Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1990 May;80(2):257–262. doi: 10.1111/j.1365-2249.1990.tb05244.x

Augmentation of the human monocyte/macrophage chemiluminescence response during short-term exposure to interferon-gamma and tumour necrosis factor-alpha.

L M Kumaratilake 1, A Ferrante 1, E J Bates 1, I C Kowanko 1
PMCID: PMC1535285  PMID: 2113442

Abstract

The effects of short-term (30 min) pre-incubation of human monocytes and macrophages (3-day cultured monocytes) with leucocyte-derived human interferon-gamma (IFN-gamma) and recombinant human tumour necrosis factor-alpha (rTNF-alpha) were examined. Pre-incubation of either monocytes or macrophages with rTNF-alpha or IFN-gamma (100 U/5 x 10(5) cells) augmented their respiratory burst to formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), measured by the luminol- and lucigenin-dependent chemiluminescence assay. In addition, both cell types showed a burst of respiratory activity in the presence of rTNF-alpha or IFN-gamma only. The effects of IFN-gamma were removed by adsorption with an anti-IFN-gamma monoclonal antibody and those of rTNF-alpha were abolished by heating at 100 degrees C, or by the addition of anti-TNF-alpha monoclonal antibody. The results demonstrate that both IFN-gamma and rTNF-alpha are stimulators of monocytes and macrophages, and rapidly alter the capacity of the cells to respond to fMLP, which binds to cell surface receptors.

Full text

PDF
257

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bates E. J., Kowanko I. C., Ferrante A. Conditioned medium from stimulated mononuclear leucocytes potentiates the ability of human neutrophils to damage human articular cartilage. Ann Rheum Dis. 1988 Jan;47(1):1–9. doi: 10.1136/ard.47.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buchan G., Barrett K., Turner M., Chantry D., Maini R. N., Feldmann M. Interleukin-1 and tumour necrosis factor mRNA expression in rheumatoid arthritis: prolonged production of IL-1 alpha. Clin Exp Immunol. 1988 Sep;73(3):449–455. [PMC free article] [PubMed] [Google Scholar]
  3. Cassatella M. A., Della Bianca V., Berton G., Rossi F. Activation by gamma interferon of human macrophage capability to produce toxic oxygen molecules is accompanied by decreased Km of the superoxide-generating NADPH oxidase. Biochem Biophys Res Commun. 1985 Nov 15;132(3):908–914. doi: 10.1016/0006-291x(85)91893-5. [DOI] [PubMed] [Google Scholar]
  4. De Titto E. H., Catterall J. R., Remington J. S. Activity of recombinant tumor necrosis factor on Toxoplasma gondii and Trypanosoma cruzi. J Immunol. 1986 Aug 15;137(4):1342–1345. [PubMed] [Google Scholar]
  5. DeGré M., Mellbye O. J., Clarke-Jenssen O. Immune interferon in serum and synovial fluid in rheumatoid arthritis and related disorders. Ann Rheum Dis. 1983 Dec;42(6):672–676. doi: 10.1136/ard.42.6.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ding A. H., Nathan C. F. Trace levels of bacterial lipopolysaccharide prevent interferon-gamma or tumor necrosis factor-alpha from enhancing mouse peritoneal macrophage respiratory burst capacity. J Immunol. 1987 Sep 15;139(6):1971–1977. [PubMed] [Google Scholar]
  7. Esparza I., Ruppel A., Mestan J., Krammer P. H. Preactivation of macrophages in mice acutely infected with Schistosoma mansoni. Immunobiology. 1988 May;177(2):105–119. doi: 10.1016/S0171-2985(88)80032-9. [DOI] [PubMed] [Google Scholar]
  8. Feinman R., Siegel D. S., Le J. M., Vilcek J. Interferon-gamma enhances target cell sensitivity to monocyte killing. Cell Immunol. 1986 Apr 15;99(1):287–293. doi: 10.1016/0008-8749(86)90236-4. [DOI] [PubMed] [Google Scholar]
  9. Ferrante A., Beard L. J. IgG subclass assays with polyclonal antisera and monoclonal antibodies. Monogr Allergy. 1988;23:61–72. [PubMed] [Google Scholar]
  10. Ferrante A., Nandoskar M., Walz A., Goh D. H., Kowanko I. C. Effects of tumour necrosis factor alpha and interleukin-1 alpha and beta on human neutrophil migration, respiratory burst and degranulation. Int Arch Allergy Appl Immunol. 1988;86(1):82–91. doi: 10.1159/000234610. [DOI] [PubMed] [Google Scholar]
  11. Ferrante A., Thong Y. H. Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leucocytes from human blood by the Hypaque-Ficoll method. J Immunol Methods. 1980;36(2):109–117. doi: 10.1016/0022-1759(80)90036-8. [DOI] [PubMed] [Google Scholar]
  12. Figari I. S., Mori N. A., Palladino M. A., Jr Regulation of neutrophil migration and superoxide production by recombinant tumor necrosis factors-alpha and -beta: comparison to recombinant interferon-gamma and interleukin-1 alpha. Blood. 1987 Oct;70(4):979–984. [PubMed] [Google Scholar]
  13. Flesch I., Früh A., Ferber E. Functional comparison of bone marrow-derived macrophages obtained by cultivation in serum-free or serum-supplemented medium. Immunobiology. 1986 Oct;173(1):72–81. doi: 10.1016/S0171-2985(86)80091-2. [DOI] [PubMed] [Google Scholar]
  14. Hopkins S. J., Meager A. Cytokines in synovial fluid: II. The presence of tumour necrosis factor and interferon. Clin Exp Immunol. 1988 Jul;73(1):88–92. [PMC free article] [PubMed] [Google Scholar]
  15. Imamura K., Spriggs D., Kufe D. Expression of tumor necrosis factor receptors on human monocytes and internalization of receptor bound ligand. J Immunol. 1987 Nov 1;139(9):2989–2992. [PubMed] [Google Scholar]
  16. Jensen W. A., Rose R. M., Wasserman A. S., Kalb T. H., Anton K., Remold H. G. In vitro activation of the antibacterial activity of human pulmonary macrophages by recombinant gamma interferon. J Infect Dis. 1987 Mar;155(3):574–577. doi: 10.1093/infdis/155.3.574. [DOI] [PubMed] [Google Scholar]
  17. Jungi T. W., Lerch P. G., Brcic M. The effect of recombinant interferon-gamma on human monocyte-derived macrophages. Eur J Immunol. 1987 May;17(5):735–738. doi: 10.1002/eji.1830170526. [DOI] [PubMed] [Google Scholar]
  18. Karhumäki E., Helin H. Regulation of oxidative metabolism by interferon-gamma during human monocyte to macrophage differentiation. Med Biol. 1987;65(5-6):261–266. [PubMed] [Google Scholar]
  19. Kemmerich B., Rossing T. H., Pennington J. E. Comparative oxidative microbicidal activity of human blood monocytes and alveolar macrophages and activation by recombinant gamma interferon. Am Rev Respir Dis. 1987 Aug;136(2):266–270. doi: 10.1164/ajrccm/136.2.266. [DOI] [PubMed] [Google Scholar]
  20. Kharazmi A., Nielsen H., Bendtzen K. Modulation of human neutrophil and monocyte chemotaxis and superoxide responses by recombinant TNF-alpha and GM-CSF. Immunobiology. 1988 Sep;177(4-5):363–370. doi: 10.1016/S0171-2985(88)80004-4. [DOI] [PubMed] [Google Scholar]
  21. Klebanoff S. J., Vadas M. A., Harlan J. M., Sparks L. H., Gamble J. R., Agosti J. M., Waltersdorph A. M. Stimulation of neutrophils by tumor necrosis factor. J Immunol. 1986 Jun 1;136(11):4220–4225. [PubMed] [Google Scholar]
  22. Kowanko I. C., Ferrante A. Stimulation of neutrophil respiratory burst and lysosomal enzyme release by human interferon-gamma. Immunology. 1987 Sep;62(1):149–151. [PMC free article] [PubMed] [Google Scholar]
  23. Kumaratilake L. M., Ferrante A. Purification of human monocytes/macrophages by adherence to cytodex microcarriers. J Immunol Methods. 1988 Sep 13;112(2):183–190. doi: 10.1016/0022-1759(88)90356-0. [DOI] [PubMed] [Google Scholar]
  24. Ming W. J., Bersani L., Mantovani A. Tumor necrosis factor is chemotactic for monocytes and polymorphonuclear leukocytes. J Immunol. 1987 Mar 1;138(5):1469–1474. [PubMed] [Google Scholar]
  25. Murray H. W., Spitalny G. L., Nathan C. F. Activation of mouse peritoneal macrophages in vitro and in vivo by interferon-gamma. J Immunol. 1985 Mar;134(3):1619–1622. [PubMed] [Google Scholar]
  26. Muto S., Minemura H., Yuki H. Rapid effect of interferon-gamma on human monocyte chemiluminescence. Chem Pharm Bull (Tokyo) 1988 May;36(5):1909–1913. doi: 10.1248/cpb.36.1909. [DOI] [PubMed] [Google Scholar]
  27. Nathan C. F., Murray H. W., Wiebe M. E., Rubin B. Y. Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity. J Exp Med. 1983 Sep 1;158(3):670–689. doi: 10.1084/jem.158.3.670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Nathan C. F. Neutrophil activation on biological surfaces. Massive secretion of hydrogen peroxide in response to products of macrophages and lymphocytes. J Clin Invest. 1987 Dec;80(6):1550–1560. doi: 10.1172/JCI113241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Ockenhouse C. F., Schulman S., Shear H. L. Induction of crisis forms in the human malaria parasite Plasmodium falciparum by gamma-interferon-activated, monocyte-derived macrophages. J Immunol. 1984 Sep;133(3):1601–1608. [PubMed] [Google Scholar]
  30. Philip R., Epstein L. B. Tumour necrosis factor as immunomodulator and mediator of monocyte cytotoxicity induced by itself, gamma-interferon and interleukin-1. Nature. 1986 Sep 4;323(6083):86–89. doi: 10.1038/323086a0. [DOI] [PubMed] [Google Scholar]
  31. Sakurai A., Satomi N., Haranaka K. Tumour necrosis factor and the lysosomal enzymes of macrophages or macrophage-like cell line. Cancer Immunol Immunother. 1985;20(1):6–10. doi: 10.1007/BF00199766. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Shalaby M. R., Aggarwal B. B., Rinderknecht E., Svedersky L. P., Finkle B. S., Palladino M. A., Jr Activation of human polymorphonuclear neutrophil functions by interferon-gamma and tumor necrosis factors. J Immunol. 1985 Sep;135(3):2069–2073. [PubMed] [Google Scholar]
  33. Urban J. L., Shepard H. M., Rothstein J. L., Sugarman B. J., Schreiber H. Tumor necrosis factor: a potent effector molecule for tumor cell killing by activated macrophages. Proc Natl Acad Sci U S A. 1986 Jul;83(14):5233–5237. doi: 10.1073/pnas.83.14.5233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Wirth J. J., Kierszenbaum F. Recombinant tumor necrosis factor enhances macrophage destruction of Trypanosoma cruzi in the presence of bacterial endotoxin. J Immunol. 1988 Jul 1;141(1):286–288. [PubMed] [Google Scholar]
  35. Wirth J. J., Kierszenbaum F., Sonnenfeld G., Zlotnik A. Enhancing effects of gamma interferon on phagocytic cell association with and killing of Trypanosoma cruzi. Infect Immun. 1985 Jul;49(1):61–66. doi: 10.1128/iai.49.1.61-66.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES