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. 1991 Jan;83(1):137–142. doi: 10.1111/j.1365-2249.1991.tb05603.x

A new murine model of autoimmune orchitis induced by immunization with viable syngeneic testicular germ cells alone. I. Immunological and histological studies.

M Itoh 1, C Hiramine 1, K Hojo 1
PMCID: PMC1535462  PMID: 1988220

Abstract

Experimental autoimmune orchitis (EAO) was produced in C3H/He mice with as high as 100% incidence by two or three s.c. injections of 1 x 10(7) viable syngeneic testicular germ cells (TC) without resorting to adjuvants, Bordetella pertussis vaccine, or other immunological manipulations. On day 40 after the first injection of TC, the lesions induced were characterized by interstitial infiltration of inflammatory cells and severe hypospermatogenesis in the testis with resulting whole organ atrophy and, in contrast, by a complete lack of epididymitis. Immunological studies revealed that this form of immunization caused both delayed-type hypersensitivity and humoral antibody responses to syngeneic TC. We compared the susceptibilities to the induction of this type of EAO among six different strains of inbred mice comprising A/J, AKR, BALB/c, C3H/He, C57BL/6 and DBA/2 mice. All strains except for DBA/2 mice developed lesions of EAO to a greater or lesser extent, and severe disease was induced with high frequency in two strains, C3H/He and A/J. As this murine model of EAO can be induced without the use of Freund's complete adjuvant and B. pertussis vaccine, it is simply 'autoimmune' in nature and may provide new ways for further investigation into the immunological mechanisms which regulate deleterious autoimmune reactions to germ cell antigens leading to the male infertility.

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Selected References

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  1. Adekunle A. O., Hickey W. F., Smith S. M., Tung K. S., Teuscher C. Experimental allergic orchitis in mice: IV. Preliminary characterization of the major murine testis specific aspermatogenic autoantigen(s). J Reprod Immunol. 1987 Sep;12(1):49–62. doi: 10.1016/0165-0378(87)90080-5. [DOI] [PubMed] [Google Scholar]
  2. Bernard C. C., Mitchell G. F., Leydon J., Bargerbos A. Experimental autoimmune orchitis in T-cell-deficient mice. Int Arch Allergy Appl Immunol. 1978;56(3):256–263. doi: 10.1159/000232029. [DOI] [PubMed] [Google Scholar]
  3. Hargis B. J., Malkiel S., Berkelhammer J. Immunologically induced aspermatogenesis in the white mouse. J Immunol. 1968 Aug;101(2):374–376. [PubMed] [Google Scholar]
  4. Hiramine C., Hojo K. Adoptive transfer of suppression of experimental allergic orchitis with lymphoid cells from antigen-pretreated guinea pigs. Clin Immunol Immunopathol. 1984 Feb;30(2):202–213. doi: 10.1016/0090-1229(84)90055-2. [DOI] [PubMed] [Google Scholar]
  5. Hiramine C., Hojo K. Requirement of B lymphocytes in local adoptive transfer of experimental allergic orchitis (EAO) by lymph node cells. Am J Reprod Immunol. 1984 Oct-Nov;6(3):85–91. doi: 10.1111/j.1600-0897.1984.tb00120.x. [DOI] [PubMed] [Google Scholar]
  6. Hojo K., Hiramine C. In vivo effects of cyclosporin A: abrogation of the induction of experimental allergic orchitis and sparing of the generation of suppressor cells. Int Arch Allergy Appl Immunol. 1985;78(1):63–70. doi: 10.1159/000233864. [DOI] [PubMed] [Google Scholar]
  7. Hojo K., Hiramine C. Suppression of experimental allergic orchitis and cellular immune response in the guinea pig by pretreatment with testis antigen in incomplete Freund's adjuvant. Int Arch Allergy Appl Immunol. 1982;69(1):40–49. doi: 10.1159/000233144. [DOI] [PubMed] [Google Scholar]
  8. Ichinohasama R., Hirokawa K., Hatakeyama S. Spermatogenic disturbance induced in mice by combined local injection of monoclonal antibodies to Sertoli cell and to basal lamina of seminiferous tubule. Am J Reprod Immunol Microbiol. 1986 Apr;10(4):158–165. doi: 10.1111/j.1600-0897.1986.tb00017.x. [DOI] [PubMed] [Google Scholar]
  9. Itoh M., Hiramine C., Koseto M., Hojo K. Enzyme-linked immunosorbent assay (ELISA) for detecting antisperm antibodies in mice with testicular autoimmunity. Am J Reprod Immunol. 1989 Sep;21(1):9–15. doi: 10.1111/j.1600-0897.1989.tb00991.x. [DOI] [PubMed] [Google Scholar]
  10. Kojima A., Prehn R. T. Genetic susceptibility to post-thymectomy autoimmune diseases in mice. Immunogenetics. 1981;14(1-2):15–27. doi: 10.1007/BF00344296. [DOI] [PubMed] [Google Scholar]
  11. Pelletier R. M., Nemirovsky M. S., Calvert R., Hugon J. S. Effects of immunization with Freund's complete adjuvant and isologous spermatozoa on the seminiferous epithelium and blood-testis barrier in guinea pigs. Anat Rec. 1981 Feb;199(2):197–211. doi: 10.1002/ar.1091990205. [DOI] [PubMed] [Google Scholar]
  12. Sakamoto Y., Himeno K., Sanui H., Yoshida S., Nomoto K. Experimental allergic orchitis in mice. I. A new model induced by immunization without adjuvants. Clin Immunol Immunopathol. 1985 Dec;37(3):360–368. doi: 10.1016/0090-1229(85)90105-9. [DOI] [PubMed] [Google Scholar]
  13. Sato K., Hirokawa K., Hatakeyama S. Experimental allergic orchitis in mice. Histopathological and Immunological studies. Virchows Arch A Pathol Anat Histol. 1981;392(2):147–158. doi: 10.1007/BF00430817. [DOI] [PubMed] [Google Scholar]
  14. Sewell W. A., de Moerloose P. A., McKimm-Breschkin J. L., Vadas M. A. Pertussigen enhances antigen-driven interferon-gamma production by sensitized lymphoid cells. Cell Immunol. 1986 Feb;97(2):238–247. doi: 10.1016/0008-8749(86)90394-1. [DOI] [PubMed] [Google Scholar]
  15. Taguchi O., Nishizuka Y. Experimental autoimmune orchitis after neonatal thymectomy in the mouse. Clin Exp Immunol. 1981 Nov;46(2):425–434. [PMC free article] [PubMed] [Google Scholar]
  16. Teuscher C., Smith S. M., Goldberg E. H., Shearer G. M., Tung K. S. Experimental allergic orchitis in mice. I. Genetic control of susceptibility and resistance to induction of autoimmune orchitis. Immunogenetics. 1985;22(4):323–333. doi: 10.1007/BF00430916. [DOI] [PubMed] [Google Scholar]
  17. Tung K. S., Leong C., McCarty T. Pathogenesis of experimental allergic orchitis. III. T lymphocyte requirement in local adoptive transfer by peritoneal exudate cells. J Immunol. 1977 May;118(5):1774–1779. [PubMed] [Google Scholar]

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