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. 1991 Mar;83(3):505–509. doi: 10.1111/j.1365-2249.1991.tb05669.x

Human T cell responses to beta-galactosidase.

J D Oxley 1, R H Brookes 1, L S Rayfield 1, P S Shepherd 1
PMCID: PMC1535310  PMID: 1848491

Abstract

The peripheral blood of most normal individuals has been shown to contain T cells that respond to beta-galactosidase (beta-Gal), presumably as a result of natural priming. Three T cell clones (clones 1,2,4) specific for beta-Gal were isolated from peripheral blood mononuclear cells (PBMC) after pretreatment with leucine methyl ester (LeuOMe); a fourth clone from the same individual was isolated from untreated cells. All four clones were CD4+ CD8- alpha beta TcR+ and clone 1 was additionally shown to be cytotoxic. Epstein-Barr virus (EBV) transformed B cell lines were derived from LeuOMe-treated or untreated PBMC and used to study the efficiency of presentation of beta-Gal to one of the clones. The results indicated that B cells transformed after LeuOMe treatment presented beta-Gal at lower concentrations than untreated controls. beta-Gal would therefore appear to be a highly suitable model antigen for studies of immunoregulation in humans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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