Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1990 Dec;82(3):522–526. doi: 10.1111/j.1365-2249.1990.tb05483.x

Temporary breakdown of immunological tolerance to dsDNA and nucleohistone antigens in rabbits infected with rinderpest virus.

K Imaoka 1, Y Kanai 1, Y Yoshikawa 1, K Yamanouchi 1
PMCID: PMC1535499  PMID: 2265490

Abstract

The rabbit-passaged L strain of rinderpest virus (RV) causes the transient induction of anti-nuclear antibodies (ANA) in rabbits. It has been shown by an indirect immunofluorescence test that the target antigens of these ANA are DNA and/or DNA-histone complexes (nucleohistone). Here detailed examinations of the target antigens were carried out by ELISA, and it was revealed that rabbit sera contained three types of antibodies: antibodies reacting equally with both dsDNA and ssDNA; those reacting with ssDNA alone; and those reacting with nucleohistone. Epitopes recognized by the third type consisted of complexes of dsDNA and H2A + H2B or of dsDNA and H2B. All types of antibodies were antigen specific. Since the diversity of ANA among experimental rabbits was large, it was suggested that genetic background is important in the induction of anti-dsDNA antibodies in this system. Moreover, early induction of antibodies to nucleohistone and the rapid disappearance of ANA suggest that B cell proliferation/maturation for continuous production of ANA requires factors other than RV infection. This system may help elucidate the mechanisms of ANA induction and the development of autoimmune diseases.

Full text

PDF
522

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arana R., Seligmann M. Antibodies to native and denatured deoxyribonucleic acid in systemic lupus erythematosus. J Clin Invest. 1967 Nov;46(11):1867–1882. doi: 10.1172/JCI105677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Faiferman I., Koffler D. Reaction of antipolynucleotide antibody from systemic lupus erythematosus patient serum with double-stranded DNA complexed to protein. Arthritis Rheum. 1987 Jul;30(7):814–818. doi: 10.1002/art.1780300712. [DOI] [PubMed] [Google Scholar]
  3. Flaegstad T., Fredriksen K., Dahl B., Traavik T., Rekvig O. P. Inoculation with BK virus may break immunological tolerance to histone and DNA antigens. Proc Natl Acad Sci U S A. 1988 Nov;85(21):8171–8175. doi: 10.1073/pnas.85.21.8171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fukuda A., Yamanouchi K. Autoimmunity induced in rabbits by rinderpest virus. Infect Immun. 1976 May;13(5):1449–1453. doi: 10.1128/iai.13.5.1449-1453.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Imaoka K., Yoshikawa Y., Kanai Y., Yamanouchi K. Characterization of antinuclear antibodies induced in rabbits by rinderpest virus infection. Arch Virol. 1988;102(1-2):111–118. doi: 10.1007/BF01315567. [DOI] [PubMed] [Google Scholar]
  6. Kanai Y., Akatsuka T., Kubota T., Goto S., Stollar B. D. MRL/Mp-lpr/lpr mouse derived monoclonal antibodies that recognise determinants shared by poly (ADP-ribose), single stranded DNA and left handed Z-DNA. Clin Exp Immunol. 1985 Jan;59(1):139–145. [PMC free article] [PubMed] [Google Scholar]
  7. Kanai Y., Fujiwara M. Naturally occurring antibodies to poly(ADP-ribose) in autoimmune MRL/Mp-lpr/lpr mice. Clin Exp Immunol. 1985 Jan;59(1):132–138. [PMC free article] [PubMed] [Google Scholar]
  8. Kanai Y., Yamauchi S., Hashiba-Kanai Y. Lupus erythematosus cell formation by a monoclonal antibody derived from an autoimmune MRL/Mp-lpr/lpr mouse. Immunol Lett. 1989 Jan 15;20(1):9–13. doi: 10.1016/0165-2478(89)90061-8. [DOI] [PubMed] [Google Scholar]
  9. Kanai Y., Yaoita Y., Imaoka K., Honjo T. A unique monoclonal antibody derived from a lupus-prone mouse with multiple bindings to autoantigens associated with rheumatic disease. Immunol Lett. 1988 Sep;19(1):15–20. doi: 10.1016/0165-2478(88)90113-7. [DOI] [PubMed] [Google Scholar]
  10. NAKAMURA J., MIYAMOTO T. Avianization of lapinized rinderpest virus. Am J Vet Res. 1953 Apr;14(51):307–317. [PubMed] [Google Scholar]
  11. Panyim S., Chalkley R. High resolution acrylamide gel electrophoresis of histones. Arch Biochem Biophys. 1969 Mar;130(1):337–346. doi: 10.1016/0003-9861(69)90042-3. [DOI] [PubMed] [Google Scholar]
  12. Stollar B. D., Papalian M. Secondary structure in denatured DNA is responsible for its reaction with antinative DNA antibodies of systemic lupus erythematosus sera. J Clin Invest. 1980 Aug;66(2):210–219. doi: 10.1172/JCI109846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tan E. M. Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine. Adv Immunol. 1982;33:167–240. doi: 10.1016/s0065-2776(08)60836-6. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES