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. 1991 Sep;85(3):453–458. doi: 10.1111/j.1365-2249.1991.tb05748.x

Pigeon breeders' lung: pigeon intestinal mucin, an antigen distinct from pigeon IgA.

A Todd 1, R M Coan 1, A Allen 1
PMCID: PMC1535616  PMID: 1893627

Abstract

Antigens identified by indirect immunofluorescence staining and specific for sera from patients with pigeon breeders' lung or healthy pigeon breeders, have been isolated from pigeon intestinal mucus. Two antigenic peaks, one pigeon intestinal mucin and the other IgA, were separated by equilibrium centrifugation of water-soluble mucus in a caesium chloride density gradient. Antigenic positive material was identified by a modified double-sandwich ELISA, by inhibition of haemagglutination of turkey erythrocytes and by gel diffusion. Antigenic-positive material co-fractionated on gel-filtration with purified intact and papain digested pigeon mucin, both free of IgA. These studies demonstrate antibodies to two quite different antigens are associated specifically with sera from pigeon breeders, a novel antigen pigeon intestinal mucin and the previously documented pigeon IgA.

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Selected References

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  1. BARBORIAK J. J., SOSMAN A. J., REED C. E. SEROLOGICAL STUDIES IN PIGEON BREEDER'S DISEASE. J Lab Clin Med. 1965 Apr;65:600–604. [PubMed] [Google Scholar]
  2. Banham S. W., McKenzie H., McSharry C., Lynch P. P., Boyd G. Antibody against a pigeon bloom-extract: a further antigen in pigeon fanciers' lung. Clin Allergy. 1982 Mar;12(2):173–178. doi: 10.1111/j.1365-2222.1982.tb01636.x. [DOI] [PubMed] [Google Scholar]
  3. Diment J. A., Pepys J. Avian erythrocyte agglutination tests with the sera of bird fanciers. J Clin Pathol. 1977 Jan;30(1):29–34. doi: 10.1136/jcp.30.1.29. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Faux J. A., Holford-Strevens V., Wells I. D., Pepys J. 'False positive' precipitation reactions to extracts of organic dusts due to a teichoic acid from S. aureus. Techniques. Clin Exp Immunol. 1970 Dec;7(6):897–902. [PMC free article] [PubMed] [Google Scholar]
  5. Faux J. A., Wells I. D., Pepys J. Specificity of avian serum proteins in tests against the sera of bird fanciers. Clin Allergy. 1971 Jun;1(2):159–170. doi: 10.1111/j.1365-2222.1971.tb03015.x. [DOI] [PubMed] [Google Scholar]
  6. Fink J. N., Sosman A. J., Barboriak J. J., Schlueter D. P., Holmes R. A. Pigeon breeders' disease. A clinical study of a hypersensitivity pneumonitis. Ann Intern Med. 1968 Jun;68(6):1205–1219. doi: 10.7326/0003-4819-68-6-1205. [DOI] [PubMed] [Google Scholar]
  7. Fink J. N., Tebo T., Barboriak J. J. Differences in the immune responses of pigeon breeders to pigeon serum proteins. J Lab Clin Med. 1969 Aug;74(2):325–330. [PubMed] [Google Scholar]
  8. Fredricks W. W., Tebo T. H. The antigens of pigeon breeder's disease. I. Studies on unfractionated pigeon dropping extracts. Int Arch Allergy Appl Immunol. 1977;53(3):214–221. doi: 10.1159/000231755. [DOI] [PubMed] [Google Scholar]
  9. Fredricks W. W., Tebo T. H. The antigens of pigeon breeder's disease. III. Immunologically related antigens of pigeon dropping extracts: PDE1,2,A and B. Int Arch Allergy Appl Immunol. 1980;61(1):65–74. [PubMed] [Google Scholar]
  10. Hargreave F. E., Pepys J. Allergic respiratory reactions in bird fanciers provoked by allergen inhalation provocation tests. Relation to clinical features and allergic mechanisms. J Allergy Clin Immunol. 1972 Sep;50(3):157–173. doi: 10.1016/0091-6749(72)90047-4. [DOI] [PubMed] [Google Scholar]
  11. Hensley G. T., Garancis J. C., Cherayil G. D., Fink J. N. Lung biopsies of pigeon breeders' disease. Arch Pathol. 1969 Jun;87(6):572–579. [PubMed] [Google Scholar]
  12. Hutton D. A., Pearson J. P., Allen A., Foster S. N. Mucolysis of the colonic mucus barrier by faecal proteinases: inhibition by interacting polyacrylate. Clin Sci (Lond) 1990 Mar;78(3):265–271. doi: 10.1042/cs0780265. [DOI] [PubMed] [Google Scholar]
  13. Longbottom J. L. Pigeon breeders' disease: quantitative immunoelectrophoretic studies of pigeon bloom antigen. Clin Exp Allergy. 1989 Nov;19(6):619–624. doi: 10.1111/j.1365-2222.1989.tb02756.x. [DOI] [PubMed] [Google Scholar]
  14. Mantle M., Allen A. A colorimetric assay for glycoproteins based on the periodic acid/Schiff stain [proceedings]. Biochem Soc Trans. 1978;6(3):607–609. doi: 10.1042/bst0060607. [DOI] [PubMed] [Google Scholar]
  15. Mantle M., Allen A. Isolation and characterization of the native glycoprotein from pig small-intestinal mucus. Biochem J. 1981 Apr 1;195(1):267–275. doi: 10.1042/bj1950267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mantle M., Mantle D., Allen A. Polymeric structure of pig small-intestinal mucus glycoprotein. Dissociation by proteolysis or by reduction of disulphide bridges. Biochem J. 1981 Apr 1;195(1):277–285. doi: 10.1042/bj1950277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mestecky J., McGhee J. R. Immunoglobulin A (IgA): molecular and cellular interactions involved in IgA biosynthesis and immune response. Adv Immunol. 1987;40:153–245. doi: 10.1016/s0065-2776(08)60240-0. [DOI] [PubMed] [Google Scholar]
  18. Munro A. C., Inglis G., Lynch P. P., Boyd G. A survey of P1-antibodies in Scottish pigeon fanciers. Clin Allergy. 1980 Nov;10(6):643–650. doi: 10.1111/j.1365-2222.1980.tb02147.x. [DOI] [PubMed] [Google Scholar]
  19. Sennekamp J., Vogel F., Stiens R. Detection of IgG antibodies against pigeon intestinal mucosa antigens in pigeon breeders' sera using the immunofluorescent technique. Int Arch Allergy Appl Immunol. 1976;50(6):674–683. doi: 10.1159/000231546. [DOI] [PubMed] [Google Scholar]
  20. Tebo T. H., Fredricks W. W., Roberts R. C. The antigens of pigeon breeder's disease. II. Isolation and characterization of antigen PDE1. Int Arch Allergy Appl Immunol. 1977;54(6):553–559. doi: 10.1159/000231876. [DOI] [PubMed] [Google Scholar]
  21. Tebo T. H., Moore V. L., Fink J. N. Antigens in pigeon breeder's disease: the use of pigeon dropping antigens in detecting antibody activity. Clin Allergy. 1977 Mar;7(2):103–108. doi: 10.1111/j.1365-2222.1977.tb01430.x. [DOI] [PubMed] [Google Scholar]
  22. Yang J. P., Purtilo D. T. Pigeon breeders' disease: immunofluorescent localization of sensitizing antigens in pigeon. Clin Immunol Immunopathol. 1975 May;4(1):46–51. doi: 10.1016/0090-1229(75)90038-0. [DOI] [PubMed] [Google Scholar]

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