Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1994 Apr;96(1):31–35. doi: 10.1111/j.1365-2249.1994.tb06225.x

Experimental arthritis induced by continuous infusion of IL-8 into rabbit knee joints.

H Endo 1, T Akahoshi 1, A Nishimura 1, M Tonegawa 1, K Takagishi 1, S Kashiwazaki 1, K Matsushima 1, H Kondo 1
PMCID: PMC1534528  PMID: 7512008

Abstract

To determine the roles of IL-8 in inflammatory synovitis, examination was made of the results of continuously injecting human recombinant IL-8 into the knee joints of New Zealand while rabbits. Recombinant human IL-8 was infused continuously into the joint cavity at 75 ng/h for 14 days by a polypropylene catheter connected to a mini-osmotic pump implanted in each rabbit. Infiltration of inflammatory cells into joint cavity and histopathological changes in synovial tissue were examined at 7 and 14 days following the start of infusion. The continuous infusion of IL-8 for 14 days led to severe arthritis characterized by apparent erythema and joint pain, the accumulation of leucocytes, infiltration of mononuclear cells in synovial tissue, and marked hypervascularization in the synovial lining layer. IL-8 may be a factor which can contribute to the inflammatory process of chronic arthritis by mediating leucocyte recruitment and hypervascularization in inflamed joints.

Full text

PDF
31

Images in this article

33Fig. 2
on p.33

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cunha F. Q., Lorenzetti B. B., Poole S., Ferreira S. H. Interleukin-8 as a mediator of sympathetic pain. Br J Pharmacol. 1991 Nov;104(3):765–767. doi: 10.1111/j.1476-5381.1991.tb12502.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Edwards J. C., Read N., Trefty B., Coulstock J., Henderson B. Quantitative histological analysis of antigen-induced arthritis in the rabbit. Br J Exp Pathol. 1988 Oct;69(5):739–748. [PMC free article] [PubMed] [Google Scholar]
  3. Endo H., Akahoshi T., Takagishi K., Kashiwazaki S., Matsushima K. Elevation of interleukin-8 (IL-8) levels in joint fluids of patients with rheumatoid arthritis and the induction by IL-8 of leukocyte infiltration and synovitis in rabbit joints. Lymphokine Cytokine Res. 1991 Aug;10(4):245–252. [PubMed] [Google Scholar]
  4. Feige U., Karbowski A., Rordorf-Adam C., Pataki A. Arthritis induced by continuous infusion of hr-interleukin-1 alpha into the rabbit knee-joint. Int J Tissue React. 1989;11(5):225–238. [PubMed] [Google Scholar]
  5. Furuta R., Yamagishi J., Kotani H., Sakamoto F., Fukui T., Matsui Y., Sohmura Y., Yamada M., Yoshimura T., Larsen C. G. Production and characterization of recombinant human neutrophil chemotactic factor. J Biochem. 1989 Sep;106(3):436–441. doi: 10.1093/oxfordjournals.jbchem.a122870. [DOI] [PubMed] [Google Scholar]
  6. Hirota K., Akahoshi T., Endo H., Kondo H., Kashiwazaki S. Production of interleukin 8 by cultured synovial cells in response to interleukin 1 and tumor necrosis factor. Rheumatol Int. 1992;12(1):13–16. doi: 10.1007/BF00246871. [DOI] [PubMed] [Google Scholar]
  7. Koch A. E., Kunkel S. L., Burrows J. C., Evanoff H. L., Haines G. K., Pope R. M., Strieter R. M. Synovial tissue macrophage as a source of the chemotactic cytokine IL-8. J Immunol. 1991 Oct 1;147(7):2187–2195. [PubMed] [Google Scholar]
  8. Koch A. E., Polverini P. J., Kunkel S. L., Harlow L. A., DiPietro L. A., Elner V. M., Elner S. G., Strieter R. M. Interleukin-8 as a macrophage-derived mediator of angiogenesis. Science. 1992 Dec 11;258(5089):1798–1801. doi: 10.1126/science.1281554. [DOI] [PubMed] [Google Scholar]
  9. Koch A. E., Polverini P. J., Leibovich S. J. Stimulation of neovascularization by human rheumatoid synovial tissue macrophages. Arthritis Rheum. 1986 Apr;29(4):471–479. doi: 10.1002/art.1780290403. [DOI] [PubMed] [Google Scholar]
  10. Larsen C. G., Anderson A. O., Appella E., Oppenheim J. J., Matsushima K. The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes. Science. 1989 Mar 17;243(4897):1464–1466. doi: 10.1126/science.2648569. [DOI] [PubMed] [Google Scholar]
  11. Matsushima K., Oppenheim J. J. Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF. Cytokine. 1989 Nov;1(1):2–13. doi: 10.1016/1043-4666(89)91043-0. [DOI] [PubMed] [Google Scholar]
  12. Rampart M., Van Damme J., Zonnekeyn L., Herman A. G. Granulocyte chemotactic protein/interleukin-8 induces plasma leakage and neutrophil accumulation in rabbit skin. Am J Pathol. 1989 Jul;135(1):21–25. [PMC free article] [PubMed] [Google Scholar]
  13. Sano H., Forough R., Maier J. A., Case J. P., Jackson A., Engleka K., Maciag T., Wilder R. L. Detection of high levels of heparin binding growth factor-1 (acidic fibroblast growth factor) in inflammatory arthritic joints. J Cell Biol. 1990 Apr;110(4):1417–1426. doi: 10.1083/jcb.110.4.1417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. White M. V., Yoshimura T., Hook W., Kaliner M. A., Leonard E. J. Neutrophil attractant/activation protein-1 (NAP-1) causes human basophil histamine release. Immunol Lett. 1989 Aug;22(2):151–154. doi: 10.1016/0165-2478(89)90182-x. [DOI] [PubMed] [Google Scholar]
  15. Wilder R. L., Lafyatis R., Roberts A. B., Case J. P., Kumkumian G. K., Sano H., Sporn M. B., Remmers E. F. Transforming growth factor-beta in rheumatoid arthritis. Ann N Y Acad Sci. 1990;593:197–207. doi: 10.1111/j.1749-6632.1990.tb16112.x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES