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. 1983 Jul;53(1):192–200.

The detection and specificity of class specific antibodies to whole bacterial cells using a solid phase radioimmunoassay.

C Czerkinsky, A S Rees, L A Bergmeier, S J Challacombe
PMCID: PMC1535533  PMID: 6872327

Abstract

A solid phase radioimmunoassay has been developed which can be used for the detection of isotype specific antibodies to whole bacteria and other particulate antigens, and is applicable to a variety of species. Bacteria are bound to the solid phase by the use either of antibodies, or of methyl glyoxal. Both methods result in a sensitive and reproducible assay, and bacteria do not appear to desorb from the solid phase. The specificity of antibodies to whole bacteria was examined by absorption of antisera with various species of bacteria and retesting, or by determining the binding of antisera to various bacteria bound to the solid phase. Both methods revealed specificity for the bacteria examined. Inhibition studies showed that antibodies to Streptococcus mutans whole cells could be inhibited by purified cell surface antigens glucosyltransferase and antigen I/II, but only minimally by lipoteichoic acid, c polysaccharide or dextran. In murine antisera antibodies of the IgG, IgM, and IgA classes could be detected at amounts of less than 1 ng/ml.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bowden G. H., Hardie J. M., Fillery E. D. Antigens from Actinomyces species and their value in identification. J Dent Res. 1976 Jan;55:A192–A204. doi: 10.1177/002203457605500112011. [DOI] [PubMed] [Google Scholar]
  2. Bratthall D. Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans. Odontol Revy. 1970;21(2):143–152. [PubMed] [Google Scholar]
  3. Brown W. R., Lee E. M. Radioimmunologic measurements of naturally occurring bacterial antibodies. I. Human serum antibodies reactive with Escherichia coli in gastrointestinal and immunologic disorders. J Lab Clin Med. 1973 Jul;82(1):125–136. [PubMed] [Google Scholar]
  4. Carlsson H. E., Hurvell B., Lindberg A. A. Enzyme-linked immunosorbent assay (ELISA) for titration of antibodies against Brucella abortus and Yersinia enterocolitica. Acta Pathol Microbiol Scand C. 1976 Jun;84(3):168–176. doi: 10.1111/j.1699-0463.1976.tb00016.x. [DOI] [PubMed] [Google Scholar]
  5. Catt K., Tregear G. W. Solid-phase radioimmunoassay in antibody-coated tubes. Science. 1967 Dec 22;158(3808):1570–1572. doi: 10.1126/science.158.3808.1570. [DOI] [PubMed] [Google Scholar]
  6. Challacombe S. J., Lehner T. Serum and salivary antibodies to cariogenic bacteria in man. J Dent Res. 1976 Apr;55(Spec No):C139–C148. doi: 10.1177/002203457605500306011. [DOI] [PubMed] [Google Scholar]
  7. Challacombe S. J., Russell M. W., Hawkes J. Passage of intact IgG from plasma to the oral cavity via crevicular fluid. Clin Exp Immunol. 1978 Dec;34(3):417–422. [PMC free article] [PubMed] [Google Scholar]
  8. Challacombe S. J. Serum complement-fixing antibodies in human dental caries. Caries Res. 1974;8(1):84–95. doi: 10.1159/000260095. [DOI] [PubMed] [Google Scholar]
  9. Cohen I. R., Norins L. C. Antibiodies of the IgG, IgM, and IgA classes in newborn and adult sera reactive with gram-negative bacteria. J Clin Invest. 1968 May;47(5):1053–1062. doi: 10.1172/JCI105795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Coykendall A. L. Genetic heterogeneity in Streptococcus mutans. J Bacteriol. 1971 Apr;106(1):192–196. doi: 10.1128/jb.106.1.192-196.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Eggert F. M., Edebo L. B., Gurner B. W., Coombs R. R. A simplified procedure for measuring the class of anti-bacterial antibodies by mixed reverse passive antiglobulin haemagglutination (MRPAH). J Immunol Methods. 1979;26(2):125–140. doi: 10.1016/0022-1759(79)90076-0. [DOI] [PubMed] [Google Scholar]
  12. Gibbons R. J., van Houte J. Dental caries. Annu Rev Med. 1975;26:121–136. doi: 10.1146/annurev.me.26.020175.001005. [DOI] [PubMed] [Google Scholar]
  13. Granfors K., Viljanen M. K., Ahvonen P., Toivanen P. Measurement of IgM and IgG antibodies to Yersinia by solid-phase radioimmunoassay. J Infect Dis. 1978 Aug;138(2):232–236. doi: 10.1093/infdis/138.2.232. [DOI] [PubMed] [Google Scholar]
  14. Kuramitsu H. K. Characterization of cell-associated dextransucrase activity from glucose-grown cells of Streptococcus mutans. Infect Immun. 1974 Jul;10(1):227–235. doi: 10.1128/iai.10.1.227-235.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lehner T., Challacombe S. J., Wilton J. M., Caldwell J. Cellular and humoral immune responses in vaccination against dental caries in monkeys. Nature. 1976 Nov 4;264(5581):69–72. doi: 10.1038/264069a0. [DOI] [PubMed] [Google Scholar]
  16. Linzer R., Gill K., Slade H. D. Chemical composition of Streptococcus mutans type c antigen: comparison to type a, b, and d antigens. J Dent Res. 1976 Jan;55:A109–A115. doi: 10.1177/002203457605500103011. [DOI] [PubMed] [Google Scholar]
  17. MICHAEL J. G., ROSEN F. S. ASSOCIATION OF "NATURAL" ANTIBODIES TO GRAM-NEGATIVE BACTERIA WITH THE GAMMA-1-MACROGLOBULINS. J Exp Med. 1963 Oct 1;118:619–626. doi: 10.1084/jem.118.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McCabe M. M., Smith E. E. Relationship between cell-bound dextransucrase and the agglutination of Streptococcus mutans. Infect Immun. 1975 Sep;12(3):512–520. doi: 10.1128/iai.12.3.512-520.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mestecky J., McGhee J. R., Arnold R. R., Michalek S. M., Prince S. J., Babb J. L. Selective induction of an immune response in human external secretions by ingestion of bacterial antigen. J Clin Invest. 1978 Mar;61(3):731–737. doi: 10.1172/JCI108986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Perch B., Kjems E., Ravn T. Biochemical and serological properties of Streptococcus mutans from various human and animal sources. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):357–370. doi: 10.1111/j.1699-0463.1974.tb02338.x. [DOI] [PubMed] [Google Scholar]
  21. Peters K., Richards F. M. Chemical cross-linking: reagents and problems in studies of membrane structure. Annu Rev Biochem. 1977;46:523–551. doi: 10.1146/annurev.bi.46.070177.002515. [DOI] [PubMed] [Google Scholar]
  22. Rowley M. J. "Natural" antibody in man to flagellar antigens of Salmonella adelaide. Aust J Exp Biol Med Sci. 1970 Apr;48(2):249–252. doi: 10.1038/icb.1970.26. [DOI] [PubMed] [Google Scholar]
  23. Russell M. W., Bergmeier L. A., Zanders E. D., Lehner T. Protein antigens of Streptococcus mutans: purification and properties of a double antigen and its protease-resistant component. Infect Immun. 1980 May;28(2):486–493. doi: 10.1128/iai.28.2.486-493.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Russell M. W., Challacombe S. J., Lehner T. Specificity of antibodies induced by Streptococcus mutans during immunization against dental caries. Immunology. 1980 May;40(1):97–106. [PMC free article] [PubMed] [Google Scholar]
  25. Russell R. R., Beighton D. Specificity of natural antibodies reactive with Streptococcus mutans in monkeys. Infect Immun. 1982 Feb;35(2):741–744. doi: 10.1128/iai.35.2.741-744.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Smith R., Lehner T. A radioimmunoassay for serum and gingival crevicular fluid antibodies to a purified protein of Streptococcus mutans. Clin Exp Immunol. 1981 Feb;43(2):417–424. [PMC free article] [PubMed] [Google Scholar]
  27. Syed S. A., Loesche W. J. Bacteriology of human experimental gingivitis: effect of plaque age. Infect Immun. 1978 Sep;21(3):821–829. doi: 10.1128/iai.21.3.821-829.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Walker J., Colman G., Hughes M. Radioimmunoassay of class-specific antibodies to Streptococcus mutans in monkey serum and saliva. J Immunol Methods. 1979;31(3-4):303–314. doi: 10.1016/0022-1759(79)90143-1. [DOI] [PubMed] [Google Scholar]
  29. Wicken A. J., Gibbens J. W., Knox K. W. Comparative studies on the isolation of membrane lipoteichoic acid from Lactobacillus fermenti. J Bacteriol. 1973 Jan;113(1):365–372. doi: 10.1128/jb.113.1.365-372.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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