Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1989 Oct;78(1):97–101.

Progressive ankylosis (ank/ank) in mice: an animal model of spondyloarthropathy. III. Proliferative spleen cell response to T cell mitogens.

H E Krug 1, M L Mahowald 1, C Clark 1
PMCID: PMC1534597  PMID: 2805429

Abstract

Murine progressive ankylosis is a spontaneous disorder of mice resulting from a homozygous recessive genetic defect (ank/ank) which produces an inflammatory arthritis of peripheral and axial joints eventually resulting in ankylosis of these joints. This disorder resembles the human spondyloarthropathies clinically, radiographically and histologically. Various studies in humans with spondyloarthropathies have described defects of cellular immunity but these results are conflicting. We measured the spleen cell response to mitogen in ank/ank mice and in normal littermates. The spleen cell response to the T cell mitogens phytohaemagglutinin and concanavalin A was decreased in ank/ank mice compared with their normal littermates. The response to the B cell mitogen lypopolysaccharide was normal in both ank/ank mice and normal littermates. Serum from ank/ank mice did not inhibit spleen cell responses to mitogen. Ank/ank spleen cells were not inhibitory of normal spleen cell responses to mitogens. Addition of irradiated normal spleen cells to ank/ank spleen cells did not restore the mitogen responses to normal. It is possible that the ank/ank gene results in the phenotypic expression of an abnormal or decreased cell product involved in T cell proliferation. Several recently described cytokines could be potential candidates for this product.

Full text

PDF
97

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aho K., Ahvonen P., Juvakoski T., Kousa M., Leirisalo M., Laitinen O. Immune responses in yersinia-associated reactive arthritis. Ann Rheum Dis. 1979;38 (Suppl 1):suppl–126. doi: 10.1136/ard.38.suppl_1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ball J. Articular pathology of ankylosing spondylitis. Clin Orthop Relat Res. 1979 Sep;(143):30–37. [PubMed] [Google Scholar]
  3. Clark C., LaSota I., Borch R. F. Evidence that X-irradiation and 4-hydroperoxycyclophosphamide affects different lymphocytes that respond to specific antigen in vitro. J Leukoc Biol. 1985 Feb;37(2):175–191. doi: 10.1002/jlb.37.2.175. [DOI] [PubMed] [Google Scholar]
  4. Dewhirst F. E., Stashenko P. P., Mole J. E., Tsurumachi T. Purification and partial sequence of human osteoclast-activating factor: identity with interleukin 1 beta. J Immunol. 1985 Oct;135(4):2562–2568. [PubMed] [Google Scholar]
  5. Ellingsworth L. R., Brennan J. E., Fok K., Rosen D. M., Bentz H., Piez K. A., Seyedin S. M. Antibodies to the N-terminal portion of cartilage-inducing factor A and transforming growth factor beta. Immunohistochemical localization and association with differentiating cells. J Biol Chem. 1986 Sep 15;261(26):12362–12367. [PubMed] [Google Scholar]
  6. Fan P. T., Clements P. J., Yu D. T., Opelz G., Bluestone R. Lymphocyte abnormalities in ankylosing spondylitis. Ann Rheum Dis. 1977 Oct;36(5):471–473. doi: 10.1136/ard.36.5.471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ford D. K. Yersinia-induced arthritis and Reiter's syndrome. Ann Rheum Dis. 1979;38 (Suppl 1):suppl–128. doi: 10.1136/ard.38.suppl_1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Froebel K., Sturrock R. D., Dick W. C., MacSween R. N. Cell-mediated immunity in the rheumatoid diseases. I. Skin testing and mitogenic responses in sero-negative arthritides. Clin Exp Immunol. 1975 Dec;22(3):446–452. [PMC free article] [PubMed] [Google Scholar]
  9. Geczy A. F., Alexander K., Bashir H. V., Edmonds J. P., Upfold L., Sullivan J. HLA-B27, Klebsiella and ankylosing spondylitis: biological and chemical studies. Immunol Rev. 1983;70:23–50. doi: 10.1111/j.1600-065x.1983.tb00708.x. [DOI] [PubMed] [Google Scholar]
  10. Goebel K. M., Goebel F. D., Baier R. Impaired cell-mediated immunity among HLA-B27 related rheumatoid variants responding to Yersinia antigen. J Clin Lab Immunol. 1982 Jun;8(2):75–81. [PubMed] [Google Scholar]
  11. Hart D. A., Finkelstein R. A. Inhibition of mitogen stimulation of human peripheral blood leukocytes by Vibrio cholerae enterotoxin. J Immunol. 1975 Jan;114(1 Pt 2):476–480. [PubMed] [Google Scholar]
  12. Mahowald M. L., Dalmasso A. P., Messner R. P. Endogenous inhibition of autologous lymphocyte antibody-dependent cellular cytotoxicity. Clin Immunol Immunopathol. 1983 Nov;29(2):211–222. doi: 10.1016/0090-1229(83)90025-9. [DOI] [PubMed] [Google Scholar]
  13. Mahowald M. L., Krug H., Halverson P. Progressive ankylosis (ank/ank) in mice: an animal model of spondyloarthropathy. II. Light and electron microscopic findings. J Rheumatol. 1989 Jan;16(1):60–66. [PubMed] [Google Scholar]
  14. Mahowald M. L., Krug H., Taurog J. Progressive ankylosis in mice. An animal model of spondylarthropathy. I. Clinical and radiographic findings. Arthritis Rheum. 1988 Nov;31(11):1390–1399. doi: 10.1002/art.1780311108. [DOI] [PubMed] [Google Scholar]
  15. Meryhew N. L., Zoschke D. C., Messner R. P. Anti-beta 2-microglobulin antibodies in systemic lupus erythematosus and ankylosing spondylitis: effects on in vitro lymphocyte function. J Rheumatol. 1986 Feb;13(1):83–89. [PubMed] [Google Scholar]
  16. Mikecz K., Glant T. T., Baron M., Poole A. R. Isolation of proteoglycan-specific T lymphocytes from patients with ankylosing spondylitis. Cell Immunol. 1988 Mar;112(1):55–63. doi: 10.1016/0008-8749(88)90275-4. [DOI] [PubMed] [Google Scholar]
  17. Mundy C. R., Altman A. J., Gondek M. D., Bandelin J. G. Direct resorption of bone by human monocytes. Science. 1977 Jun 3;196(4294):1109–1111. doi: 10.1126/science.16343. [DOI] [PubMed] [Google Scholar]
  18. Nawroth P. P., Bank I., Handley D., Cassimeris J., Chess L., Stern D. Tumor necrosis factor/cachectin interacts with endothelial cell receptors to induce release of interleukin 1. J Exp Med. 1986 Jun 1;163(6):1363–1375. doi: 10.1084/jem.163.6.1363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nikbin B., Brewerton D. A., James D. C., Hobbs J. R. Diminished mixed lymphocyte reaction in ankylosing spondylitis, relatives, and normal individuals all with HL-A 27. Ann Rheum Dis. 1976 Feb;35(1):37–39. doi: 10.1136/ard.35.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Resnick D. Radiology of seronegative spondyloarthropathies. Clin Orthop Relat Res. 1979 Sep;(143):38–45. [PubMed] [Google Scholar]
  21. Sheldon P. J., Pell P. A. Lymphocyte proliferative responses to bacterial antigens in B27-associated arthropathies. Br J Rheumatol. 1985 Feb;24(1):11–18. doi: 10.1093/rheumatology/24.1.11. [DOI] [PubMed] [Google Scholar]
  22. Sporn M. B., Roberts A. B., Wakefield L. M., Assoian R. K. Transforming growth factor-beta: biological function and chemical structure. Science. 1986 Aug 1;233(4763):532–534. doi: 10.1126/science.3487831. [DOI] [PubMed] [Google Scholar]
  23. Sweet H. O., Green M. C. Progressive ankylosis, a new skeletal mutation in the mouse. J Hered. 1981 Mar-Apr;72(2):87–93. doi: 10.1093/oxfordjournals.jhered.a109459. [DOI] [PubMed] [Google Scholar]
  24. Thomson B. M., Mundy G. R., Chambers T. J. Tumor necrosis factors alpha and beta induce osteoblastic cells to stimulate osteoclastic bone resorption. J Immunol. 1987 Feb 1;138(3):775–779. [PubMed] [Google Scholar]
  25. Treves S., Di Virgilio F., Cerundolo V., Zanovello P., Collavo D., Pozzan T. Calcium and inositolphosphates in the activation of T cell-mediated cytotoxicity. J Exp Med. 1987 Jul 1;166(1):33–42. doi: 10.1084/jem.166.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wee S. L., Daymond T. J. Diminished mixed lymphocyte response in ankylosing spondylitis. Tissue Antigens. 1978 May;11(5):409–417. doi: 10.1111/j.1399-0039.1978.tb01276.x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES