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. 1990 Aug;81(2):256–262. doi: 10.1111/j.1365-2249.1990.tb03327.x

Recognition of synthetic peptides of Sm-D autoantigen by lupus sera.

S Barakat 1, J P Briand 1, J C Weber 1, M H van Regenmortel 1, S Muller 1
PMCID: PMC1535060  PMID: 2387092

Abstract

The reactivity of autoantibodies present in the serum of patients with systemic lupus erythematosus (SLE) was investigated by ELISA using seven overlapping synthetic peptides representing the entire sequence of the polypeptide D component of 'Sm antigen'. Of the 165 SLE sera tested, 59% were found to contain IgG antibodies able to bind to peptide 1-20, while 37% of the sera reacted with peptide 44-67. All sera reacting with peptide 44-67 also reacted with peptide 1-20. These two peptides were only seldom recognized by the sera of 187 patients with other rheumatic autoimmune diseases or by 53 sera of normal individuals. In a parallel study using sera that reacted with the D band in immunoblotting, most of the sera recognized peptides 44-67 (89%) and 1-20 (67%), while 33% of them reacted with peptide 97-119. The use of these synthetic peptides in ELISA may be of considerable help for detecting anti Sm autoantibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Billings P. B., Allen R. W., Jensen F. C., Hoch S. O. Anti-RNP monoclonal antibodies derived from a mouse strain with lupus-like autoimmunity. J Immunol. 1982 Mar;128(3):1176–1180. [PubMed] [Google Scholar]
  2. Billings P. B., Barton J. R., Hoch S. O. A murine monoclonal antibody recognizes the 13,000 molecular weight polypeptide of the Sm small nuclear ribonucleoprotein complex. J Immunol. 1985 Jul;135(1):428–432. [PubMed] [Google Scholar]
  3. Bringmann P., Lührmann R. Purification of the individual snRNPs U1, U2, U5 and U4/U6 from HeLa cells and characterization of their protein constituents. EMBO J. 1986 Dec 20;5(13):3509–3516. doi: 10.1002/j.1460-2075.1986.tb04676.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Combe B., Rucheton M., Graafland H., Lussiez V., Brunel C., Sany J. Clinical significance of anti-RNP and anti-Sm autoantibodies as determined by immunoblotting and immunoprecipitation in sera from patients with connective tissue diseases. Clin Exp Immunol. 1989 Jan;75(1):18–24. [PMC free article] [PubMed] [Google Scholar]
  5. Eisenberg R. A., Dyer K., Craven S. Y., Fuller C. R., Yount W. J. Subclass restriction and polyclonality of the systemic lupus erythematosus marker antibody anti-Sm. J Clin Invest. 1985 Apr;75(4):1270–1277. doi: 10.1172/JCI111826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Habets W. J., Sillekens P. T., Hoet M. H., Schalken J. A., Roebroek A. J., Leunissen J. A., van de Ven W. J., van Venrooij W. J. Analysis of a cDNA clone expressing a human autoimmune antigen: full-length sequence of the U2 small nuclear RNA-associated B" antigen. Proc Natl Acad Sci U S A. 1987 Apr;84(8):2421–2425. doi: 10.1073/pnas.84.8.2421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hardin J. A. The lupus autoantigens and the pathogenesis of systemic lupus erythematosus. Arthritis Rheum. 1986 Apr;29(4):457–460. doi: 10.1002/art.1780290401. [DOI] [PubMed] [Google Scholar]
  8. Hopp T. P., Woods K. R. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824–3828. doi: 10.1073/pnas.78.6.3824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaiser E., Colescott R. L., Bossinger C. D., Cook P. I. Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides. Anal Biochem. 1970 Apr;34(2):595–598. doi: 10.1016/0003-2697(70)90146-6. [DOI] [PubMed] [Google Scholar]
  10. Lerner M. R., Steitz J. A. Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5495–5499. doi: 10.1073/pnas.76.11.5495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McCarty G. A., Rice J. R., Bembe M. L., Pisetsky D. S. Independent expression of autoantibodies in systemic lupus erythematosus. J Rheumatol. 1982 Sep-Oct;9(5):691–695. [PubMed] [Google Scholar]
  12. Muller S., Bonnier D., Thiry M., Van Regenmortel M. H. Reactivity of autoantibodies in systemic lupus erythematosus with synthetic core histone peptides. Int Arch Allergy Appl Immunol. 1989;89(2-3):288–296. doi: 10.1159/000234962. [DOI] [PubMed] [Google Scholar]
  13. Muller S., Briand J. P., Van Regenmortel M. H. Presence of antibodies to ubiquitin during the autoimmune response associated with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1988 Nov;85(21):8176–8180. doi: 10.1073/pnas.85.21.8176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Muller S., Plaue S., Couppez M., Van Regenmortel M. H. Comparison of different methods for localizing antigenic regions in histone H2A. Mol Immunol. 1986 Jun;23(6):593–601. doi: 10.1016/0161-5890(86)90095-7. [DOI] [PubMed] [Google Scholar]
  15. Parker J. M., Guo D., Hodges R. S. New hydrophilicity scale derived from high-performance liquid chromatography peptide retention data: correlation of predicted surface residues with antigenicity and X-ray-derived accessible sites. Biochemistry. 1986 Sep 23;25(19):5425–5432. doi: 10.1021/bi00367a013. [DOI] [PubMed] [Google Scholar]
  16. Plaué S., Muller S., van Regenmortel M. H. A branched, synthetic octapeptide of ubiquitinated histone H2A as target of autoantibodies. J Exp Med. 1989 May 1;169(5):1607–1617. doi: 10.1084/jem.169.5.1607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reuter R., Lührmann R. Immunization of mice with purified U1 small nuclear ribonucleoprotein (RNP) induces a pattern of antibody specificities characteristic of the anti-Sm and anti-RNP autoimmune response of patients with lupus erythematosus, as measured by monoclonal antibodies. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8689–8693. doi: 10.1073/pnas.83.22.8689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rokeach L. A., Haselby J. A., Hoch S. O. Molecular cloning of a cDNA encoding the human Sm-D autoantigen. Proc Natl Acad Sci U S A. 1988 Jul;85(13):4832–4836. doi: 10.1073/pnas.85.13.4832. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rokeach L. A., Jannatipour M., Haselby J. A., Hoch S. O. Primary structure of a human small nuclear ribonucleoprotein polypeptide as deduced by cDNA analysis. J Biol Chem. 1989 Mar 25;264(9):5024–5030. [PubMed] [Google Scholar]
  20. Sillekens P. T., Beijer R. P., Habets W. J., van Verooij W. J. Molecular cloning of the cDNA for the human U2 snRNA-specific A' protein. Nucleic Acids Res. 1989 Mar 11;17(5):1893–1906. doi: 10.1093/nar/17.5.1893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sillekens P. T., Habets W. J., Beijer R. P., van Venrooij W. J. cDNA cloning of the human U1 snRNA-associated A protein: extensive homology between U1 and U2 snRNP-specific proteins. EMBO J. 1987 Dec 1;6(12):3841–3848. doi: 10.1002/j.1460-2075.1987.tb02721.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stanford D. R., Rohleder A., Neiswanger K., Wieben E. D. DNA sequence of a human Sm autoimmune antigen. The multigene family contains a processed pseudogene. J Biol Chem. 1987 Jul 25;262(21):9931–9934. [PubMed] [Google Scholar]
  23. Tan E. M., Chan E. K., Sullivan K. F., Rubin R. L. Antinuclear antibodies (ANAs): diagnostically specific immune markers and clues toward the understanding of systemic autoimmunity. Clin Immunol Immunopathol. 1988 May;47(2):121–141. doi: 10.1016/0090-1229(88)90066-9. [DOI] [PubMed] [Google Scholar]
  24. Tan E. M., Kunkel H. G. Characteristics of a soluble nuclear antigen precipitating with sera of patients with systemic lupus erythematosus. J Immunol. 1966 Mar;96(3):464–471. [PubMed] [Google Scholar]
  25. Theissen H., Etzerodt M., Reuter R., Schneider C., Lottspeich F., Argos P., Lührmann R., Philipson L. Cloning of the human cDNA for the U1 RNA-associated 70K protein. EMBO J. 1986 Dec 1;5(12):3209–3217. doi: 10.1002/j.1460-2075.1986.tb04631.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Williams D. G., Charles P. J., Maini R. N. Preparative isolation of p67, A, B, B' and D from nRNP/Sm and Sm antigens by reverse-phase chromatography. Use in a polypeptide-specific ELISA for independent quantitation of anti-nRNP and anti-Sm antibodies. J Immunol Methods. 1988 Oct 4;113(1):25–35. doi: 10.1016/0022-1759(88)90378-x. [DOI] [PubMed] [Google Scholar]
  27. Yamamoto K., Miura H., Moroi Y., Yoshinoya S., Goto M., Nishioka K., Miyamoto T. Isolation and characterization of a complementary DNA expressing human U1 small nuclear ribonucleoprotein C polypeptide. J Immunol. 1988 Jan 1;140(1):311–317. [PubMed] [Google Scholar]

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