Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1990 Sep;81(3):412–416. doi: 10.1111/j.1365-2249.1990.tb05348.x

Role of interleukin-2 and interleukin-6 in the mitogen responsiveness of T cells from patients with 'common-variable' hypogammaglobulinaemia.

M E North 1, A D Webster 1, J Farrant 1
PMCID: PMC1534984  PMID: 2118844

Abstract

We have assessed the ability of interleukin-2 (IL-2) and interleukin-6 (IL-6) to augment the proliferative response of T lymphocytes from 'common-variable' hypogammaglobulinaemia (CVH) patients and from normal controls, to the mitogens phytohaemagglutinin (PHA) and OKT3. We show that with cells from the control group and from those patients whose T cells respond to PHA within the control range, both IL-2 and IL-6 will significantly augment the response to OKT3. However, in those patients with a T cell defect in which the PHA response is below the control range, neither IL-2 nor IL-6 could restore the PHA or OKT3 response to normal. Responses to IL-2 or IL-6 alone were always in or above the control range.

Full text

PDF
412

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson U., Sander B., Andersson J., Möller G. Concomitant production of different lymphokines in activated T cells. Eur J Immunol. 1988 Dec;18(12):2081–2084. doi: 10.1002/eji.1830181232. [DOI] [PubMed] [Google Scholar]
  2. Cantrell D. A., Collins M. K., Crumpton M. J. Autocrine regulation of T-lymphocyte proliferation: differential induction of IL-2 and IL-2 receptor. Immunology. 1988 Nov;65(3):343–349. [PMC free article] [PubMed] [Google Scholar]
  3. Cantrell D. A., Smith K. A. The interleukin-2 T-cell system: a new cell growth model. Science. 1984 Jun 22;224(4655):1312–1316. doi: 10.1126/science.6427923. [DOI] [PubMed] [Google Scholar]
  4. Ceuppens J. L., Baroja M. L., Lorre K., Van Damme J., Billiau A. Human T cell activation with phytohemagglutinin. The function of IL-6 as an accessory signal. J Immunol. 1988 Dec 1;141(11):3868–3874. [PubMed] [Google Scholar]
  5. Chatila T. A., Schwartz D. H., Miller R., Geha R. S. Requirement for mitogen, T cell-accessory cell contact, and interleukin 1 in the induction of resting T-cell proliferation. Clin Immunol Immunopathol. 1987 Aug;44(2):235–247. doi: 10.1016/0090-1229(87)90068-7. [DOI] [PubMed] [Google Scholar]
  6. Cunningham-Rundles C. Clinical and immunologic analyses of 103 patients with common variable immunodeficiency. J Clin Immunol. 1989 Jan;9(1):22–33. doi: 10.1007/BF00917124. [DOI] [PubMed] [Google Scholar]
  7. Davis L., Lipsky P. E. Signals involved in T cell activation. II. Distinct roles of intact accessory cells, phorbol esters, and interleukin 1 in activation and cell cycle progression of resting T lymphocytes. J Immunol. 1986 May 15;136(10):3588–3596. [PubMed] [Google Scholar]
  8. Eibl M. M., Mannhalter J. W., Zlabinger G., Mayr W. R., Tilz G. P., Ahmad R., Zielinski C. C. Defective macrophage function in a patient with common variable immunodeficiency. N Engl J Med. 1982 Sep 23;307(13):803–806. doi: 10.1056/NEJM198209233071307. [DOI] [PubMed] [Google Scholar]
  9. Farrant J., Clark J. C., Lee H., Knight S. C., O'Brien J. Conditions for measuring DNA synthesis in PHA stimulated human lymphocytes in 20 microliters hanging drops with various cell concentrations and periods of culture. J Immunol Methods. 1980;33(4):301–312. doi: 10.1016/0022-1759(80)90001-0. [DOI] [PubMed] [Google Scholar]
  10. Farrant J., Newton C. A., North M. E., Weyman C., Brenner M. K. Production of antibody by human B cells under serum-free conditions. J Immunol Methods. 1984 Mar 30;68(1-2):25–34. doi: 10.1016/0022-1759(84)90133-9. [DOI] [PubMed] [Google Scholar]
  11. Fiedler W., Sykora K. W., Welte K., Kolitz J. E., Cunningham-Rundles C., Holloway K., Miller G. A., Souza L., Mertelsmann R. T-cell activation defect in common variable immunodeficiency: restoration by phorbol myristate acetate (PMA) or allogeneic macrophages. Clin Immunol Immunopathol. 1987 Aug;44(2):206–218. doi: 10.1016/0090-1229(87)90066-3. [DOI] [PubMed] [Google Scholar]
  12. Habetswallner D., Pelosi E., Bulgarini D., Camagna A., Samoggia P., Montesoro E., Giannella G., Lazzaro D., Isacchi G., Testa U. Activation and proliferation of normal resting human T lymphocytes in serum-free culture: role of IL-4 and IL-6. Immunology. 1988 Nov;65(3):357–364. [PMC free article] [PubMed] [Google Scholar]
  13. Halvorsen R., Leivestad T., Gaudernack G., Thorsby E. Role of accessory cells in the activation of pure T cells via the T cell receptor-CD3 complex or with phytohaemagglutinin. Scand J Immunol. 1988 May;27(5):555–563. doi: 10.1111/j.1365-3083.1988.tb02383.x. [DOI] [PubMed] [Google Scholar]
  14. Houssiau F. A., Coulie P. G., Olive D., Van Snick J. Synergistic activation of human T cells by interleukin 1 and interleukin 6. Eur J Immunol. 1988 Apr;18(4):653–656. doi: 10.1002/eji.1830180427. [DOI] [PubMed] [Google Scholar]
  15. Kruger G., Welte K., Ciobanu N., Cunningham-Rundles C., Ralph P., Venuta S., Feldman S., Koziner B., Wang C. Y., Moore M. A. Interleukin-2 correction of defective in vitro T-cell mitogenesis in patients with common varied immunodeficiency. J Clin Immunol. 1984 Jul;4(4):295–303. doi: 10.1007/BF00915297. [DOI] [PubMed] [Google Scholar]
  16. Le J. M., Fredrickson G., Reis L. F., Diamantstein T., Hirano T., Kishimoto T., Vilcek J. Interleukin 2-dependent and interleukin 2-independent pathways of regulation of thymocyte function by interleukin 6. Proc Natl Acad Sci U S A. 1988 Nov;85(22):8643–8647. doi: 10.1073/pnas.85.22.8643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. López-Botet M., Fontán G., Garcia Rodriguez M. C., de Landázuri M. O. Relationship between IL 2 synthesis and the proliferative response to PHA in different primary immunodeficiencies. J Immunol. 1982 Feb;128(2):679–683. [PubMed] [Google Scholar]
  18. North M. E., Spickett G. P., Allsop J., Webster A. D., Farrant J. Defective DNA synthesis by T cells in acquired 'common-variable' hypogammaglobulinaemia on stimulation with mitogens. Clin Exp Immunol. 1989 Apr;76(1):19–23. [PMC free article] [PubMed] [Google Scholar]
  19. Umetsu D. T., Katzen D., Chatila T., Miller R., Jabara H. H., Maher M., Oettgen H., Terhorst C., Geha R. S. Requirements for activation of human peripheral blood T cells by mouse monoclonal antibodies to CD3. Clin Immunol Immunopathol. 1987 Apr;43(1):48–64. doi: 10.1016/0090-1229(87)90156-5. [DOI] [PubMed] [Google Scholar]
  20. Webster A. D., Asherson G. L. Identification and function of T cells in the peripheral blood of patients with hypogammaglobulinaemia. Clin Exp Immunol. 1974 Dec;18(4):499–504. [PMC free article] [PubMed] [Google Scholar]
  21. de Vries J. E., Caviles A. P., Jr, Bont W. S., Mendelsohn J. The role of monocytes in human lymphocyte activation by mitogens. J Immunol. 1979 Mar;122(3):1099–1107. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES