Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1990 Oct;82(1):10–15. doi: 10.1111/j.1365-2249.1990.tb05396.x

Reduced oxidative burst responses in monocytes and monocyte-derived macrophages from HIV-infected subjects.

F Müller 1, H Rollag 1, S S Frøland 1
PMCID: PMC1535145  PMID: 1976461

Abstract

Oxidative burst responses of monocytes and monocyte-derived macrophages (MDM) were studied in 40 subjects with HIV infection of different clinical stages. Oxidative burst was assessed as reduction of nitroblue tetrazolium (NBT) with or without stimulants. Results were determined as oxidative burst responses per cell and as a stimulatory ratio between stimulated and unstimulated NBT reduction. Cells from 12 HIV-seronegative homosexual men and 38 blood donors served as control groups. In patients with asymptomatic HIV infection, monocyte oxidative burst responses were reduced compared with the blood donors. In MDM from the same patients, stimulatory ratios were reduced. In AIDS patients, stimulatory ratios of both monocytes and MDM were reduced compared with controls. In contrast to the progressive deterioration of CD4+ lymphocyte counts as well as other immune functions in HIV infection, monocyte oxidative burst responses are impaired already in the asymptomatic phase of the infection, almost to the same extent as in patients with AIDS.

Full text

PDF
10

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bender B. S., Davidson B. L., Kline R., Brown C., Quinn T. C. Role of the mononuclear phagocyte system in the immunopathogenesis of human immunodeficiency virus infection and the acquired immunodeficiency syndrome. Rev Infect Dis. 1988 Nov-Dec;10(6):1142–1154. doi: 10.1093/clinids/10.6.1142. [DOI] [PubMed] [Google Scholar]
  2. Braun D. P., Kessler H., Falk L., Paul D., Harris J. E., Blaauw B., Landay A. Monocyte functional studies in asymptomatic, human immunodeficiency disease virus (HIV)-infected individuals. J Clin Immunol. 1988 Nov;8(6):486–494. doi: 10.1007/BF00916955. [DOI] [PubMed] [Google Scholar]
  3. Breen E. C., Rezai A. R., Nakajima K., Beall G. N., Mitsuyasu R. T., Hirano T., Kishimoto T., Martinez-Maza O. Infection with HIV is associated with elevated IL-6 levels and production. J Immunol. 1990 Jan 15;144(2):480–484. [PubMed] [Google Scholar]
  4. Brinchmann J. E., Vartdal F., Gaudernack G., Markussen G., Funderud S., Ugelstad J., Thorsby E. Direct immunomagnetic quantification of lymphocyte subsets in blood. Clin Exp Immunol. 1988 Jan;71(1):182–186. [PMC free article] [PubMed] [Google Scholar]
  5. Brinchmann J. E., Vartdal F., Thorsby E. T lymphocyte subset changes in human immunodeficiency virus infection. J Acquir Immune Defic Syndr. 1989;2(4):398–403. [PubMed] [Google Scholar]
  6. Eales L. J., Moshtael O., Pinching A. J. Microbicidal activity of monocyte derived macrophages in AIDS and related disorders. Clin Exp Immunol. 1987 Feb;67(2):227–235. [PMC free article] [PubMed] [Google Scholar]
  7. Estevez M. E., Ballart I. J., Diez R. A., Planes N., Scaglione C., Sen L. Early defect of phagocytic cell function in subjects at risk for acquired immunodeficiency syndrome. Scand J Immunol. 1986 Aug;24(2):215–221. doi: 10.1111/j.1365-3083.1986.tb02088.x. [DOI] [PubMed] [Google Scholar]
  8. Fauci A. S. The human immunodeficiency virus: infectivity and mechanisms of pathogenesis. Science. 1988 Feb 5;239(4840):617–622. doi: 10.1126/science.3277274. [DOI] [PubMed] [Google Scholar]
  9. Gartner S., Markovits P., Markovitz D. M., Kaplan M. H., Gallo R. C., Popovic M. The role of mononuclear phagocytes in HTLV-III/LAV infection. Science. 1986 Jul 11;233(4760):215–219. doi: 10.1126/science.3014648. [DOI] [PubMed] [Google Scholar]
  10. Harrell R. A., Cianciolo G. J., Copeland T. D., Oroszlan S., Snyderman R. Suppression of the respiratory burst of human monocytes by a synthetic peptide homologous to envelope proteins of human and animal retroviruses. J Immunol. 1986 May 15;136(10):3517–3520. [PubMed] [Google Scholar]
  11. Ho D. D., Rota T. R., Hirsch M. S. Infection of monocyte/macrophages by human T lymphotropic virus type III. J Clin Invest. 1986 May;77(5):1712–1715. doi: 10.1172/JCI112491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Levy J. A., Shimabukuro J., McHugh T., Casavant C., Stites D., Oshiro L. AIDS-associated retroviruses (ARV) can productively infect other cells besides human T helper cells. Virology. 1985 Dec;147(2):441–448. doi: 10.1016/0042-6822(85)90146-1. [DOI] [PubMed] [Google Scholar]
  13. Maehlen T., Degré M. Lack of activity of fusidic acid against human immunodeficiency virus in monocytes. Antimicrob Agents Chemother. 1989 May;33(5):680–683. doi: 10.1128/aac.33.5.680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Murray H. W., Gellene R. A., Libby D. M., Rothermel C. D., Rubin B. Y. Activation of tissue macrophages from AIDS patients: in vitro response of AIDS alveolar macrophages to lymphokines and interferon-gamma. J Immunol. 1985 Oct;135(4):2374–2377. [PubMed] [Google Scholar]
  15. Müller F., Frøland S. S., Brandtzaeg P. Altered IgG-subclass distribution in lymph node cells and serum of adults infected with human immunodeficiency virus (HIV). Clin Exp Immunol. 1989 Nov;78(2):153–158. [PMC free article] [PubMed] [Google Scholar]
  16. Müller F., Rollag H., Frøland S. S. Nitroblue tetrazolium reduction in monocytes and monocyte-derived macrophages. Effect of oxidative burst stimulants and interferons. APMIS. 1989 Jun;97(6):490–496. doi: 10.1111/j.1699-0463.1989.tb00821.x. [DOI] [PubMed] [Google Scholar]
  17. Müller F., Rollag H., Gaudernack G., Frøland S. S. Impaired in vitro survival of monocytes from patients with HIV infection. Clin Exp Immunol. 1990 Jul;81(1):25–30. doi: 10.1111/j.1365-2249.1990.tb05286.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nicholson J. K., Cross G. D., Callaway C. S., McDougal J. S. In vitro infection of human monocytes with human T lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV). J Immunol. 1986 Jul 1;137(1):323–329. [PubMed] [Google Scholar]
  19. Nielsen H., Kharazmi A., Faber V. Blood monocyte and neutrophil functions in the acquired immune deficiency syndrome. Scand J Immunol. 1986 Sep;24(3):291–296. doi: 10.1111/j.1365-3083.1986.tb02096.x. [DOI] [PubMed] [Google Scholar]
  20. Parks D. R., Bryan V. M., Oi V. T., Herzenberg L. A. Antigen-specific identification and cloning of hybridomas with a fluorescence-activated cell sorter. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1962–1966. doi: 10.1073/pnas.76.4.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pick E., Charon J., Mizel D. A rapid densitometric microassay for nitroblue tetrazolium reduction and application of the microassay to macrophages. J Reticuloendothel Soc. 1981 Dec;30(6):581–593. [PubMed] [Google Scholar]
  22. Poli G., Bottazzi B., Acero R., Bersani L., Rossi V., Introna M., Lazzarin A., Galli M., Mantovani A. Monocyte function in intravenous drug abusers with lymphadenopathy syndrome and in patients with acquired immunodeficiency syndrome: selective impairment of chemotaxis. Clin Exp Immunol. 1985 Oct;62(1):136–142. [PMC free article] [PubMed] [Google Scholar]
  23. Rook G. A., Steele J., Umar S., Dockrell H. M. A simple method for the solubilisation of reduced NBT, and its use as a colorimetric assay for activation of human macrophages by gamma-interferon. J Immunol Methods. 1985 Sep 3;82(1):161–167. doi: 10.1016/0022-1759(85)90235-2. [DOI] [PubMed] [Google Scholar]
  24. Roux-Lombard P., Aladjem D., Balavoine J. F., Chofflon M., Despont J. P., Hirschel B., Jeannet M., Kapanci Y., Lang R., Toccanier M. F. Altered functions of peripheral blood monocytes in homosexual males and intravenous drug users with persistent generalized lymphadenopathy. Eur J Clin Invest. 1986 Jun;16(3):262–270. doi: 10.1111/j.1365-2362.1986.tb01340.x. [DOI] [PubMed] [Google Scholar]
  25. Salahuddin S. Z., Rose R. M., Groopman J. E., Markham P. D., Gallo R. C. Human T lymphotropic virus type III infection of human alveolar macrophages. Blood. 1986 Jul;68(1):281–284. [PubMed] [Google Scholar]
  26. Smith P. D., Ohura K., Masur H., Lane H. C., Fauci A. S., Wahl S. M. Monocyte function in the acquired immune deficiency syndrome. Defective chemotaxis. J Clin Invest. 1984 Dec;74(6):2121–2128. doi: 10.1172/JCI111637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Spear G. T., Kessler H. A., Rothberg L., Phair J., Landay A. L. Decreased oxidative burst activity of monocytes from asymptomatic HIV-infected individuals. Clin Immunol Immunopathol. 1990 Feb;54(2):184–191. doi: 10.1016/0090-1229(90)90080-a. [DOI] [PubMed] [Google Scholar]
  28. Stella C. C., Ganser A., Hoelzer D. Defective in vitro growth of the hemopoietic progenitor cells in the acquired immunodeficiency syndrome. J Clin Invest. 1987 Aug;80(2):286–293. doi: 10.1172/JCI113071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Unkeless J. C., Eisen H. N. Binding of monomeric immunoglobulins to Fc receptors of mouse macrophages. J Exp Med. 1975 Dec 1;142(6):1520–1533. doi: 10.1084/jem.142.6.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Washburn R. G., Tuazon C. U., Bennett J. E. Phagocytic and fungicidal activity of monocytes from patients with acquired immunodeficiency syndrome. J Infect Dis. 1985 Mar;151(3):565–566. doi: 10.1093/infdis/151.3.565. [DOI] [PubMed] [Google Scholar]
  31. Wright S. C., Jewett A., Mitsuyasu R., Bonavida B. Spontaneous cytotoxicity and tumor necrosis factor production by peripheral blood monocytes from AIDS patients. J Immunol. 1988 Jul 1;141(1):99–104. [PubMed] [Google Scholar]
  32. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES