Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1991 Sep;85(3):469–475. doi: 10.1111/j.1365-2249.1991.tb05751.x

Circulatory antigen of Heymann nephritis. III. Presence of the 70-kD circulatory protein in the immune deposits of Heymann nephritis.

A K Singh 1, M M Schwartz 1
PMCID: PMC1535605  PMID: 1893629

Abstract

An antigen of 70 kD size has been isolated previously from normal rat serum which has immunological cross-reactivity to the Heymann nephritis antigen, F x 1A. Its role in the pathogenesis of Heymann's nephritis was unknown. In this investigation we tested for the presence of 70-kD circulatory antigen in the glomerular immune deposits of Heymann's nephritis. Further, its presence was correlated with severity of disease. It was observed that the presence of the 70-kD antigen strongly correlated with the existence of electron-dense deposits in the lamina rara externa (LRE) of the glomerular capillary wall and with pathologic proteinuria. Temporally, the presence of the 70-kD antigen in the immune deposits was followed by large electron-dense deposits, enhanced complement activity and proteinuria. The data suggest that in the growing immune complex lattice in the LRE, the 70-kD circulatory antigen by virtue of its small size, mobility and antigen cross-reactivity facilitates cross linking and coalescence of immune complexes, resulting in electron-dense immune deposits (EDD) formation which initiates complement activation and consequent proteinuria.

Full text

PDF
469

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abrass C. K., Border W. A., Glassock R. J. Circulating immune complexes in rats with autologous immune complex nephritis. Lab Invest. 1980 Jul;43(1):18–27. [PubMed] [Google Scholar]
  2. Abrass C. K. Evaluation of sequential glomerular eluates from rats with Heymann nephritis. J Immunol. 1986 Jul 15;137(2):530–535. [PubMed] [Google Scholar]
  3. Allegri L., Brianti E., Chatelet F., Manara G. C., Ronco P., Verroust P. Polyvalent antigen-antibody interactions are required for the formation of electron-dense immune deposits in passive Heymann's nephritis. Am J Pathol. 1986 Oct;125(1):1–6. [PMC free article] [PubMed] [Google Scholar]
  4. Alousi M. A., Post R. S., Heymann W. Experimental autoimmune nephrosis in rats. Morphogenesis of the glomerular lesion: immunohistochemical and electron microscopic studies. Am J Pathol. 1969 Jan;54(1):47–71. [PMC free article] [PubMed] [Google Scholar]
  5. Biesecker G., Noble B., Andres G. A., Koffler D. Immunopathogenesis of Heymann's nephritis. Clin Immunol Immunopathol. 1984 Dec;33(3):333–338. doi: 10.1016/0090-1229(84)90304-0. [DOI] [PubMed] [Google Scholar]
  6. Couser W. G., Steinmuller D. R., Stilmant M. M., Salant D. J., Lowenstein L. M. Experimental glomerulonephritis in the isolated perfused rat kidney. J Clin Invest. 1978 Dec;62(6):1275–1287. doi: 10.1172/JCI109248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Edgington T. S., Glassock R. J., Dixon F. J. Autologous immune complex nephritis induced with renal tubular antigen. I. Identification and isolation of the pathogenetic antigen. J Exp Med. 1968 Mar 1;127(3):555–572. doi: 10.1084/jem.127.3.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HEYMANN W., HACKEL D. B., HARWOOD S., WILSON S. G., HUNTER J. L. Production of nephrotic syndrome in rats by Freund's adjuvants and rat kidney suspensions. Proc Soc Exp Biol Med. 1959 Apr;100(4):660–664. doi: 10.3181/00379727-100-24736. [DOI] [PubMed] [Google Scholar]
  9. Hogendoorn P. C., Bruijn J. A., vd Broek L. J., De Heer E., Foidart J. M., Hoedemaeker P. J., Fleuren G. J. Antibodies to purified renal tubular epithelial antigens contain activity against laminin, fibronectin, and type IV collagen. Lab Invest. 1988 Mar;58(3):278–286. [PubMed] [Google Scholar]
  10. Jeraj K., Vernier R. L., Sisson S. P., Michael A. F. A new glomerular antigen in passive Heymann's nephritis. Br J Exp Pathol. 1984 Aug;65(4):485–498. [PMC free article] [PubMed] [Google Scholar]
  11. Kagami S., Kawakami K., Okada K., Kuroda Y., Morioka T., Shimizu F., Oite T. Mechanism of formation of subepithelial electron-dense deposits in active in situ immune complex glomerulonephritis. Am J Pathol. 1990 Mar;136(3):631–639. [PMC free article] [PubMed] [Google Scholar]
  12. Kanalas J. J., Makker S. P. A possible ligand of serum origin for the kidney autoantigen of Heymann nephritis. J Immunol. 1988 Dec 15;141(12):4152–4157. [PubMed] [Google Scholar]
  13. Kerjaschki D., Farquhar M. G. The pathogenic antigen of Heymann nephritis is a membrane glycoprotein of the renal proximal tubule brush border. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5557–5561. doi: 10.1073/pnas.79.18.5557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Makker S. P., Singh A. K. Characterization of the antigen (gp600) of Heymann nephritis. Lab Invest. 1984 Mar;50(3):287–293. [PubMed] [Google Scholar]
  15. Ronco P., Allegri L., Melcion C., Pirotsky E., Appay M. D., Bariety J., Pontillon F., Verroust P. A monoclonal antibody to brush border and passive Heymann nephritis. Clin Exp Immunol. 1984 Feb;55(2):319–332. [PMC free article] [PubMed] [Google Scholar]
  16. Ronco P., Neale T. J., Wilson C. B., Galceran M., Verroust P. An immunopathologic study of a 330-kD protein defined by monoclonal antibodies and reactive with anti-RTE alpha 5 antibodies and kidney eluates from active Heymann nephritis. J Immunol. 1986 Jan;136(1):125–130. [PubMed] [Google Scholar]
  17. Rydel J. J., Schwartz M. M., Singh A. K. Sequential localization of antibody to multiple regions of the glomerular capillary wall in passive Heymann nephritis. Lab Invest. 1989 Apr;60(4):492–498. [PubMed] [Google Scholar]
  18. Salant D. J., Belok S., Madaio M. P., Couser W. G. A new role for complement in experimental membranous nephropathy in rats. J Clin Invest. 1980 Dec;66(6):1339–1350. doi: 10.1172/JCI109987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schumaker V. N., Zavodszky P., Poon P. H. Activation of the first component of complement. Annu Rev Immunol. 1987;5:21–42. doi: 10.1146/annurev.iy.05.040187.000321. [DOI] [PubMed] [Google Scholar]
  20. Singh A. K., Makker S. P. Circulatory antigens of Heymann nephritis. I. Identification and partial characterization. Immunology. 1986 Mar;57(3):467–472. [PMC free article] [PubMed] [Google Scholar]
  21. Singh A. K., Schwartz M. M. Circulatory antigen of Heymann nephritis. II. Isolation of a 70,000 MW antigen from normal rat serum which cross-reacts with Heymann nephritis antigen. Immunology. 1986 Nov;59(3):451–458. [PMC free article] [PubMed] [Google Scholar]
  22. Singh A. K., Schwartz M. M. Nephritogenicity and immunocytochemical localization of the 70-kilodalton glycoprotein subunit (gp70) of Heymann antigen. Clin Immunol Immunopathol. 1988 Jul;48(1):61–77. doi: 10.1016/0090-1229(88)90157-2. [DOI] [PubMed] [Google Scholar]
  23. Van Damme B. J., Fleuren G. J., Bakker W. W., Vernier R. L., Hoedemaeker P. J. Experimental glomerulonephritis in the rat induced by antibodies directed against tubular antigens. V. Fixed glomerular antigens in the pathogenesis of heterologous immune complex glomerulonephritis. Lab Invest. 1978 Apr;38(4):502–510. [PubMed] [Google Scholar]
  24. de Heer E., Daha M. R., Bhakdi S., Bazin H., van Es L. A. Possible involvement of terminal complement complex in active Heymann nephritis. Kidney Int. 1985 Feb;27(2):388–393. doi: 10.1038/ki.1985.21. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES