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. 1994 Nov;98(2):319–322. doi: 10.1111/j.1365-2249.1994.tb06144.x

Transforming growth factor-beta 1 (TGF-beta 1)- and beta 2-like activities in malignant pleural effusions caused by malignant mesothelioma or primary lung cancer.

J Maeda 1, N Ueki 1, T Ohkawa 1, N Iwahashi 1, T Nakano 1, T Hada 1, K Higashino 1
PMCID: PMC1534420  PMID: 7955539

Abstract

We investigated the levels of TGF-beta in malignant pleural effusions (MPE) caused by malignant mesothelioma (MESO) or primary lung cancer. TGF-beta levels in MPE caused by MESO were 283.9 +/- 219.2 pm (mean +/- s.d.) and were three to six times higher than those due to primary lung cancers (P < 0.01 or P < 0.05). We also evaluated TGF-beta 1- and beta 2-like activities in MPE using specific polyclonal antibodies. Although TGF-beta 1-like activity could be detected in all cases, TGF-beta 2-like activities were detected in five of seven in MESO and in a few cases with primary lung cancer. These results demonstrate that the levels of total TGF-beta and TGF-beta 2-like activity may be clinically useful to differentiate MESO from primary lung cancer. Our data also suggest that TGF-beta may help further characterize the clinical features of MESO.

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Selected References

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  1. Altman D. J., Schneider S. L., Thompson D. A., Cheng H. L., Tomasi T. B. A transforming growth factor beta 2 (TGF-beta 2)-like immunosuppressive factor in amniotic fluid and localization of TGF-beta 2 mRNA in the pregnant uterus. J Exp Med. 1990 Nov 1;172(5):1391–1401. doi: 10.1084/jem.172.5.1391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bergh J. The expression of the platelet-derived and transforming growth factor genes in human nonsmall lung cancer cell lines is related to tumor stroma formation in nude mice tumors. Am J Pathol. 1988 Dec;133(3):434–439. [PMC free article] [PubMed] [Google Scholar]
  3. Danielpour D., Dart L. L., Flanders K. C., Roberts A. B., Sporn M. B. Immunodetection and quantitation of the two forms of transforming growth factor-beta (TGF-beta 1 and TGF-beta 2) secreted by cells in culture. J Cell Physiol. 1989 Jan;138(1):79–86. doi: 10.1002/jcp.1041380112. [DOI] [PubMed] [Google Scholar]
  4. Dubaybo B. A., Thet L. A. Effect of transforming growth factor beta on synthesis of glycosaminoglycans by human lung fibroblasts. Exp Lung Res. 1990 Sep-Oct;16(5):389–403. doi: 10.3109/01902149009068816. [DOI] [PubMed] [Google Scholar]
  5. Gabrielson E. W., Gerwin B. I., Harris C. C., Roberts A. B., Sporn M. B., Lechner J. F. Stimulation of DNA synthesis in cultured primary human mesothelial cells by specific growth factors. FASEB J. 1988 Aug;2(11):2717–2721. doi: 10.1096/fasebj.2.11.3260881. [DOI] [PubMed] [Google Scholar]
  6. Gerwin B. I., Lechner J. F., Reddel R. R., Roberts A. B., Robbins K. C., Gabrielson E. W., Harris C. C. Comparison of production of transforming growth factor-beta and platelet-derived growth factor by normal human mesothelial cells and mesothelioma cell lines. Cancer Res. 1987 Dec 1;47(23):6180–6184. [PubMed] [Google Scholar]
  7. Heldin P., Asplund T., Ytterberg D., Thelin S., Laurent T. C. Characterization of the molecular mechanism involved in the activation of hyaluronan synthetase by platelet-derived growth factor in human mesothelial cells. Biochem J. 1992 Apr 1;283(Pt 1):165–170. doi: 10.1042/bj2830165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ikeda T., Lioubin M. N., Marquardt H. Human transforming growth factor type beta 2: production by a prostatic adenocarcinoma cell line, purification, and initial characterization. Biochemistry. 1987 May 5;26(9):2406–2410. doi: 10.1021/bi00383a002. [DOI] [PubMed] [Google Scholar]
  9. Jetten A. M., Shirley J. E., Stoner G. Regulation of proliferation and differentiation of respiratory tract epithelial cells by TGF beta. Exp Cell Res. 1986 Dec;167(2):539–549. doi: 10.1016/0014-4827(86)90193-x. [DOI] [PubMed] [Google Scholar]
  10. Kehrl J. H., Wakefield L. M., Roberts A. B., Jakowlew S., Alvarez-Mon M., Derynck R., Sporn M. B., Fauci A. S. Production of transforming growth factor beta by human T lymphocytes and its potential role in the regulation of T cell growth. J Exp Med. 1986 May 1;163(5):1037–1050. doi: 10.1084/jem.163.5.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Khalil N., O'Connor R. N., Unruh H. W., Warren P. W., Flanders K. C., Kemp A., Bereznay O. H., Greenberg A. H. Increased production and immunohistochemical localization of transforming growth factor-beta in idiopathic pulmonary fibrosis. Am J Respir Cell Mol Biol. 1991 Aug;5(2):155–162. doi: 10.1165/ajrcmb/5.2.155. [DOI] [PubMed] [Google Scholar]
  12. Lauber B., Leuthold M., Schmitter D., Cano-Santos J., Waibel R., Stahel R. A. An autocrine mitogenic activity produced by a pleural human mesothelioma cell line. Int J Cancer. 1992 Apr 1;50(6):943–950. doi: 10.1002/ijc.2910500621. [DOI] [PubMed] [Google Scholar]
  13. Leitzel K., Bryce W., Tomita J., Manderino G., Tribby I., Thomason A., Billingsley M., Podczaski E., Harvey H., Bartholomew M. Elevated plasma platelet-derived growth factor B-chain levels in cancer patients. Cancer Res. 1991 Aug 15;51(16):4149–4154. [PubMed] [Google Scholar]
  14. Maeda J., Ueki N., Ohkawa T., Iwahashi N., Nakano T., Hada T., Higashino K. Local production and localization of transforming growth factor-beta in tuberculous pleurisy. Clin Exp Immunol. 1993 Apr;92(1):32–38. doi: 10.1111/j.1365-2249.1993.tb05944.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Miyazono K., Olofsson A., Colosetti P., Heldin C. H. A role of the latent TGF-beta 1-binding protein in the assembly and secretion of TGF-beta 1. EMBO J. 1991 May;10(5):1091–1101. doi: 10.1002/j.1460-2075.1991.tb08049.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Muslin A. J., Williams L. T. Well-defined growth factors promote cardiac development in axolotl mesodermal explants. Development. 1991 Aug;112(4):1095–1101. doi: 10.1242/dev.112.4.1095. [DOI] [PubMed] [Google Scholar]
  17. Pelton R. W., Dickinson M. E., Moses H. L., Hogan B. L. In situ hybridization analysis of TGF beta 3 RNA expression during mouse development: comparative studies with TGF beta 1 and beta 2. Development. 1990 Oct;110(2):609–620. doi: 10.1242/dev.110.2.609. [DOI] [PubMed] [Google Scholar]
  18. Pelton R. W., Saxena B., Jones M., Moses H. L., Gold L. I. Immunohistochemical localization of TGF beta 1, TGF beta 2, and TGF beta 3 in the mouse embryo: expression patterns suggest multiple roles during embryonic development. J Cell Biol. 1991 Nov;115(4):1091–1105. doi: 10.1083/jcb.115.4.1091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Postlethwaite A. E., Keski-Oja J., Moses H. L., Kang A. H. Stimulation of the chemotactic migration of human fibroblasts by transforming growth factor beta. J Exp Med. 1987 Jan 1;165(1):251–256. doi: 10.1084/jem.165.1.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rosa F., Roberts A. B., Danielpour D., Dart L. L., Sporn M. B., Dawid I. B. Mesoderm induction in amphibians: the role of TGF-beta 2-like factors. Science. 1988 Feb 12;239(4841 Pt 1):783–785. doi: 10.1126/science.3422517. [DOI] [PubMed] [Google Scholar]
  21. Söderdahl G., Betsholtz C., Johansson A., Nilsson K., Bergh J. Differential expression of platelet-derived growth factor and transforming growth factor genes in small- and non-small-cell human lung carcinoma lines. Int J Cancer. 1988 Apr 15;41(4):636–641. doi: 10.1002/ijc.2910410426. [DOI] [PubMed] [Google Scholar]
  22. Wallick S. C., Figari I. S., Morris R. E., Levinson A. D., Palladino M. A. Immunoregulatory role of transforming growth factor beta (TGF-beta) in development of killer cells: comparison of active and latent TGF-beta 1. J Exp Med. 1990 Dec 1;172(6):1777–1784. doi: 10.1084/jem.172.6.1777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Welch D. R., Fabra A., Nakajima M. Transforming growth factor beta stimulates mammary adenocarcinoma cell invasion and metastatic potential. Proc Natl Acad Sci U S A. 1990 Oct;87(19):7678–7682. doi: 10.1073/pnas.87.19.7678. [DOI] [PMC free article] [PubMed] [Google Scholar]

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