.1128/iai.56.4.734-743.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kirkland T. N., Fierer J. Inbred mouse strains differ in resistance to lethal Coccidioides immitis infection. Infect Immun. 1983 Jun;40(3):912–916. doi: 10.1128/iai.40.3.912-916.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Makino M., Davidson W. F., Fredrickson T. N., Hartley J. W., Morse H. C., 3rd Effects of non-MHC loci on resistance to retrovirus-induced immunodeficiency in mice. Immunogenetics. 1991;33(5-6):345–351. doi: 10.1007/BF00216693. [DOI] [PubMed] [Google Scholar]
  17. Marquis G., Montplaisir S., Pelletier M., Mousseau S., Auger P. Genetic resistance to murine cryptococcosis: the beige mutation (Chédiak-Higashi syndrome) in mice. Infect Immun. 1985 Jan;47(1):288–293. doi: 10.1128/iai.47.1.288-293.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McEwen J. G., Bedoya V., Patiño M. M., Salazar M. E., Restrepo A. Experimental murine paracoccidiodomycosis induced by the inhalation of conidia. J Med Vet Mycol. 1987 Jun;25(3):165–175. doi: 10.1080/02681218780000231. [DOI] [PubMed] [Google Scholar]
  19. Mendes-Giannini M. J., Camargo M. E., Lacaz C. S., Ferreira A. W. Immunoenzymatic absorption test for serodiagnosis of paracoccidioidomycosis. J Clin Microbiol. 1984 Jul;20(1):103–108. doi: 10.1128/jcm.20.1.103-108.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mok P. W., Greer D. L. Cell-mediated immune responses in patients with paracoccidioidomycosis. Clin Exp Immunol. 1977 Apr;28(1):89–98. [PMC free article] [PubMed] [Google Scholar]
  21. Mota N. G., Rezkallah-Iwasso M. T., Peraçoli M. T., Audi R. C., Mendes R. P., Marcondes J., Marques S. A., Dillon N. L., Franco M. F. Correlation between cell-mediated immunity and clinical forms of paracoccidioidomycosis. Trans R Soc Trop Med Hyg. 1985;79(6):765–772. doi: 10.1016/0035-9203(85)90112-9. [DOI] [PubMed] [Google Scholar]
  22. Netto C. F., Vegas V. S., Sciannaméa I. M., Guarnieri D. B. Antígeno polissacarídico do Paracoccidioides brasiliensis estudo do tempo de cultivo do P. brasiliensis, necessário ao preparo do antígeno. Rev Inst Med Trop Sao Paulo. 1969 May-Jun;11(3):177–181. [PubMed] [Google Scholar]
  23. Patiño M. M., Williams D., Ahrens J., Graybill J. R. Experimental histoplasmosis in the beige mouse. J Leukoc Biol. 1987 Mar;41(3):228–235. doi: 10.1002/jlb.41.3.228. [DOI] [PubMed] [Google Scholar]
  24. Plant J., Glynn A. A. Genetics of resistance to infection with Salmonella typhimurium in mice. J Infect Dis. 1976 Jan;133(1):72–78. doi: 10.1093/infdis/133.1.72. [DOI] [PubMed] [Google Scholar]
  25. Romani L., Mocci S., Bietta C., Lanfaloni L., Puccetti P., Bistoni F. Th1 and Th2 cytokine secretion patterns in murine candidiasis: association of Th1 responses with acquired resistance. Infect Immun. 1991 Dec;59(12):4647–4654. doi: 10.1128/iai.59.12.4647-4654.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Scott P. T-cell subsets and T-cell antigens in protective immunity against experimental leishmaniasis. Curr Top Microbiol Immunol. 1990;155:35–52. doi: 10.1007/978-3-642-74983-4_3. [DOI] [PubMed] [Google Scholar]
  27. Singer-Vermes L. M., Burger E., Franco M. F., Di-Bacchi M. M., Mendes-Giannini M. J., Calich V. L. Evaluation of the pathogenicity and immunogenicity of seven Paracoccidioides brasiliensis isolates in susceptible inbred mice. J Med Vet Mycol. 1989;27(2):71–82. [PubMed] [Google Scholar]
  28. Singer-Vermes L. M., Ciavaglia M. C., Kashino S. S., Burger E., Calich V. L. The source of the growth-promoting factor(s) affects the plating efficiency of Paracoccidioides brasiliensis. J Med Vet Mycol. 1992;30(3):261–264. doi: 10.1080/02681219280000331. [DOI] [PubMed] [Google Scholar]

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