Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1983 Jul;53(1):76–82.

Antibodies to Mi-1 in SLE: relationship to other precipitins and reaction with bovine immunoglobulin.

I N Targoff, G Raghu, M Reichlin
PMCID: PMC1535530  PMID: 6409478

Abstract

Precipitating antibodies to Mi-1, a protein antigen purified from calf thymus nuclear extract, have been reported in a small percentage of patients with dermatomyositis (DM) but not in patients with other connective tissue diseases or controls. Mi-1 shares certain characteristics with immunoglobulin, including the ability to react with rabbit anti-bovine immunoglobulin G (BIgG). A more extensive survey for anti-Mi-1 has been performed, involving 51 normal controls, 67 patients with polymyositis (PM) or DM, 47 patients with rheumatoid arthritis (RA), 41 SLE patients without any other precipitating antibodies to saline extractable tissue antigens on routine testing, and 247 patients, many with SLE, known to have other precipitins. Anti-Mi-1 was found in 13 patients from the latter group (5.25%) and two patients with PM or DM (3.0%), and was not found in other groups. It was found in seven of 95 (7.4%) with anti-nRNP, three of 32 (9.4%) with anti-Sm (with or without anti-nRNP) and three of 120 (2.5%) with anti-Ro (SSA). Anti-Mi-1 cross-reacts directly with BIgG but differs from ordinary rheumatoid factor in its frequent reaction with Fab2 fragments and restricted species specificity. Thus, anti-Mi-1 can be found in patients with diseases other than DM, including SLE, most often in patients with antibodies to nRNP.

Full text

PDF
76

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen C., Elson C. J., Scott D. G., Bacon P. A., Bucknall R. C. IgG antiglobulins in rheumatoid arthritis and other arthritides: relationship with clinical features and other parameters. Ann Rheum Dis. 1981 Apr;40(2):127–131. doi: 10.1136/ard.40.2.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BENNETT G. A., COBB S., JACOX R., JESSAR R. A., ROPES M. W. Proposed diagnostic criteria for rheumatoid arthritis. Bull Rheum Dis. 1956 Dec;7(4):121–124. [PubMed] [Google Scholar]
  3. Bohan A., Peter J. B., Bowman R. L., Pearson C. M. Computer-assisted analysis of 153 patients with polymyositis and dermatomyositis. Medicine (Baltimore) 1977 Jul;56(4):255–286. doi: 10.1097/00005792-197707000-00001. [DOI] [PubMed] [Google Scholar]
  4. Buckley R. H., Dees S. C. Correlation of milk precipitins with IgA deficiency. N Engl J Med. 1969 Aug 28;281(9):465–469. doi: 10.1056/NEJM196908282810903. [DOI] [PubMed] [Google Scholar]
  5. Carr R. I., Wold R. T., Farr R. S. Antibodies to bovine gamma globulin (BGG) and the occurrence of a BGG-like substance in systemic lupus erythematosus sera. J Allergy Clin Immunol. 1972 Jul;50(1):18–30. doi: 10.1016/0091-6749(72)90076-0. [DOI] [PubMed] [Google Scholar]
  6. Clark G., Reichlin M., Tomasi T. B., Jr Characterization of a soluble cytoplasmic antigen reactive with sera from patients with systemic lupus erythmatosus. J Immunol. 1969 Jan;102(1):117–122. [PubMed] [Google Scholar]
  7. Cunningham-Rundles C., Brandeis W. E., Good R. A., Day N. K. Bovine antigens and the formation of circulating immune complexes in selective immunoglobulin A deficiency. J Clin Invest. 1979 Jul;64(1):272–279. doi: 10.1172/JCI109448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cunningham-Rundles C., Brandeis W. E., Safai B., O'Reilly R., Day N. K., Good R. A. Selective IgA deficiency and circulating immune complexes containing bovine proteins in a child with chronic graft versus host disease. Am J Med. 1979 Nov;67(5):883–890. doi: 10.1016/0002-9343(79)90749-6. [DOI] [PubMed] [Google Scholar]
  9. HOLLAND N. H., HONG R., DAVIS N. C., WEST C. D. Significance of precipitating antibodies to milk proteins in the serum of infants and children. J Pediatr. 1962 Aug;61:181–195. doi: 10.1016/s0022-3476(62)80252-2. [DOI] [PubMed] [Google Scholar]
  10. Harrington J. C., Fenton J. W., 2nd, Pert J. H. Polymer-induced precipitation of antigen-antibody complexes: "precipiplex" reactions. Immunochemistry. 1971 May;8(5):413–421. doi: 10.1016/0019-2791(71)90504-0. [DOI] [PubMed] [Google Scholar]
  11. Maddison P. J., Mogavero H., Reichlin M. Patterns of clinical disease associated with antibodies to nuclear ribonucleoprotein. J Rheumatol. 1978 Winter;5(4):407–411. [PubMed] [Google Scholar]
  12. Mattioli M., Reichlin M. Characterization of a soluble nuclear ribonucleoprotein antigen reactive with SLE sera. J Immunol. 1971 Nov;107(5):1281–1290. [PubMed] [Google Scholar]
  13. Milstein C. P., Feinstein A. Comparative studies of two types of bovine immunoglobulin G heavy chains. Biochem J. 1968 Apr;107(4):559–564. doi: 10.1042/bj1070559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
  15. Nishikai M., Reichlin M. Heterogeneity of precipitating antibodies in polymyositis and dermatomyositis. Characterization of the Jo-1 antibody system. Arthritis Rheum. 1980 Aug;23(8):881–888. doi: 10.1002/art.1780230802. [DOI] [PubMed] [Google Scholar]
  16. Nishikai M., Reichlin M. Purification and characterization of a nuclear non-histone basic protein (Mi-1) which reacts with anti-immunoglobulin sera and the sera of patients with dermatomyositis. Mol Immunol. 1980 Sep;17(9):1129–1141. doi: 10.1016/0161-5890(80)90109-1. [DOI] [PubMed] [Google Scholar]
  17. Reichlin M. Current perspectives on serological reactions in SLE patients. Clin Exp Immunol. 1981 Apr;44(1):1–10. [PMC free article] [PubMed] [Google Scholar]
  18. Reichlin M., Mattioli M. Description of a serological reaction characteristic of polymyositis. Clin Immunol Immunopathol. 1976 Jan;5(1):12–20. doi: 10.1016/0090-1229(76)90145-8. [DOI] [PubMed] [Google Scholar]
  19. Venables P. J., Mumford P. A., Maini R. N. Antibodies to nuclear antigens in polymyositis: relationship to autoimmune 'overlap syndromes' and carcinoma. Ann Rheum Dis. 1981 Jun;40(3):217–223. doi: 10.1136/ard.40.3.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wie S. I., Dorrington K. J., Froese A. Characterization of the proteolytic fragments of bovine colostral IgG1. J Immunol. 1978 Jul;121(1):98–104. [PubMed] [Google Scholar]
  21. Wolfe J. F., Adelstein E., Sharp G. C. Antinuclear antibody with distinct specificity for polymyositis. J Clin Invest. 1977 Jan;59(1):176–178. doi: 10.1172/JCI108616. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES