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. 1983 Jul;53(1):165–174.

Circulating immune complexes in patients with Candida albicans infections.

G Burges, H P Holley, G Virella
PMCID: PMC1535523  PMID: 6872325

Abstract

The existence of circulating immune complexes in patients with candidiasis has been suggested but not confirmed. In this study we have used four immune complex screening techniques (direct nephelometry, Clq binding assay, PEG-IgG assay and PEG-C4 assay) and an immune complex score compiled from results of the individual tests to establish that elevated levels of immune complexes do exist in a series of 11 patients with systemic candidiasis as compared with a group of 18 normal controls. Isolation and characterization of high molecular weight fractions from the sera of five patients with candidiasis and further purification by affinity chromatography on Sepharose 4B-protein A substantiated the presence of anti-Candida antibody and/or Candida antigen in at least three of them. The presence of complement components and IgG in these fractions further supported the existence of immune complexes containing microbial antigen and antibody.

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Selected References

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  1. Bullock W. E., Artz R. P., Bhathena D., Tung K. S. Histoplasmosis. Association with circulating immune complexes, eosinophilia, and mesangiopathic glomerulonephritis. Arch Intern Med. 1979 Jun;139(6):700–702. doi: 10.1001/archinte.139.6.700. [DOI] [PubMed] [Google Scholar]
  2. Digeon M., Laver M., Riza J., Bach J. F. Detection of circulating immune complexes in human sera by simplified assays with polyethylene glycol. J Immunol Methods. 1977;16(2):165–183. doi: 10.1016/0022-1759(77)90051-5. [DOI] [PubMed] [Google Scholar]
  3. Estes G. B., Muñoz M., Burdash N. M., Virella G. A quantitative immunofluorescence test for the detection of anti-Candida antibodies. J Immunol Methods. 1980;35(1-2):105–113. doi: 10.1016/0022-1759(80)90155-6. [DOI] [PubMed] [Google Scholar]
  4. Gamble C. N., Reardan J. B. Immunopathogenesis of syphilitic glomerulonephritis. Elution of antitreponemal antibody from glomerular immune-complex deposits. N Engl J Med. 1975 Feb 27;292(9):449–454. doi: 10.1056/NEJM197502272920903. [DOI] [PubMed] [Google Scholar]
  5. Geha R. S. Circulating immune complexes and activation of the complement sequence in acute allergic bronchopulmonary aspergillosis. J Allergy Clin Immunol. 1977 Dec;60(6):357–359. doi: 10.1016/0091-6749(77)90066-5. [DOI] [PubMed] [Google Scholar]
  6. Harkiss G. D., Brown D. L., Evans D. B. Longitudinal study of circulating immune complexes in a patient with Staphylococcus albus-induced shunt nephritis. Clin Exp Immunol. 1979 Aug;37(2):228–238. [PMC free article] [PubMed] [Google Scholar]
  7. Houba V. Immunopathology of nephropathies associated with malaria. Bull World Health Organ. 1975;52(2):199–207. [PMC free article] [PubMed] [Google Scholar]
  8. Inman R. D., Redecha P. B., Knechtle S. J., Schned E. S., van de Rijn I., Christian C. L. Identification of bacterial antigens in circulating immune complexes of infective endocarditis. J Clin Invest. 1982 Aug;70(2):271–280. doi: 10.1172/JCI110614. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaufman D. B., McIntosh R. The pathogenesis of the renal lesion in a patient with streptococcal disease, infected ventriculoatrial shunt, cryogobulinemia and nephritis. Am J Med. 1971 Feb;50(2):262–268. doi: 10.1016/0002-9343(71)90155-0. [DOI] [PubMed] [Google Scholar]
  10. Keslin M. H., Messner R. P., Williams R. C., Jr Glomerulonephritis with subacute bacterial endocarditis. Immunofluorescent studies. Arch Intern Med. 1973 Oct;132(4):578–581. [PubMed] [Google Scholar]
  11. Kilpatrick J. M., Virella G. Development of a single numerical expression for the results of multiple screening tests for immune complexes in diabetic patients: use in statistical comparison of clinically and biochemically defined patient populations. J Clin Lab Immunol. 1982 Apr;7(3):163–166. [PubMed] [Google Scholar]
  12. Levo Y., Gorevic P. D., Kassab H. J., Zucker-Franklin D., Franklin E. C. Association between hepatitis B virus and essential mixed cryoglobulinemia. N Engl J Med. 1977 Jun 30;296(26):1501–1504. doi: 10.1056/NEJM197706302962605. [DOI] [PubMed] [Google Scholar]
  13. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  14. Munoz M., Estes G., Kilpatrick M., Di Salvo A., Virella G. Purification of cytoplasmic antigens from the mycelial phase of Candida albicans: possible advantages of its use in Candida serology. Mycopathologia. 1980 Aug 29;72(1):47–53. doi: 10.1007/BF00443050. [DOI] [PubMed] [Google Scholar]
  15. Ozawa T., Stewart J. A. Immune-complex glomerulonephritis associated with cytomegalovirus infection. Am J Clin Pathol. 1979 Jul;72(1):103–107. doi: 10.1093/ajcp/72.1.103. [DOI] [PubMed] [Google Scholar]
  16. Patrick C. C., Virella G. Isolation of normal human IgG3. Identical molecular weight for normal and monoclonal gamma-3 chains. Immunochemistry. 1978 Feb;15(2):137–139. doi: 10.1016/0161-5890(78)90054-8. [DOI] [PubMed] [Google Scholar]
  17. Patrick C. C., Virella G., Koistinen J., Fudenberg H. H. Differential binding of IgA proteins of different subclasses and allotypes to Staphylococcal protein A. Z Immunitatsforsch Immunobiol. 1977 Dec;153(5):466–469. [PubMed] [Google Scholar]
  18. Perez G. O., Rothfield N., Williams R. C. Immune-complex nephritis in bacterial endocarditis. Arch Intern Med. 1976 Mar;136(3):334–336. [PubMed] [Google Scholar]
  19. Phillips T. M., Draper C. C. Circulating immune complexes in schistosomiasis due to Schistosoma mansoni. Br Med J. 1975 May 31;2(5969):476–477. doi: 10.1136/bmj.2.5969.476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Realdi G., Alberti A., Rigoli A., Tremolada F. Immune-complexes and Australia antigen in cryoglobulinemic sera. Z Immunitatsforsch Exp Klin Immunol. 1974 Jun;147(2):114–126. [PubMed] [Google Scholar]
  21. Reiss E., Stockman L., Kuykendall R. J., Smith S. J. Dissociation of mannan--serum complexes and detection of Candida albicans mannan by enzyme immunoassay variations. Clin Chem. 1982 Feb;28(2):306–310. [PubMed] [Google Scholar]
  22. Segal E., Berg R. A., Pizzo P. A., Bennett J. E. Detection of Candida antigen in sera of patients with candidiasis by an enzyme-linked immunosorbent assay-inhibition technique. J Clin Microbiol. 1979 Jul;10(1):116–118. doi: 10.1128/jcm.10.1.116-118.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Strife C. F., McDonald B. M., Ruley E. J., McAdams A. J., West C. D. Shunt nephritis: the nature of the serum cryoglobulins and their relation to the complement profile. J Pediatr. 1976 Mar;88(3):403–413. doi: 10.1016/s0022-3476(76)80254-5. [DOI] [PubMed] [Google Scholar]
  24. Virella G., Hipp W. A., John J. F., Jr, Kahaleh B., Ford M., Fudenberg H. H. Nephelometric detection of soluble immune complexes: methodology and clinical applications. Int Arch Allergy Appl Immunol. 1979;58(4):402–410. doi: 10.1159/000232219. [DOI] [PubMed] [Google Scholar]
  25. Volanakis J. E., Stroud R. M. Rabbit C1q: purification, functional and structural studies. J Immunol Methods. 1972 Nov;2(1):25–34. doi: 10.1016/0022-1759(72)90014-2. [DOI] [PubMed] [Google Scholar]
  26. Wands J. R., Mann E., Alpert E., Isselbacher K. J. The pathogenesis of arthritis associated with acute hepatitis-B surface antigen-positive hepatitis. Complement activation and characterization of circulating immune complexes. J Clin Invest. 1975 May;55(5):930–936. doi: 10.1172/JCI108022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wands J. R., Perrotto J. L., Isselbacher K. J. Circulating immune complexes and complement sequence activation in infectious mononucleosis. Am J Med. 1976 Feb;60(2):269–272. doi: 10.1016/0002-9343(76)90436-8. [DOI] [PubMed] [Google Scholar]
  28. Weiner M. H., Coats-Stephen M. Immunodiagnosis of systemic candidiasis: mannan antigenemia detected by radioimmunoassay in experimental and human infections. J Infect Dis. 1979 Dec;140(6):989–993. doi: 10.1093/infdis/140.6.989. [DOI] [PubMed] [Google Scholar]
  29. Weiner M. H., Yount W. J. Mannan antigenemia in the diagnosis of invasive Candida infections. J Clin Invest. 1976 Nov;58(5):1045–1053. doi: 10.1172/JCI108555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Yoshinoya S., Cox R. A., Pope R. M. Circulating immune complexes in coccidioidomycosis. Detection and characterization. J Clin Invest. 1980 Oct;66(4):655–663. doi: 10.1172/JCI109901. [DOI] [PMC free article] [PubMed] [Google Scholar]

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