Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1993 Dec;94(3):423–428.

Increased proportion of responders to a murine anti-CD3 monoclonal antibody of the IgG1 class in patients with systemic lupus erythematosus (SLE).

A M Blasini 1, I L Stekman 1, M Leon-Ponte 1, D Caldera 1, M A Rodriguez 1
PMCID: PMC1534425  PMID: 8252802

Abstract

A group of Venezuelan patients with SLE showed an increased proportion of responders to Leu-4, an anti-CD3 MoAb of the IgG1 class, compared with ethnically matched non-SLE patients and healthy controls. The rate of proliferative responses or IL-2 production induced by MoAb Leu-4, and the helper effect of macrophages from Leu-4 responders on T cells from a third-party donor were comparable in patients and controls. No significant differences in the binding of murine IgG1 molecules by macrophages from SLE patients and controls were observed. The proportion of monocytes/macrophages expressing Fc gamma RI was significantly higher in SLE patients. However, the expression of FcRII, the type capable of supporting Leu-4-mediated responses, and of Fc gamma RIII was comparable in monocytes from SLE patients and controls. Our results suggest that Venezuelan patients with SLE may have a genetic predisposition for the expression of the phenotypic variant of Fc gamma RII capable of binding murine IgG1 molecules.

Full text

PDF
423

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AboT, Tilden A. B., Balch C. M., Kumagai K., Troup G. M., Cooper M. D. Ethnic differences in the lymphocyte proliferative response induced by a murine IgG1 antibody, Leu-4, to the T3 molecule. J Exp Med. 1984 Jul 1;160(1):303–309. doi: 10.1084/jem.160.1.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson C. L., Ryan D. H., Looney R. J., Leary P. C. Structural polymorphism of the human monocyte 40 kilodalton Fc receptor for IgG. J Immunol. 1987 Apr 1;138(7):2254–2256. [PubMed] [Google Scholar]
  3. Bank I., Chess L. Perturbation of the T4 molecule transmits a negative signal to T cells. J Exp Med. 1985 Oct 1;162(4):1294–1303. doi: 10.1084/jem.162.4.1294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baroja M. L., Ceuppens J. L. More exact quantification of interleukin-2 production by addition of anti-Tac monoclonal antibody to cultures of stimulated lymphocytes. J Immunol Methods. 1987 Apr 16;98(2):267–270. doi: 10.1016/0022-1759(87)90014-7. [DOI] [PubMed] [Google Scholar]
  5. Boros P., Muryoi T., Spiera H., Bona C., Unkeless J. C. Autoantibodies directed against different classes of Fc gamma R are found in sera of autoimmune patients. J Immunol. 1993 Mar 1;150(5):2018–2024. [PubMed] [Google Scholar]
  6. Ceuppens J. L., Meurs L., Van Wauwe J. P. Failure of OKT3 monoclonal antibody to induce lymphocyte mitogenesis: a familial defect in monocyte helper function. J Immunol. 1985 Mar;134(3):1498–1502. [PubMed] [Google Scholar]
  7. Chan A. C., Irving B. A., Weiss A. New insights into T-cell antigen receptor structure and signal transduction. Curr Opin Immunol. 1992 Jun;4(3):246–251. doi: 10.1016/0952-7915(92)90072-m. [DOI] [PubMed] [Google Scholar]
  8. Clark M. R., Clarkson S. B., Ory P. A., Stollman N., Goldstein I. M. Molecular basis for a polymorphism involving Fc receptor II on human monocytes. J Immunol. 1989 Sep 1;143(5):1731–1734. [PubMed] [Google Scholar]
  9. Elkon K. B., Bonfa E., Llovet R., Eisenberg R. A. Association between anti-Sm and anti-ribosomal P protein autoantibodies in human systemic lupus erythematosus and MRL/lpr mice. J Immunol. 1989 Sep 1;143(5):1549–1554. [PubMed] [Google Scholar]
  10. Fries L. F., Mullins W. W., Cho K. R., Plotz P. H., Frank M. M. Monocyte receptors for the Fc portion of IgG are increased in systemic lupus erythematosus. J Immunol. 1984 Feb;132(2):695–700. [PubMed] [Google Scholar]
  11. Jazwinska E. C., Olive C., Hogarth P. M., Gatenby P. A., Serjeantson S. W. Fc gamma RII restriction fragment length polymorphism (RFLP): analysis in systemic lupus erythematosus and scleroderma and evidence of an alpha gene duplication. Clin Exp Immunol. 1991 Jan;83(1):47–51. doi: 10.1111/j.1365-2249.1991.tb05586.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kaneoka H., Morito F., Yamaguchi M. Low responsiveness to the anti Leu 4 antibody by T cells from patients with active systemic lupus erythematosus. J Clin Lab Immunol. 1989 Jan;28(1):15–26. [PubMed] [Google Scholar]
  13. Kaneoka H., Perez-Rojas G., Sasasuki T., Benike C. J., Engleman E. G. Human T lymphocyte proliferation induced by a pan-T monoclonal antibody (anti-Leu 4): heterogeneity of response is a function of monocytes. J Immunol. 1983 Jul;131(1):158–164. [PubMed] [Google Scholar]
  14. Parren P. W., Warmerdam P. A., Boeije L. C., Arts J., Westerdaal N. A., Vlug A., Capel P. J., Aarden L. A., van de Winkel J. G. On the interaction of IgG subclasses with the low affinity Fc gamma RIIa (CD32) on human monocytes, neutrophils, and platelets. Analysis of a functional polymorphism to human IgG2. J Clin Invest. 1992 Oct;90(4):1537–1546. doi: 10.1172/JCI116022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rankin B. M., Yocum S. A., Mittler R. S., Kiener P. A. Stimulation of tyrosine phosphorylation and calcium mobilization by Fc gamma receptor cross-linking. Regulation by the phosphotyrosine phosphatase CD45. J Immunol. 1993 Jan 15;150(2):605–616. [PubMed] [Google Scholar]
  16. Rodriguez M. A., Blanca I., Baroja M. L., Arama S., Leon-Ponte M., Abadi I., Bianco N. E. Helper activity by human large granular lymphocytes in in vitro immunoglobulin synthesis. J Clin Immunol. 1987 Sep;7(5):356–364. doi: 10.1007/BF00917013. [DOI] [PubMed] [Google Scholar]
  17. Rubin R. L., Tang F. L., Lucas A. H., Spiegelberg H. L., Tan E. M. IgG subclasses of anti-tetanus toxoid antibodies in adult and newborn normal subjects and in patients with systemic lupus erythematosus, Sjogren's syndrome, and drug-induced autoimmunity. J Immunol. 1986 Oct 15;137(8):2522–2527. [PubMed] [Google Scholar]
  18. Salmon J. E., Kimberly R. P., Gibofsky A., Fotino M. Defective mononuclear phagocyte function in systemic lupus erythematosus: dissociation of Fc receptor-ligand binding and internalization. J Immunol. 1984 Nov;133(5):2525–2531. [PubMed] [Google Scholar]
  19. Stekman I. L., Blasini A. M., Leon-Ponte M., Baroja M. L., Abadi I., Rodriguez M. A. Enhanced CD3-mediated T lymphocyte proliferation in patients with systemic lupus erythematosus. Arthritis Rheum. 1991 Apr;34(4):459–467. doi: 10.1002/art.1780340411. [DOI] [PubMed] [Google Scholar]
  20. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]
  21. Tax W. J., Hermes F. F., Willems R. W., Capel P. J., Koene R. A. Fc receptors for mouse IgG1 on human monocytes: polymorphism and role in antibody-induced T cell proliferation. J Immunol. 1984 Sep;133(3):1185–1189. [PubMed] [Google Scholar]
  22. Tax W. J., Willems H. W., Reekers P. P., Capel P. J., Koene R. A. Polymorphism in mitogenic effect of IgG1 monoclonal antibodies against T3 antigen on human T cells. Nature. 1983 Aug 4;304(5925):445–447. doi: 10.1038/304445a0. [DOI] [PubMed] [Google Scholar]
  23. Unkeless J. C., Scigliano E., Freedman V. H. Structure and function of human and murine receptors for IgG. Annu Rev Immunol. 1988;6:251–281. doi: 10.1146/annurev.iy.06.040188.001343. [DOI] [PubMed] [Google Scholar]
  24. Urowitz M. B., Gladman D. D., Tozman E. C., Goldsmith C. H. The lupus activity criteria count (LACC). J Rheumatol. 1984 Dec;11(6):783–787. [PubMed] [Google Scholar]
  25. Van Wauwe J. P., Goossens J. G. The mitogenic activity of OKT3 and anti-Leu 4 monoclonal antibodies: a comparative study. Cell Immunol. 1983 Apr 1;77(1):23–29. doi: 10.1016/0008-8749(83)90003-5. [DOI] [PubMed] [Google Scholar]
  26. Warmerdam P. A., van de Winkel J. G., Vlug A., Westerdaal N. A., Capel P. J. A single amino acid in the second Ig-like domain of the human Fc gamma receptor II is critical for human IgG2 binding. J Immunol. 1991 Aug 15;147(4):1338–1343. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES