Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1991 May;84(2):238–244. doi: 10.1111/j.1365-2249.1991.tb08155.x

Characterization of anti-endothelial cell antibodies in systemic lupus erythematosus (SLE).

J M van der Zee 1, C E Siegert 1, T A de Vreede 1, M R Daha 1, F C Breedveld 1
PMCID: PMC1535404  PMID: 2025951

Abstract

IgG anti-endothelial antibodies (AEA), as measured by ELISA or immunoblotting technique could be detected in serum samples of 56 out of 64 patients with SLE (88%) and mainly occurred in monomeric form. AEA were not cell specific, because the binding reactivity was absorbed partially by both fibroblasts and peripheral blood mononuclear cells. No correlation was found between the presence of AEA and anti-nuclear antibodies. Immunoblotting revealed reactivity of AEA against endothelial antigens ranging in size from 15 to 200 kD. AEA titres were significantly higher in patients with joint or skin abnormalities, compared with patients without these abnormalities. A significant correlation was found between nephritis in SLE and the presence of AEA reactivity against endothelial membrane antigens of 38, 41 and 150 kD. These data show that the pattern of AEA reactivity in serum of SLE patients is heterogeneous, and suggest that AEA against a limited number of antigens may be involved in the pathogenesis of nephritis in SLE.

Full text

PDF
238

Images in this article

240Fig. 3
on p.240
240Fig. 4
on p.240

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aarden L. A., de Groot E. R., Feltkamp T. E. Immunology of DNA. III. Crithidia luciliae, a simple substrate for the determination of anti-dsDNA with the immunofluorescence technique. Ann N Y Acad Sci. 1975 Jun 30;254:505–515. doi: 10.1111/j.1749-6632.1975.tb29197.x. [DOI] [PubMed] [Google Scholar]
  2. Brasile L., Kremer J. M., Clarke J. L., Cerilli J. Identification of an autoantibody to vascular endothelial cell-specific antigens in patients with systemic vasculitis. Am J Med. 1989 Jul;87(1):74–80. doi: 10.1016/s0002-9343(89)80486-3. [DOI] [PubMed] [Google Scholar]
  3. Breedveld F. C., Heurkens A. H., Lafeber G. J., van Hinsbergh V. W., Cats A. Immune complexes in sera from patients with rheumatoid vasculitis induce polymorphonuclear cell-mediated injury to endothelial cells. Clin Immunol Immunopathol. 1988 Aug;48(2):202–213. doi: 10.1016/0090-1229(88)90084-0. [DOI] [PubMed] [Google Scholar]
  4. Burns E. R., Zucker-Franklin D. Pathologic effects of plasma from patients with thrombotic thrombocytopenic purpura on platelets and cultured vascular endothelial cells. Blood. 1982 Oct;60(4):1030–1037. [PubMed] [Google Scholar]
  5. Fattorossi A., Aurbach G. D., Sakaguchi K., Cama A., Marx S. J., Streeten E. A., Fitzpatrick L. A., Brandi M. L. Anti-endothelial cell antibodies: detection and characterization in sera from patients with autoimmune hypoparathyroidism. Proc Natl Acad Sci U S A. 1988 Jun;85(11):4015–4019. doi: 10.1073/pnas.85.11.4015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ferraro G., Meroni P. L., Tincani A., Sinico A., Barcellini W., Radice A., Gregorini G., Froldi M., Borghi M. O., Balestrieri G. Anti-endothelial cell antibodies in patients with Wegener's granulomatosis and micropolyarteritis. Clin Exp Immunol. 1990 Jan;79(1):47–53. doi: 10.1111/j.1365-2249.1990.tb05125.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hashemi S., Smith C. D., Izaguirre C. A. Anti-endothelial cell antibodies: detection and characterization using a cellular enzyme-linked immunosorbent assay. J Lab Clin Med. 1987 Apr;109(4):434–440. [PubMed] [Google Scholar]
  8. Heurkens A. H., Hiemstra P. S., Lafeber G. J., Daha M. R., Breedveld F. C. Anti-endothelial cell antibodies in patients with rheumatoid arthritis complicated by vasculitis. Clin Exp Immunol. 1989 Oct;78(1):7–12. [PMC free article] [PubMed] [Google Scholar]
  9. Jaffe E. A., Nachman R. L., Becker C. G., Minick C. R. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 1973 Nov;52(11):2745–2756. doi: 10.1172/JCI107470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kurata N., Tan E. M. Identification of antibodies to nuclear acidic antigens by counterimmunoelectrophoresis. Arthritis Rheum. 1976 May-Jun;19(3):574–580. doi: 10.1002/art.1780190309. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Le Roux G., Wautier M. P., Guillevin L., Wautier J. L. IgG binding to endothelial cells in systemic lupus erythematosus. Thromb Haemost. 1986 Oct 21;56(2):144–146. [PubMed] [Google Scholar]
  13. Leung D. Y., Collins T., Lapierre L. A., Geha R. S., Pober J. S. Immunoglobulin M antibodies present in the acute phase of Kawasaki syndrome lyse cultured vascular endothelial cells stimulated by gamma interferon. J Clin Invest. 1986 May;77(5):1428–1435. doi: 10.1172/JCI112454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Leung D. Y., Moake J. L., Havens P. L., Kim M., Pober J. S. Lytic anti-endothelial cell antibodies in haemolytic-uraemic syndrome. Lancet. 1988 Jul 23;2(8604):183–186. doi: 10.1016/s0140-6736(88)92287-8. [DOI] [PubMed] [Google Scholar]
  15. Maciag T., Cerundolo J., Ilsley S., Kelley P. R., Forand R. An endothelial cell growth factor from bovine hypothalamus: identification and partial characterization. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5674–5678. doi: 10.1073/pnas.76.11.5674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Miltenburg A. M., Meijer-Paape M. E., Daha M. R., Paul L. C. Endothelial cell lysis induced by lymphokine-activated human peripheral blood mononuclear cells. Eur J Immunol. 1987 Sep;17(9):1383–1386. doi: 10.1002/eji.1830170926. [DOI] [PubMed] [Google Scholar]
  17. Morrow W. J., Isenberg D. A., Todd-Pokropek A., Parry H. F., Snaith M. L. Useful laboratory measurements in the management of systemic lupus erythematosus. Q J Med. 1982 Spring;51(202):125–138. [PubMed] [Google Scholar]
  18. Paul L. C., Carpenter C. B. Antibodies against renal endothelial alloantigens. Transplant Proc. 1980 Sep;12(3 Suppl 1):43–48. [PubMed] [Google Scholar]
  19. Rosenbaum J., Pottinger B. E., Woo P., Black C. M., Loizou S., Byron M. A., Pearson J. D. Measurement and characterisation of circulating anti-endothelial cell IgG in connective tissue diseases. Clin Exp Immunol. 1988 Jun;72(3):450–456. [PMC free article] [PubMed] [Google Scholar]
  20. Searles R. P., Messner R. P., Bankhurst A. D. Cross--reactivity of antilymphocyte and antinuclear antibodies in systemic lupus erythematosus. Clin Immunol Immunopathol. 1979 Nov;14(3):292–299. doi: 10.1016/0090-1229(79)90155-7. [DOI] [PubMed] [Google Scholar]
  21. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]
  22. Thornton S. C., Mueller S. N., Levine E. M. Human endothelial cells: use of heparin in cloning and long-term serial cultivation. Science. 1983 Nov 11;222(4624):623–625. doi: 10.1126/science.6635659. [DOI] [PubMed] [Google Scholar]
  23. Urowitz M. B., Gladman D. D., Tozman E. C., Goldsmith C. H. The lupus activity criteria count (LACC). J Rheumatol. 1984 Dec;11(6):783–787. [PubMed] [Google Scholar]
  24. Valentijn R. M., van Overhagen H., Hazevoet H. M., Hermans J., Cats A., Daha M. R., van ES L. A. The value of complement and immune complex determinations in monitoring disease activity in patients with systemic lupus erythematosus. Arthritis Rheum. 1985 Aug;28(8):904–913. doi: 10.1002/art.1780280810. [DOI] [PubMed] [Google Scholar]
  25. Vismara A., Meroni P. L., Tincani A., Harris E. N., Barcellini W., Brucato A., Khamashta M., Hughes G. R., Zanussi C., Balestrieri G. Relationship between anti-cardiolipin and anti-endothelial cell antibodies in systemic lupus erythematosus. Clin Exp Immunol. 1988 Nov;74(2):247–253. [PMC free article] [PubMed] [Google Scholar]
  26. Westgeest A. A., Bons J. C., Van den Brink H. G., Aarden L. A., Smeenk R. J. An improved incubation apparatus for Western blots used for the detection of antinuclear antibodies. J Immunol Methods. 1986 Dec 24;95(2):283–288. doi: 10.1016/0022-1759(86)90417-5. [DOI] [PubMed] [Google Scholar]
  27. Wood N. L., Schook L. B., Studer E. J., Mohanakumar T. Biochemical characterization of human vascular endothelial cell-monocyte antigens defined by monoclonal antibodies. Transplantation. 1988 Apr;45(4):787–792. doi: 10.1097/00007890-198804000-00024. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES