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. 1994 Apr;96(1):75–78. doi: 10.1111/j.1365-2249.1994.tb06233.x

Comparison of the immune response against Mycobacterium tuberculosis antigens between a group of patients with active pulmonary tuberculosis and healthy household contacts.

M Torres 1, P Mendez-Sampeiro 1, L Jimenez-Zamudio 1, L Teran 1, A Camarena 1, R Quezada 1, E Ramos 1, E Sada 1
PMCID: PMC1534541  PMID: 8149670

Abstract

The mycobacterial antigens and the factors related to protection for the development of active tuberculosis are not known. In a natural model of tuberculosis, we studied 10 patients with active pulmonary tuberculosis (non-protective immune response) and 38 healthy household contacts (protective immune response). We tested the lymphocyte proliferative response by T cell Western blotting to eight different antigen fractions and to two purified mycobacterial antigens of 30 and 64 kD. Patients with active tuberculosis recognized fractions with molecular weights of 80-114, 60-80, 28-41 and 14-19 kD. Household contacts recognized the same fractions except the 14-19 kD. The response to the 64-kD antigen was not significantly different between groups. In contrast, 10% of the patients with active tuberculosis and 73% of the household contacts responded to the 30-kD antigen. The humoral response against the 30-kD antigen by ELISA showed a significantly higher production of antibodies in tuberculosis patients compared with household contacts. We conclude that patients with active pulmonary tuberculosis develop an immune response characterized by poor proliferative response to the 30-kD antigen with a strong humoral response, whereas the opposite occurs in healthy subjects infected by Mycobacterium tuberculosis.

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Selected References

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  1. Abou-Zeid C., Filley E., Steele J., Rook G. A. A simple new method for using antigens separated by polyacrylamide gel electrophoresis to stimulate lymphocytes in vitro after converting bands cut from Western blots into antigen-bearing particles. J Immunol Methods. 1987 Apr 2;98(1):5–10. doi: 10.1016/0022-1759(87)90429-7. [DOI] [PubMed] [Google Scholar]
  2. Abou-Zeid C., Ratliff T. L., Wiker H. G., Harboe M., Bennedsen J., Rook G. A. Characterization of fibronectin-binding antigens released by Mycobacterium tuberculosis and Mycobacterium bovis BCG. Infect Immun. 1988 Dec;56(12):3046–3051. doi: 10.1128/iai.56.12.3046-3051.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Andersen P., Heron I. Specificity of a protective memory immune response against Mycobacterium tuberculosis. Infect Immun. 1993 Mar;61(3):844–851. doi: 10.1128/iai.61.3.844-851.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barnes P. F., Mehra V., Hirschfield G. R., Fong S. J., Abou-Zeid C., Rook G. A., Hunter S. W., Brennan P. J., Modlin R. L. Characterization of T cell antigens associated with the cell wall protein-peptidoglycan complex of Mycobacterium tuberculosis. J Immunol. 1989 Oct 15;143(8):2656–2662. [PubMed] [Google Scholar]
  5. Falla J. C., Parra C. A., Mendoza M., Franco L. C., Guzmán F., Forero J., Orozco O., Patarroyo M. E. Identification of B- and T-cell epitopes within the MTP40 protein of Mycobacterium tuberculosis and their correlation with the disease course. Infect Immun. 1991 Jul;59(7):2265–2273. doi: 10.1128/iai.59.7.2265-2273.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hahn H., Kaufmann S. H. The role of cell-mediated immunity in bacterial infections. Rev Infect Dis. 1981 Nov-Dec;3(6):1221–1250. doi: 10.1093/clinids/3.6.1221. [DOI] [PubMed] [Google Scholar]
  7. Harboe M., Wiker H. G., Nagai S. Protein antigens of mycobacteria studied by quantitative immunologic techniques. Clin Infect Dis. 1992 Jan;14(1):313–319. doi: 10.1093/clinids/14.1.313. [DOI] [PubMed] [Google Scholar]
  8. Havlir D. V., Wallis R. S., Boom W. H., Daniel T. M., Chervenak K., Ellner J. J. Human immune response to Mycobacterium tuberculosis antigens. Infect Immun. 1991 Feb;59(2):665–670. doi: 10.1128/iai.59.2.665-670.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Huygen K., Van Vooren J. P., Turneer M., Bosmans R., Dierckx P., De Bruyn J. Specific lymphoproliferation, gamma interferon production, and serum immunoglobulin G directed against a purified 32 kDa mycobacterial protein antigen (P32) in patients with active tuberculosis. Scand J Immunol. 1988 Feb;27(2):187–194. doi: 10.1111/j.1365-3083.1988.tb02338.x. [DOI] [PubMed] [Google Scholar]
  10. Kochi A. The global tuberculosis situation and the new control strategy of the World Health Organization. Tubercle. 1991 Mar;72(1):1–6. doi: 10.1016/0041-3879(91)90017-m. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Lamb J. R., Bal V., Rothbard J. B., Mehlert A., Mendez-Samperio P., Young D. B. The mycobacterial GroEL stress protein: a common target of T-cell recognition in infection and autoimmunity. J Autoimmun. 1989 Jun;2 (Suppl):93–100. doi: 10.1016/0896-8411(89)90120-0. [DOI] [PubMed] [Google Scholar]
  13. Lee S. P., Stoker N. G., Grant K. A., Handzel Z. T., Hussain R., McAdam K. P., Dockrell H. M. Cellular immune responses of leprosy contacts to fractionated Mycobacterium leprae antigens. Infect Immun. 1989 Aug;57(8):2475–2480. doi: 10.1128/iai.57.8.2475-2480.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lenzini L., Rottoli P., Rottoli L. The spectrum of human tuberculosis. Clin Exp Immunol. 1977 Feb;27(2):230–237. [PMC free article] [PubMed] [Google Scholar]
  15. Mendez-Samperio P., Lamb J., Bothamley G., Stanley P., Ellis C., Ivanyi J. Molecular study of the T cell repertoire in family contacts and patients with leprosy. J Immunol. 1989 May 15;142(10):3599–3604. [PubMed] [Google Scholar]
  16. Nagai S., Wiker H. G., Harboe M., Kinomoto M. Isolation and partial characterization of major protein antigens in the culture fluid of Mycobacterium tuberculosis. Infect Immun. 1991 Jan;59(1):372–382. doi: 10.1128/iai.59.1.372-382.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sada E., Ferguson L. E., Daniel T. M. An ELISA for the serodiagnosis of tuberculosis using a 30,000-Da native antigen of Mycobacterium tuberculosis. J Infect Dis. 1990 Oct;162(4):928–931. doi: 10.1093/infdis/162.4.928. [DOI] [PubMed] [Google Scholar]
  18. Salata R. A., Sanson A. J., Malhotra I. J., Wiker H. G., Harboe M., Phillips N. B., Daniel T. M. Purification and characterization of the 30,000 dalton native antigen of Mycobacterium tuberculosis and characterization of six monoclonal antibodies reactive with a major epitope of this antigen. J Lab Clin Med. 1991 Dec;118(6):589–598. [PubMed] [Google Scholar]
  19. Yamamura M., Uyemura K., Deans R. J., Weinberg K., Rea T. H., Bloom B. R., Modlin R. L. Defining protective responses to pathogens: cytokine profiles in leprosy lesions. Science. 1991 Oct 11;254(5029):277–279. doi: 10.1126/science.254.5029.277. [DOI] [PubMed] [Google Scholar]

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