Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1990 Jul;81(1):123–126. doi: 10.1111/j.1365-2249.1990.tb05301.x

Differential expression of the calcium-binding proteins MRP8 and MRP14 in granulomatous conditions: an immunohistochemical study.

J Delabie 1, C de Wolf-Peeters 1, J J van den Oord 1, V J Desmet 1
PMCID: PMC1535019  PMID: 2199095

Abstract

MRP14 and MRP8 are well-characterized calcium-binding proteins present in myeloid cells and mononuclear phagocytes. These antigens can easily be visualized in paraffin-embedded tissue, making use of monospecific polyclonal antibodies. This study evaluates MRP14 and MRP8 expression in mononuclear phagocytes in various granulomatous conditions. MRP14 is strongly expressed in all granulomatous conditions. MRP8 is variably expressed. Mononuclear phagocytes in granulomas of foreign body type, cat-scratch disease and erythema nodosum strongly express MRP8. In contrast, MRP8 expression is weak or absent in mononuclear phagocytes of sarcoidosis and tuberculosis. These results show differences in immunophenotype between non-phagocytic mononuclear phagocytes in delayed hypersensitivity type granulomas and phagocytic mononuclear phagocytes in non-hypersensitivity and non-immunological granulomas.

Full text

PDF
123

Images in this article

124Fig. 1
on p.124
125Fig. 2
on p.125

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams T. E., Bodmer J. G., Bodmer W. F. Production and characterization of monoclonal antibodies recognizing the alpha-chain subunits of human ia alloantigens. Immunology. 1983 Dec;50(4):613–624. [PMC free article] [PubMed] [Google Scholar]
  2. Brandtzaeg P., Dale I., Fagerhol M. K. Distribution of a formalin-resistant myelomonocytic antigen (L1) in human tissues. I. Comparison with other leukocyte markers by paired immunofluorescence and immunoenzyme staining. Am J Clin Pathol. 1987 Jun;87(6):681–699. doi: 10.1093/ajcp/87.6.681. [DOI] [PubMed] [Google Scholar]
  3. Dale I., Brandtzaeg P., Fagerhol M. K., Scott H. Distribution of a new myelomonocytic antigen (L1) in human peripheral blood leukocytes. Immunofluorescence and immunoperoxidase staining features in comparison with lysozyme and lactoferrin. Am J Clin Pathol. 1985 Jul;84(1):24–34. doi: 10.1093/ajcp/84.1.24. [DOI] [PubMed] [Google Scholar]
  4. Flavell D. J., Jones D. B., Wright D. H. Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J Histochem Cytochem. 1987 Nov;35(11):1217–1226. doi: 10.1177/35.11.3309045. [DOI] [PubMed] [Google Scholar]
  5. Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
  6. Kaufmann S. H., Flesch I. E. The role of T cell--macrophage interactions in tuberculosis. Springer Semin Immunopathol. 1988;10(4):337–358. doi: 10.1007/BF02053845. [DOI] [PubMed] [Google Scholar]
  7. Munro C. S., Campbell D. A., Collings L. A., Poulter L. W. Monoclonal antibodies distinguish macrophages and epithelioid cells in sarcoidosis and leprosy. Clin Exp Immunol. 1987 May;68(2):282–287. [PMC free article] [PubMed] [Google Scholar]
  8. Niemi K. M., Förström L., Hannuksela M., Mustakallio K. K., Salo O. P. Nodules on the legs. A clinical, histological and immunohistological study of 82 patients representing different types of nodular panniculitis. Acta Derm Venereol. 1977;57(2):145–154. [PubMed] [Google Scholar]
  9. Odink K., Cerletti N., Brüggen J., Clerc R. G., Tarcsay L., Zwadlo G., Gerhards G., Schlegel R., Sorg C. Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis. Nature. 1987 Nov 5;330(6143):80–82. doi: 10.1038/330080a0. [DOI] [PubMed] [Google Scholar]
  10. Poulter L. W., Campbell D. A., Munro C., Janossy G. Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies. Scand J Immunol. 1986 Sep;24(3):351–357. doi: 10.1111/j.1365-3083.1986.tb02104.x. [DOI] [PubMed] [Google Scholar]
  11. Pulford K. A., Rigney E. M., Micklem K. J., Jones M., Stross W. P., Gatter K. C., Mason D. Y. KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol. 1989 Apr;42(4):414–421. doi: 10.1136/jcp.42.4.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Semenzato G. Recent advances on sarcoid immunology. Sarcoidosis. 1988 Mar;5(1):5–7. [PubMed] [Google Scholar]
  13. Spector W. G., Heesom N. The production of granulomata by antigen-antibody complexes. J Pathol. 1969 May;98(1):31–39. doi: 10.1002/path.1710980105. [DOI] [PubMed] [Google Scholar]
  14. Warren K. S. A functional classification of granulomatous inflammation. Ann N Y Acad Sci. 1976;278:7–18. doi: 10.1111/j.1749-6632.1976.tb47011.x. [DOI] [PubMed] [Google Scholar]
  15. Wear D. J., Margileth A. M., Hadfield T. L., Fischer G. W., Schlagel C. J., King F. M. Cat scratch disease: a bacterial infection. Science. 1983 Sep 30;221(4618):1403–1405. doi: 10.1126/science.6612349. [DOI] [PubMed] [Google Scholar]
  16. van den Oprd J. J., de Wolf-Peeters C., Facchetti F., Desmet V. J. Cellular composition of hypersensitivity-type granulomas: immunohistochemical analysis of tuberculous and sarcoidal lymphadenitis. Hum Pathol. 1984 Jun;15(6):559–565. doi: 10.1016/s0046-8177(84)80010-6. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES