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. 1991 Apr;84(1):66–71. doi: 10.1111/j.1365-2249.1991.tb08125.x

The distribution and functional properties of dendritic cells in patients with seronegative arthritis.

A J Stagg 1, B Harding 1, R A Hughes 1, A Keat 1, S C Knight 1
PMCID: PMC1535366  PMID: 1826647

Abstract

Dendritic cells (DC), potent antigen-presenting cells, are known to be increased in numbers in inflammatory lesions in rheumatoid arthritis and juvenile chronic arthritis. In this study, patients with seronegative arthritis were studied; the distribution and functional properties of DC enriched low density cells (LDC) from peripheral blood (PB) and synovial fluid (SF) were compared. The composition of LDC from both sources was similar, comprising approximately 30% DC, 60% monocytes with few T lymphocytes. SF was significantly enriched for LDC compared with paired peripheral blood (P less than 0.0001) or peripheral blood from healthy controls (P less than 0.001). In contrast, patient PB contained fewer LDC (P less than 0.05) overall than healthy controls. LDC from both sources were potent simulators of allogeneic PB T cells in a mixed leucocyte reaction (MLR), but in four out of 10 patients SF LDC were significantly more stimulatory. In autologous MLRs (AMLRs) SF T cells were not stimulated by either LDC population. This anergy of T cells was confined to the joint as patient PB T cells showed an AMLR response to PB LDC which was similar to that seen in cells from healthy controls. PB T cells also responded to SF LDC; in a minority of patients SF LDC caused significantly greater stimulation in AMLR than PB LDC and the possibility is discussed that this may represent presentation of antigen acquired in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austyn J. M. Lymphoid dendritic cells. Immunology. 1987 Oct;62(2):161–170. [PMC free article] [PubMed] [Google Scholar]
  2. Beck P., Burmester G. R., Ledwoch A., Urban C., Kalden J. R. Autologous and allogeneic MLC-reactivity in patients with rheumatoid arthritis. J Clin Lab Immunol. 1981 Jul;6(1):27–33. [PubMed] [Google Scholar]
  3. Crout J. E., McDuffie F. C., Ritts R. E., Jr Induction of peripheral blood lymphocyte transformation by autologous synovial fluid lymphocytes and synovial fluid. Arthritis Rheum. 1976 May-Jun;19(3):523–531. doi: 10.1002/art.1780190303. [DOI] [PubMed] [Google Scholar]
  4. Crow M. K., Kunkel H. G. Human dendritic cells: major stimulators of the autologous and allogeneic mixed leucocyte reactions. Clin Exp Immunol. 1982 Aug;49(2):338–346. [PMC free article] [PubMed] [Google Scholar]
  5. Drexler H. G., Gignac S. M., Brenner M. K., Coustan-Smith E., Janossy G., Hoffbrand A. V. Differential expression of MHC class II antigens in chronic B-cell disorders. Clin Exp Immunol. 1988 Feb;71(2):217–223. [PMC free article] [PubMed] [Google Scholar]
  6. Ford D. K., da Roza D. M., Shah P., Wenman W. M. Cell-mediated immune responses of synovial mononuclear cells in Reiter's syndrome against ureaplasmal and chlamydial antigens. J Rheumatol. 1980 Sep-Oct;7(5):751–755. [PubMed] [Google Scholar]
  7. Gaston J. S., Life P. F., Granfors K., Merilahti-Palo R., Bailey L., Consalvey S., Toivanen A., Bacon P. A. Synovial T lymphocyte recognition of organisms that trigger reactive arthritis. Clin Exp Immunol. 1989 Jun;76(3):348–353. [PMC free article] [PubMed] [Google Scholar]
  8. Granfors K., Jalkanen S., von Essen R., Lahesmaa-Rantala R., Isomäki O., Pekkola-Heino K., Merilahti-Palo R., Saario R., Isomäki H., Toivanen A. Yersinia antigens in synovial-fluid cells from patients with reactive arthritis. N Engl J Med. 1989 Jan 26;320(4):216–221. doi: 10.1056/NEJM198901263200404. [DOI] [PubMed] [Google Scholar]
  9. Greenberg S. J., Olanow C. W., Dawson D. V., Crane B., Roses A. D. Autologous mixed lymphocyte reaction in patients with myasthenia gravis: correlation with disease activity. J Immunol. 1984 Mar;132(3):1229–1236. [PubMed] [Google Scholar]
  10. Harding B., Knight S. C. The distribution of dendritic cells in the synovial fluids of patients with arthritis. Clin Exp Immunol. 1986 Mar;63(3):594–600. [PMC free article] [PubMed] [Google Scholar]
  11. Keat A. Reiter's syndrome and reactive arthritis in perspective. N Engl J Med. 1983 Dec 29;309(26):1606–1615. doi: 10.1056/NEJM198312293092604. [DOI] [PubMed] [Google Scholar]
  12. Knight S. C., Farrant J., Bryant A., Edwards A. J., Burman S., Lever A., Clarke J., Webster A. D. Non-adherent, low-density cells from human peripheral blood contain dendritic cells and monocytes, both with veiled morphology. Immunology. 1986 Apr;57(4):595–603. [PMC free article] [PubMed] [Google Scholar]
  13. Knight S. C., Farrant J., Chan J., Bryant A., Bedford P. A., Bateman C. Induction of autoimmunity with dendritic cells: studies on thyroiditis in mice. Clin Immunol Immunopathol. 1988 Sep;48(3):277–289. doi: 10.1016/0090-1229(88)90021-9. [DOI] [PubMed] [Google Scholar]
  14. Knight S. C., Fryer P., Griffiths S., Harding B., Dixey J., Mansell B. Class II antigens on dendritic cells from the synovial fluids of patients with inflammatory arthritis. Clin Exp Immunol. 1989 Oct;78(1):19–25. [PMC free article] [PubMed] [Google Scholar]
  15. Knight S. C., Harding B., Burman S., Mertin J. Cell number requirements for lymphocyte stimulation in vitro: changes during the course of multiple sclerosis and the effects of immunosuppression. Clin Exp Immunol. 1981 Oct;46(1):61–69. [PMC free article] [PubMed] [Google Scholar]
  16. Knight S. C. The autologous mixed lymphocyte reaction and dendritic cells. Br J Rheumatol. 1987 Apr;26(2):85–88. doi: 10.1093/rheumatology/26.2.85. [DOI] [PubMed] [Google Scholar]
  17. Macatonia S. E., Edwards A. J., Knight S. C. Dendritic cells and the initiation of contact sensitivity to fluorescein isothiocyanate. Immunology. 1986 Dec;59(4):509–514. [PMC free article] [PubMed] [Google Scholar]
  18. Nussenzweig M. C., Steinman R. M. Contribution of dendritic cells to stimulation of the murine syngeneic mixed leukocyte reaction. J Exp Med. 1980 May 1;151(5):1196–1212. doi: 10.1084/jem.151.5.1196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Opelz G., Kiuchi M., Takasugi M., Terasaki P. I. Autologous stimulation of human lymphocyte subpopulation. J Exp Med. 1975 Nov 1;142(5):1327–1333. doi: 10.1084/jem.142.5.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pope R. M., McChesney L., Talal N., Fischbach M. Characterization of the defective autologous mixed lymphocyte response in rheumatoid arthritis. Arthritis Rheum. 1984 Nov;27(11):1234–1244. doi: 10.1002/art.1780271105. [DOI] [PubMed] [Google Scholar]
  21. Poulter L. W., Campbell D. A., Munro C., Janossy G. Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies. Scand J Immunol. 1986 Sep;24(3):351–357. doi: 10.1111/j.1365-3083.1986.tb02104.x. [DOI] [PubMed] [Google Scholar]
  22. Poulter L. W., Duke O., Hobbs S., Janossy G., Panayi G. Histochemical discrimination of HLA-DR positive cell populations in the normal and arthritic synovial lining. Clin Exp Immunol. 1982 May;48(2):381–388. [PMC free article] [PubMed] [Google Scholar]
  23. Ransohoff R. M., Dustoor M. M. Impaired autologous mixed lymphocyte reaction with normal concanavalin A-induced suppression in adult polymyositis/dermatomyositis. Clin Exp Immunol. 1983 Jul;53(1):67–75. [PMC free article] [PubMed] [Google Scholar]
  24. Sauvezie B., Miyasaka N., Charron D., Kielich C., Loeb J., Daniels T. E., Talal N. An increase in peripheral blood Ia-positive T cells in Sjögren's syndrome correlates with a decrease in the autologous mixed lymphocyte response. Clin Exp Immunol. 1982 Jul;49(1):50–58. [PMC free article] [PubMed] [Google Scholar]
  25. Saxon A., Feldhaus J., Robins R. A. Single step separation of human T and B cells using AET treated srbc rosettes. J Immunol Methods. 1976;12(3-4):285–288. doi: 10.1016/0022-1759(76)90050-8. [DOI] [PubMed] [Google Scholar]
  26. Silver R. M., Redelman D., Zvaifler N. J. Studies of rheumatoid synovial fluid lymphocytes. II. A comparison of their behavior with blood mononuclear cells in the autologous mixed lymphocyte reaction and response to TCGF. Clin Immunol Immunopathol. 1983 Apr;27(1):15–27. doi: 10.1016/0090-1229(83)90052-1. [DOI] [PubMed] [Google Scholar]
  27. Smith J. B., DeHoratius R. J. Deficient autologous mixed lymphocyte reactions correlate with disease activity in systemic lupus erythematosus and rheumatoid arthritis. Clin Exp Immunol. 1982 Apr;48(1):155–162. [PMC free article] [PubMed] [Google Scholar]
  28. Van Voorhis W. C., Hair L. S., Steinman R. M., Kaplan G. Human dendritic cells. Enrichment and characterization from peripheral blood. J Exp Med. 1982 Apr 1;155(4):1172–1187. doi: 10.1084/jem.155.4.1172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Waalen K., Thoen J., Førre O., Hovig T., Teigland J., Natvig J. B. Rheumatoid synovial dendritic cells as stimulators in allogeneic and autologous mixed leukocyte reactions--comparison with autologous monocytes as stimulator cells. Scand J Immunol. 1986 Feb;23(2):233–241. doi: 10.1111/j.1365-3083.1986.tb01962.x. [DOI] [PubMed] [Google Scholar]
  30. Zvaifler N. J., Steinman R. M., Kaplan G., Lau L. L., Rivelis M. Identification of immunostimulatory dendritic cells in the synovial effusions of patients with rheumatoid arthritis. J Clin Invest. 1985 Aug;76(2):789–800. doi: 10.1172/JCI112036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. de Vere Tyndall A., Knight S. C., Edwards A. J., Clarke J. B. Veiled (dendritic) cells in synovial fluid. Lancet. 1983 Feb 26;1(8322):472–473. doi: 10.1016/s0140-6736(83)91468-x. [DOI] [PubMed] [Google Scholar]

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