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. 1994 Nov;98(2):203–209. doi: 10.1111/j.1365-2249.1994.tb06126.x

Effects of 17 beta-oestradiol on function of lymphocytes from normal donors and patients with common variable immunodeficiency (CVID).

M Evagelatou 1, A D Webster 1, J Farrant 1
PMCID: PMC1534405  PMID: 7955523

Abstract

Effects of oestradiol (E2) have been studied on the in vitro T cell-dependent differentiation of B cells from peripheral blood and spleen using normal donors and patients with the antibody deficiency disease CVID. We also studied whether it modifies T cell DNA synthesis. The effect of E2 was examined on cultures of B cells with T cells for IL-2-driven immunoglobulin secretion or of T cells for phytohaemagglutinin (PHA)-driven DNA synthesis. Interestingly, in control experiments without E2, the normal sex difference in immunoglobulin production is reversed in CVID. The data show that for normal individuals there is no major difference between male and female donors in the in vitro actions of E2 on blood B and T lymphocytes. With normal blood B cells, E2 failed to affect IgM production, but it did inhibit IgG. In normal splenic cells, E2 increased both IgM and IgG secretion in a similar way to the tonsillar cell data previously reported. E2 on normal blood T cell DNA synthesis was stimulatory. With blood cells from CVID patients an interesting contrast was seen. As with normal B cells, E2 had no effect on IgM secretion by those CVID blood B cells able to secrete IgM. However, a difference between patients and normals was that E2 did not inhibit the IL-2-driven IgG production by those CVID B cells able to secrete IgG. For T cell function, the stimulatory effect of E2 on CVID T cell DNA was as in normal T cells. However, E2 failed to restore CVID B and T cell function to normal levels. These data suggest that there may be subtle defects in the pathway of action of E2 in CVID lymphocytes.

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Selected References

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  1. Athreya B. H., Pletcher J., Zulian F., Weiner D. B., Williams W. V. Subset-specific effects of sex hormones and pituitary gonadotropins on human lymphocyte proliferation in vitro. Clin Immunol Immunopathol. 1993 Mar;66(3):201–211. doi: 10.1006/clin.1993.1026. [DOI] [PubMed] [Google Scholar]
  2. Bryant A., Calver N. C., Toubi E., Webster A. D., Farrant J. Classification of patients with common variable immunodeficiency by B cell secretion of IgM and IgG in response to anti-IgM and interleukin-2. Clin Immunol Immunopathol. 1990 Aug;56(2):239–248. doi: 10.1016/0090-1229(90)90145-g. [DOI] [PubMed] [Google Scholar]
  3. Clerici E., Bergamasco E., Ferrario E., Villa M. L. Influence of sex steroids on the antigen-specific primary antibody response in vitro. J Clin Lab Immunol. 1991 Feb;34(2):71–78. [PubMed] [Google Scholar]
  4. Cohen J. H., Danel L., Cordier G., Saez S., Revillard J. P. Sex steroid receptors in peripheral T cells: absence of androgen receptors and restriction of estrogen receptors to OKT8-positive cells. J Immunol. 1983 Dec;131(6):2767–2771. [PubMed] [Google Scholar]
  5. Evagelatou M., Farrant J. Effect of 17 beta-estradiol on immunoglobulin secretion by human tonsillar lymphocytes in vitro. J Steroid Biochem Mol Biol. 1994 Feb;48(2-3):171–177. doi: 10.1016/0960-0760(94)90142-2. [DOI] [PubMed] [Google Scholar]
  6. Farrant J., Bryant A. E., Lever A. M., Edwards A. J., Knight S. C., Webster A. D. Defective low-density cells of dendritic morphology from the blood of patients with common variable hypogammaglobulinaemia: low immunoglobulin production on stimulation of normal B cells. Clin Exp Immunol. 1985 Jul;61(1):189–194. [PMC free article] [PubMed] [Google Scholar]
  7. Farrant J., Clark J. C., Lee H., Knight S. C., O'Brien J. Conditions for measuring DNA synthesis in PHA stimulated human lymphocytes in 20 microliters hanging drops with various cell concentrations and periods of culture. J Immunol Methods. 1980;33(4):301–312. doi: 10.1016/0022-1759(80)90001-0. [DOI] [PubMed] [Google Scholar]
  8. Farrant J. Germ-line transcripts and class switching. Clin Exp Immunol. 1994 Jan;95(1):1–2. doi: 10.1111/j.1365-2249.1994.tb06005.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Farrant J. T and B cell defects in common variable immunodeficiency. Immunol Invest. 1991 Apr;20(2):143–150. doi: 10.3109/08820139109050782. [DOI] [PubMed] [Google Scholar]
  10. Grossman C. J. Interactions between the gonadal steroids and the immune system. Science. 1985 Jan 18;227(4684):257–261. doi: 10.1126/science.3871252. [DOI] [PubMed] [Google Scholar]
  11. Hermaszewski R. A., Webster A. D. Primary hypogammaglobulinaemia: a survey of clinical manifestations and complications. Q J Med. 1993 Jan;86(1):31–42. [PubMed] [Google Scholar]
  12. Herrera L. A., Montero R., León-Cázares J. M., Rojas E., Gonsebatt M. E., Ostrosky-Wegman P. Effects of progesterone and estradiol on the proliferation of phytohemagglutinin-stimulated human lymphocytes. Mutat Res. 1992 Nov 16;270(2):211–218. doi: 10.1016/0027-5107(92)90132-l. [DOI] [PubMed] [Google Scholar]
  13. Ho P. C., Tang G. W., Lawton J. W. Lymphocyte subsets in patients with oestrogen deficiency. J Reprod Immunol. 1991 May;20(1):85–91. doi: 10.1016/0165-0378(91)90025-l. [DOI] [PubMed] [Google Scholar]
  14. Jaffe J. S., Eisenstein E., Sneller M. C., Strober W. T-cell abnormalities in common variable immunodeficiency. Pediatr Res. 1993 Jan;33(1 Suppl):S24–S28. doi: 10.1203/00006450-199305001-00128. [DOI] [PubMed] [Google Scholar]
  15. Kalman B., Olsson O., Link H., Kam-Hansen S. Estradiol potentiates poke-weed mitogen-induced B cell stimulation in multiple sclerosis and healthy subjects. Acta Neurol Scand. 1989 Apr;79(4):340–346. doi: 10.1111/j.1600-0404.1989.tb03795.x. [DOI] [PubMed] [Google Scholar]
  16. Mendelsohn J., Multer M. M., Bernheim J. L. Inhibition of human lymphocyte stimulation by steroid hormones: cytokinetic mechanisms. Clin Exp Immunol. 1977 Jan;27(1):127–134. [PMC free article] [PubMed] [Google Scholar]
  17. Mizoguchi Y., Ikemoto Y., Yamamoto S., Morisawa S. Studies on the effects of estrogen on the antibody response in asymptomatic HB virus carrier. Hepatogastroenterology. 1985 Jun;32(3):109–112. [PubMed] [Google Scholar]
  18. Neifeld J. P., Tormey D. C. Effects of steroid hormones on phytohemagglutinin-stimulated human peripheral blood lymphocytes. Transplantation. 1979 May;27(5):309–314. doi: 10.1097/00007890-197905000-00004. [DOI] [PubMed] [Google Scholar]
  19. North M. E., Spickett G. P., Allsop J., Webster A. D., Farrant J. Defective DNA synthesis by T cells in acquired 'common-variable' hypogammaglobulinaemia on stimulation with mitogens. Clin Exp Immunol. 1989 Apr;76(1):19–23. [PMC free article] [PubMed] [Google Scholar]
  20. Paavonen T., Andersson L. C., Adlercreutz H. Sex hormone regulation of in vitro immune response. Estradiol enhances human B cell maturation via inhibition of suppressor T cells in pokeweed mitogen-stimulated cultures. J Exp Med. 1981 Dec 1;154(6):1935–1945. doi: 10.1084/jem.154.6.1935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Paavonen T. Hormonal regulation of lymphocyte functions. Med Biol. 1987;65(5-6):229–240. [PubMed] [Google Scholar]
  22. Schuurs A. H., Verheul H. A. Effects of gender and sex steroids on the immune response. J Steroid Biochem. 1990 Feb;35(2):157–172. doi: 10.1016/0022-4731(90)90270-3. [DOI] [PubMed] [Google Scholar]
  23. Sorachi K., Kumagai S., Sugita M., Yodoi J., Imura H. Enhancing effect of 17 beta-estradiol on human NK cell activity. Immunol Lett. 1993 Apr;36(1):31–35. doi: 10.1016/0165-2478(93)90065-a. [DOI] [PubMed] [Google Scholar]
  24. Spickett G. P., Matamoros N., Farrant J. Lymphocyte surface phenotype in common variable immunodeficiency. Dis Markers. 1992 Mar-Apr;10(2):67–80. [PubMed] [Google Scholar]
  25. Spickett G. P., Webster A. D., Farrant J. Cellular abnormalities in common variable immunodeficiency. Immunodefic Rev. 1990;2(3):199–219. [PubMed] [Google Scholar]
  26. Sthoeger Z. M., Chiorazzi N., Lahita R. G. Regulation of the immune response by sex hormones. I. In vitro effects of estradiol and testosterone on pokeweed mitogen-induced human B cell differentiation. J Immunol. 1988 Jul 1;141(1):91–98. [PubMed] [Google Scholar]
  27. Stimson W. H. Oestrogen and human T lymphocytes: presence of specific receptors in the T-suppressor/cytotoxic subset. Scand J Immunol. 1988 Sep;28(3):345–350. doi: 10.1111/j.1365-3083.1988.tb01459.x. [DOI] [PubMed] [Google Scholar]
  28. Weetman A. P., McGregor A. M., Smith B. R., Hall R. Sex hormones enhance immunoglobulin synthesis by human peripheral blood lymphocytes. Immunol Lett. 1981 Dec;3(6):343–346. doi: 10.1016/0165-2478(81)90064-x. [DOI] [PubMed] [Google Scholar]
  29. Wright J. J., Wagner D. K., Blaese R. M., Hagengruber C., Waldmann T. A., Fleisher T. A. Characterization of common variable immunodeficiency: identification of a subset of patients with distinctive immunophenotypic and clinical features. Blood. 1990 Nov 15;76(10):2046–2051. [PubMed] [Google Scholar]
  30. Wyle F. A., Kent J. R. Immunosuppression by sex steroid hormones. The effect upon PHA- and PPD-stimulated lymphocytes. Clin Exp Immunol. 1977 Mar;27(3):407–415. [PMC free article] [PubMed] [Google Scholar]
  31. Yron I., Langer A., Weinstein T., Sahar E., Lidor Y., Pardo Y., Katz I., Shohat L., Kalechman Y., Ovadia J. Effect of sex hormones on human T cell activation by concanavalin A. Nat Immun Cell Growth Regul. 1991;10(1):32–44. [PubMed] [Google Scholar]
  32. van den Brink H. R., van Wijk M. J., Bijlsma J. W. Influence of steroid hormones on proliferation of peripheral blood mononuclear cells in patients with rheumatoid arthritis. Br J Rheumatol. 1992 Oct;31(10):663–667. doi: 10.1093/rheumatology/31.10.663. [DOI] [PubMed] [Google Scholar]

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