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. 1991 Sep;85(3):531–535. doi: 10.1111/j.1365-2249.1991.tb05762.x

Corticosteroid can alter antigen expression on alveolar macrophages.

L Marianayagam 1, L W Poulter 1
PMCID: PMC1535593  PMID: 1893636

Abstract

Normal healthy volunteers underwent broncho-alveolar lavage and the cells obtained were cultured for 24 h and 48 h, either alone or in the presence of the corticosteroid, Budesonide. Cell differentials were all normal, the lavages containing greater than 90% alveolar macrophages. Cytospins of these cells were prepared before and after culture. The cytospins were subjected to immunocytochemical analysis using a panel of MoAbs selected to identify subsets of macrophages and functionally relevant surface antigens. In particular, the expression of RFD1 (antigen presenting cell marker) and RFD7 (mature phagocyte marker) were studied. Before culture, BAL macrophages could be divided into two subsets. Of the cells, 39.3% were RFD1+ and 47.2% were RFD7+. Culture with Budesonide was seen to reduce the proportions of RFD1+ cells to 38% while increasing the RFD7+ population to 69% of total. These changes were relatively specific as Budesonide failed to alter the expression of CD68 or Fc(IgG) receptors. Down-regulation of HLA-DR expression was seen, however, after 24 h contact with Budesonide. As these changes could have functional significance, these data support the hypothesis that steroids may have direct effects on the role of alveolar macrophages in immune responses in the lung.

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Selected References

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  1. Ainslie G. M., Poulter L. W., du Bois R. M. Relation between immunocytological features of bronchoalveolar lavage fluid and clinical indices in sarcoidosis. Thorax. 1989 Jun;44(6):501–509. doi: 10.1136/thx.44.6.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Balow J. E., Rosenthal A. S. Glucocorticoid suppression of macrophage migration inhibitory factor. J Exp Med. 1973 Apr 1;137(4):1031–1041. doi: 10.1084/jem.137.4.1031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Campbell D. A., Poulter L. W., Du Bois R. M. Phenotypic analysis of alveolar macrophages in normal subjects and in patients with interstitial lung disease. Thorax. 1986 Jun;41(6):429–434. doi: 10.1136/thx.41.6.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Campbell D. A., Poulter L. W., Janossy G., du Bois R. M. Immunohistological analysis of lung tissue from patients with cryptogenic fibrosing alveolitis suggesting local expression of immune hypersensitivity. Thorax. 1985 Jun;40(6):405–411. doi: 10.1136/thx.40.6.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Erkkilä S., Fröseth B., Hellström P. E., Kaltiokallio K., Taskinen E., Viljanen A., Viljanen B., Selroos O. Inhaled budesonide influences cellular and biochemical abnormalities in pulmonary sarcoidosis. Sarcoidosis. 1988 Sep;5(2):106–110. [PubMed] [Google Scholar]
  6. Gore S. M., Oldham J. A. Randomized trials of high-versus-low-dose steroids in renal transplantation. Does the evidence favor a consensus? Transplantation. 1986 Mar;41(3):319–327. doi: 10.1097/00007890-198603000-00008. [DOI] [PubMed] [Google Scholar]
  7. Guyre P. M., Girard M. T., Morganelli P. M., Manganiello P. D. Glucocorticoid effects on the production and actions of immune cytokines. J Steroid Biochem. 1988;30(1-6):89–93. doi: 10.1016/0022-4731(88)90080-5. [DOI] [PubMed] [Google Scholar]
  8. Hance A. J., Douches S., Winchester R. J., Ferrans V. J., Crystal R. G. Characterization of mononuclear phagocyte subpopulations in the human lung by using monoclonal antibodies: changes in alveolar macrophage phenotype associated with pulmonary sarcoidosis. J Immunol. 1985 Jan;134(1):284–292. [PubMed] [Google Scholar]
  9. Masi A. T. Low dose glucocorticoid therapy in rheumatoid arthritis (RA): transitional or selected add-on therapy? J Rheumatol. 1983 Oct;10(5):675–678. [PubMed] [Google Scholar]
  10. Pauwels R. Mode of action of corticosteroids in asthma and rhinitis. Clin Allergy. 1986 Jul;16(4):281–288. doi: 10.1111/j.1365-2222.1986.tb01959.x. [DOI] [PubMed] [Google Scholar]
  11. Poulter L. W., Campbell D. A., Munro C., Janossy G. Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies. Scand J Immunol. 1986 Sep;24(3):351–357. doi: 10.1111/j.1365-3083.1986.tb02104.x. [DOI] [PubMed] [Google Scholar]
  12. Poulter L. W., Rook G. A., Steele J., Condez A. Influence of 1,25-(OH)2 vitamin D3 and gamma interferon on the phenotype of human peripheral blood monocyte-derived macrophages. Infect Immun. 1987 Sep;55(9):2017–2020. doi: 10.1128/iai.55.9.2017-2020.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rugstad H. E. Antiinflammatory and immunoregulatory effects of glucocorticoids: mode of action. Scand J Rheumatol Suppl. 1988;76:257–264. doi: 10.3109/03009748809102977. [DOI] [PubMed] [Google Scholar]
  14. Salaman J. R. Effects of steroids in combination with other pharmacologic immunosuppressive agents. J Heart Transplant. 1986 Jul-Aug;5(4):304–306. [PubMed] [Google Scholar]
  15. Selroos O. Inhaled corticosteroids and pulmonary sarcoidosis. Sarcoidosis. 1988 Sep;5(2):104–105. [PubMed] [Google Scholar]
  16. Smith K. A. T-cell growth factor. Immunol Rev. 1980;51:337–357. doi: 10.1111/j.1600-065x.1980.tb00327.x. [DOI] [PubMed] [Google Scholar]
  17. Spiteri M. A., Newman S. P., Clarke S. W., Poulter L. W. Inhaled corticosteroids can modulate the immunopathogenesis of pulmonary sarcoidosis. Eur Respir J. 1989 Mar;2(3):218–224. [PubMed] [Google Scholar]
  18. Spiteri M. A., Poulter L. W. Characterization of immune inducer and suppressor macrophages from the normal human lung. Clin Exp Immunol. 1991 Jan;83(1):157–162. doi: 10.1111/j.1365-2249.1991.tb05607.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stosić-Grujicić S., Simić M. M. Modulation of interleukin 1 production by activated macrophages: in vitro action of hydrocortisone, colchicine, and cytochalasin B. Cell Immunol. 1982 May 15;69(2):235–247. doi: 10.1016/0008-8749(82)90070-3. [DOI] [PubMed] [Google Scholar]
  20. du Bois R. M. The alveolar macrophage in sarcoidosis. Sarcoidosis. 1990 Mar;7(1):15–18. [PubMed] [Google Scholar]

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