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. 1983 Apr;52(1):121–128.

Induction of secondary antibody responses to Plasmodium chabaudi in vitro.

C D Pearson, S A McLean, K Tetley, R S Phillips
PMCID: PMC1535565  PMID: 6861369

Abstract

Spleen cells and peripheral blood mononuclear cells (PBMC) from mice, which had recovered from infection with Plasmodium chabaudi, were induced to produce anti-P. chabaudi antibody by incubating the cells with P. chabaudi parasitized red cells in Marbrook cultures. The anti-malarial antibody was assayed using the indirect fluorescent antibody test. Spleen cells and PBMC from mice infected 2-4 months previously gave higher antibody titres in culture than similar cells from mice infected a year previously. There was a good correlation between the ability of spleen cells or PBMC to be stimulated to produce antibody in vitro and the ability of mice similar to the cell donors to resist a challenge infection. Some immunity can be adoptively transferred with both spleen cells and PBMC.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Callard R. E. Specific in vitro antibody response to influenza virus by human blood lymphocytes. Nature. 1979 Dec 13;282(5740):734–736. doi: 10.1038/282734a0. [DOI] [PubMed] [Google Scholar]
  2. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  3. Dockrell H. M., de Souza J. B., Playfair J. H. The role of the liver in immunity to blood-stage murine malaria. Immunology. 1980 Oct;41(2):421–430. [PMC free article] [PubMed] [Google Scholar]
  4. Gray G. D., Phillips R. S. Use of sorbitol in the cryopreservation of babesia. Res Vet Sci. 1981 May;30(3):388–389. [PubMed] [Google Scholar]
  5. Marbrook J. Primary immune response in cultures of spleen cells. Lancet. 1967 Dec 16;2(7529):1279–1281. doi: 10.1016/s0140-6736(67)90393-5. [DOI] [PubMed] [Google Scholar]
  6. McDonald V., Phillips R. S. Plasmodium chabaudi in mice. Adoptive transfer of immunity with enriched populations of spleen T and B lymphocytes. Immunology. 1978 May;34(5):821–830. [PMC free article] [PubMed] [Google Scholar]
  7. McDonald V., Phillips R. S. Plasmodium chabaudi: adoptive transfer of immunity with different spleen cell populations and development of protective activity in the serum of lethally irradiated recipient mice. Exp Parasitol. 1980 Feb;49(1):26–33. doi: 10.1016/0014-4894(80)90052-1. [DOI] [PubMed] [Google Scholar]
  8. Norby S. W., Alger N. E. Plasmodium berghei: the in vitro immune response. Exp Parasitol. 1981 Feb;51(1):104–115. doi: 10.1016/0014-4894(81)90047-3. [DOI] [PubMed] [Google Scholar]
  9. Playfair J. H., De Souza J. B., Cottrell B. J. Protection of mice against malaria by a killed vaccine: differences in effectiveness against P. yoelii and P. berghei. Immunology. 1977 Oct;33(4):507–515. [PMC free article] [PubMed] [Google Scholar]
  10. Playfair J. H., De Souza J. B., Dockrell H. M., Agomo P. U., Taverne J. Cell-mediated immunity in the liver of mice vaccinated against malaria. Nature. 1979 Dec 13;282(5740):731–734. doi: 10.1038/282731a0. [DOI] [PubMed] [Google Scholar]
  11. Playfair J. H. Immunity to malaria. Br Med Bull. 1982 May;38(2):153–159. doi: 10.1093/oxfordjournals.bmb.a071752. [DOI] [PubMed] [Google Scholar]
  12. Wyler D. J., Brown J. Malaria antigen-specific T-cell responsiveness during infection with Plasmodium falciparum. Clin Exp Immunol. 1977 Sep;29(3):401–407. [PMC free article] [PubMed] [Google Scholar]

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