Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1994 Jun;96(3):427–431. doi: 10.1111/j.1365-2249.1994.tb06046.x

The significance of anti-streptokinase antibodies.

M Lynch 1, B L Pentecost 1, W A Littler 1, R A Stockley 1
PMCID: PMC1534565  PMID: 8004811

Abstract

Antibodies to streptokinase (SK) are widespread in the population, but reports of their effect on the action of SK are conflicting. Specific anti-SK IgG was purified from the sera of 10 patients, five with low titres of anti-SK IgG and five with high titres. The effect of increasing specific anti-SK IgG antibodies on the action of SK was evaluated in vitro using a fluorimetric assay for plasmin and by a fibrin plate lysis assay. The inhibition of SK by whole plasma from a further group of patients was also assessed by the fibrin plate assay. There was a positive correlation between the serum antibody concentration and the quantity of specific anti-SK eluted (r = 0.797; P < 0.005). The addition of specific anti-SK IgG caused a dose-related decrease in SK activity (fluorimetric assay r = -0.93; P = 0.02; fibrin plate assay r = -0.98; P < 0.001). The addition of patient plasma to the fibrin plate assay also resulted in decreased lysis, which was dependent upon antibody titre (r = -0.95; P < 0.0001). Significant in vitro reduction of the activity of SK by specific antibody was demonstrated, and this was similar with plasma containing comparable amounts of antibody. The findings suggest that treatment with SK would be unlikely to induce an effective thrombolytic state when antibody titres are high (such as those seen within 2 years of an initial dose of SK).

Full text

PDF
427

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bishop R., Ekert H., Gilchrist G., Shanbrom E., Fekete L. The preparation and evaluation of a standardized fibrin plate for the assessment of fibrinolytic activity. Thromb Diath Haemorrh. 1970 May 31;23(2):202–210. [PubMed] [Google Scholar]
  2. Deutsch D. G., Mertz E. T. Plasminogen: purification from human plasma by affinity chromatography. Science. 1970 Dec 4;170(3962):1095–1096. doi: 10.1126/science.170.3962.1095. [DOI] [PubMed] [Google Scholar]
  3. Fears R., Ferres H., Hibbs M., Standring R. Consequences of antibody binding in vitro on the pharmacological properties of anisoylated plasminogen streptokinase activator complex. Drugs. 1987;33 (Suppl 3):64–68. doi: 10.2165/00003495-198700333-00008. [DOI] [PubMed] [Google Scholar]
  4. Jalihal S., Morris G. K. Antistreptokinase titres after intravenous streptokinase. Lancet. 1990 Jan 27;335(8683):184–185. doi: 10.1016/0140-6736(90)90276-b. [DOI] [PubMed] [Google Scholar]
  5. Kaplan A. P. Unusual cold-induced disorders: cold-dependent dermatographism and systemic cold urticaria. J Allergy Clin Immunol. 1984 Apr;73(4):453–456. doi: 10.1016/0091-6749(84)90354-3. [DOI] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Lew A. S., Neer T., Rodriguez L., Geft I. L., Shah P. K., Ganz W. Clinical failure of streptokinase due to an unsuspected high titer of antistreptokinase antibody. J Am Coll Cardiol. 1984 Jul;4(1):183–185. doi: 10.1016/s0735-1097(84)80339-3. [DOI] [PubMed] [Google Scholar]
  8. Lynch M., Littler W. A., Pentecost B. L., Stockley R. A. Immunoglobulin response to intravenous streptokinase in acute myocardial infarction. Br Heart J. 1991 Aug;66(2):139–142. doi: 10.1136/hrt.66.2.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  10. Spöttl F., Kaiser R. Rapid detection and quantitation of precipitating streptokinase-antibodies. Thromb Diath Haemorrh. 1974 Dec 31;32(2-3):608–616. [PubMed] [Google Scholar]
  11. Summaria L., Arzadon L., Bernabe P., Robbins K. C. Studies on the isolation of the multiple molecular forms of human plasminogen and plasmin by isoelectric focusing methods. J Biol Chem. 1972 Jul 25;247(14):4691–4702. [PubMed] [Google Scholar]
  12. White H. D., Cross D. B., Williams B. F., Norris R. M. Safety and efficacy of repeat thrombolytic treatment after acute myocardial infarction. Br Heart J. 1990 Sep;64(3):177–181. doi: 10.1136/hrt.64.3.177. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES