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. 1991 Apr;84(1):72–77. doi: 10.1111/j.1365-2249.1991.tb08126.x

Peripheral blood and synovial fluid T cells differ in their response to alloantigens and recall antigens presented by dendritic cells.

A J Stagg 1, B Harding 1, R A Hughes 1, A Keat 1, S C Knight 1
PMCID: PMC1535375  PMID: 1826648

Abstract

Properties of T cells from inflammatory lesions were analysed by comparing the response of peripheral blood (PB) and synovial fluid (SF) T cells from 19 patients with a range of arthropathies to enriched allogeneic dendritic cells (DC) in a primary mixed leucocyte reaction (MLR). In 17 patients the proliferative response of SF T cells was significantly (P less than 0.05) less than that of PB lymphocytes. The reduced response of SF T cells was observed in all disease categories studied and could not be attributed to differences in cell number requirements or response kinetics. Addition of recombinant interleukin-2 enhanced the response of SF T cells in a dose-dependent manner. Cell mixing experiments suggested that active suppression was not the underlying mechanism of the poor MLR response of SF T cells. In contrast to the MLR response. SF T cells were able to mount vigorous proliferative responses to recall antigen presented by autologous antigen-presenting cells. The possibility is discussed that T cells compartmentalized at inflammatory lesions are a unique population with a diminished ability to interact with DC and respond to primary stimuli but an ability to respond to secondary antigenic challenge.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akbar A. N., Terry L., Timms A., Beverley P. C., Janossy G. Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J Immunol. 1988 Apr 1;140(7):2171–2178. [PubMed] [Google Scholar]
  2. Austyn J. M. Lymphoid dendritic cells. Immunology. 1987 Oct;62(2):161–170. [PMC free article] [PubMed] [Google Scholar]
  3. Austyn J. M., Steinman R. M., Weinstein D. E., Granelli-Piperno A., Palladino M. A. Dendritic cells initiate a two-stage mechanism for T lymphocyte proliferation. J Exp Med. 1983 Apr 1;157(4):1101–1115. doi: 10.1084/jem.157.4.1101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beck P., Burmester G. R., Ledwoch A., Urban C., Kalden J. R. Autologous and allogeneic MLC-reactivity in patients with rheumatoid arthritis. J Clin Lab Immunol. 1981 Jul;6(1):27–33. [PubMed] [Google Scholar]
  5. Bedford P. A., Knight S. C. The effect of retinoids on dendritic cell function. Clin Exp Immunol. 1989 Mar;75(3):481–486. [PMC free article] [PubMed] [Google Scholar]
  6. Burmester G. R., Kalden J. R., Peter H. H., Schedel I., Beck P., Wittenborg A. Immunological and functional characteristics of peripheral blood and synovial fluid lymphocytes from patients with rheumatoid arthritis. Scand J Immunol. 1978;7(5):405–417. doi: 10.1111/j.1365-3083.1978.tb00471.x. [DOI] [PubMed] [Google Scholar]
  7. Combe B., Pope R. M., Fischbach M., Darnell B., Baron S., Talal N. Interleukin-2 in rheumatoid arthritis: production of and response to interleukin-2 in rheumatoid synovial fluid, synovial tissue and peripheral blood. Clin Exp Immunol. 1985 Mar;59(3):520–528. [PMC free article] [PubMed] [Google Scholar]
  8. Duke O., Panayi G. S., Janossy G., Poulter L. W., Tidman N. Analysis of T cell subsets in the peripheral blood and synovial fluid of patients with rheumatoid arthritis by means of monoclonal antibodies. Ann Rheum Dis. 1983 Aug;42(4):357–361. doi: 10.1136/ard.42.4.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ford D. K., da Roza D. M., Shah P. Cell-mediated immune responses of synovial mononuclear cells to sexually transmitted, enteric and mumps antigens in patients with Reiter's syndrome, rheumatoid arthritis and ankylosing spondylitis. J Rheumatol. 1981 Mar-Apr;8(2):220–232. [PubMed] [Google Scholar]
  10. Fox R. I., Fong S., Sabharwal N., Carstens S. A., Kung P. C., Vaughan J. H. Synovial fluid lymphocytes differ from peripheral blood lymphocytes in patients with rheumatoid arthritis. J Immunol. 1982 Jan;128(1):351–354. [PubMed] [Google Scholar]
  11. Green J., Jotte R. Interactions between T helper cells and dendritic cells during the rat mixed lymphocyte reaction. J Exp Med. 1985 Nov 1;162(5):1546–1560. doi: 10.1084/jem.162.5.1546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Inaba K., Steinman R. M. Accessory cell-T lymphocyte interactions. Antigen-dependent and -independent clustering. J Exp Med. 1986 Feb 1;163(2):247–261. doi: 10.1084/jem.163.2.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Keat A., Thomas B. J., Taylor-Robinson D. Chlamydial infection in the aetiology of arthritis. Br Med Bull. 1983 Apr;39(2):168–174. doi: 10.1093/oxfordjournals.bmb.a071811. [DOI] [PubMed] [Google Scholar]
  14. Keystone E. C., Minden M., Klock R., Poplonski L., Zalcberg J., Takadera T., Mak T. W. Structure of T cell antigen receptor beta chain in synovial fluid cells from patients with rheumatoid arthritis. Arthritis Rheum. 1988 Dec;31(12):1555–1557. doi: 10.1002/art.1780311213. [DOI] [PubMed] [Google Scholar]
  15. Kingsley G., Pitzalis C., Kyriazis N., Panayi G. S. Abnormal helper-inducer/suppressor-inducer T-cell subset distribution and T-cell activation status are common to all types of chronic synovitis. Scand J Immunol. 1988 Aug;28(2):225–232. doi: 10.1111/j.1365-3083.1988.tb02435.x. [DOI] [PubMed] [Google Scholar]
  16. Knight S. C., Farrant J., Bryant A., Edwards A. J., Burman S., Lever A., Clarke J., Webster A. D. Non-adherent, low-density cells from human peripheral blood contain dendritic cells and monocytes, both with veiled morphology. Immunology. 1986 Apr;57(4):595–603. [PMC free article] [PubMed] [Google Scholar]
  17. Knight S. C., Harding B., Burman S., Mertin J. Cell number requirements for lymphocyte stimulation in vitro: changes during the course of multiple sclerosis and the effects of immunosuppression. Clin Exp Immunol. 1981 Oct;46(1):61–69. [PMC free article] [PubMed] [Google Scholar]
  18. Koide S. L., Inaba K., Steinman R. M. Interleukin 1 enhances T-dependent immune responses by amplifying the function of dendritic cells. J Exp Med. 1987 Feb 1;165(2):515–530. doi: 10.1084/jem.165.2.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Macatonia S. E., Knight S. C. Dendritic cells and T cells transfer sensitization for delayed-type hypersensitivity after skin painting with contact sensitizer. Immunology. 1989 Jan;66(1):96–99. [PMC free article] [PubMed] [Google Scholar]
  20. Macatonia S. E., Taylor P. M., Knight S. C., Askonas B. A. Primary stimulation by dendritic cells induces antiviral proliferative and cytotoxic T cell responses in vitro. J Exp Med. 1989 Apr 1;169(4):1255–1264. doi: 10.1084/jem.169.4.1255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Miossec P., Kashiwado T., Ziff M. Inhibitor of interleukin-2 in rheumatoid synovial fluid. Arthritis Rheum. 1987 Feb;30(2):121–129. doi: 10.1002/art.1780300201. [DOI] [PubMed] [Google Scholar]
  22. Morimoto C., Letvin N. L., Boyd A. W., Hagan M., Brown H. M., Kornacki M. M., Schlossman S. F. The isolation and characterization of the human helper inducer T cell subset. J Immunol. 1985 Jun;134(6):3762–3769. [PubMed] [Google Scholar]
  23. Sanders M. E., Makgoba M. W., Shaw S. Human naive and memory T cells: reinterpretation of helper-inducer and suppressor-inducer subsets. Immunol Today. 1988 Jul-Aug;9(7-8):195–199. doi: 10.1016/0167-5699(88)91212-1. [DOI] [PubMed] [Google Scholar]
  24. Santiago-Schwarz F., Bakke A. C., Woodward J. G., O'Brien R. L., Horwitz D. A. Further characterization of low density mononuclear cells: FACS-assisted analysis of human MLR stimulators. J Immunol. 1985 Feb;134(2):779–785. [PubMed] [Google Scholar]
  25. Saxon A., Feldhaus J., Robins R. A. Single step separation of human T and B cells using AET treated srbc rosettes. J Immunol Methods. 1976;12(3-4):285–288. doi: 10.1016/0022-1759(76)90050-8. [DOI] [PubMed] [Google Scholar]
  26. Stagg A. J., Harding B., Hughes R. A., Keat A., Knight S. C. The distribution and functional properties of dendritic cells in patients with seronegative arthritis. Clin Exp Immunol. 1991 Apr;84(1):66–71. doi: 10.1111/j.1365-2249.1991.tb08125.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Synovial fluid T cells and 65 kD heat-shock protein. Lancet. 1988 Oct 8;2(8615):856–857. [PubMed] [Google Scholar]
  28. Van Voorhis W. C., Hair L. S., Steinman R. M., Kaplan G. Human dendritic cells. Enrichment and characterization from peripheral blood. J Exp Med. 1982 Apr 1;155(4):1172–1187. doi: 10.1084/jem.155.4.1172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. de Vere Tyndall A., Knight S. C., Edwards A. J., Clarke J. B. Veiled (dendritic) cells in synovial fluid. Lancet. 1983 Feb 26;1(8322):472–473. doi: 10.1016/s0140-6736(83)91468-x. [DOI] [PubMed] [Google Scholar]

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