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. 1994 Dec;98(3):526–531. doi: 10.1111/j.1365-2249.1994.tb05523.x

Flow cytometry analysis of OKT4 epitope deficiency in South African black children.

E J Hughes 1, E A Goddard 1, P Bouic 1, D W Beatty 1
PMCID: PMC1534499  PMID: 7527746

Abstract

A case of OKT4 epitope deficiency referred for investigation with suspected immunodeficiency is described. Flow cytometry analysis of OKT4 epitope deficiency in a study group of healthy black children showed different manifestations of the lack of OKT4 epitope; a complete lack of OKT4+CD4+ peripheral blood lymphocytes (PBL) with normal numbers of OKT4A+ and Leu3a-CD4+ PBL, decreased percentage OKT4+CD4+ compared with OKT4A+ and Leu-3a+CD4+ PBL, decreased fluorescent staining intensity with OKT4 and a biphasic OKT4 staining pattern associated with a reduced OKT4/Leu-3a ratio. The percentage and fluorescent intensity of OKT4+CD4+ PBL in the study group were significantly lower (P < 0.0001) than Leu-3a+CD4+ and OKT4A+CD4+ PBL. There is thus considerable risk of under-estimating the number of CD4+ cells in black South Africans if the OKT4 MoAb is used.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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